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Leucas aspera Nanomedicine Shows Superior Toxicity and Cell Migration Retarded in Prostate Cancer Cells

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Abstract

Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in PC3 prostate cancer cells and normal cells. L. aspera is a plant that is used in ayurveda due to the antirheumatic, antipyretic, anti-inflammatory, antibacterial, anticancer, and cytotoxic activities. Nanoparticles of L. aspera were prepared from plant methanolic extracts. Cytotoxic effect was studied in the normal and prostate cancer cells. Size and morphology of the formulated nanoparticles was assessed using dynamic light scattering and scanning electron microscopy. In vitro cytotoxicity of L. aspera nanoparticles for PC3 cells was concentration- and time-dependent. In vitro hemolysis assay, cellular uptake studies, cell aggregation studies, and cell migration assay established the anticancerous activity of L. aspera in prostate cancer.

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Acknowledgments

Vinoth-Kumar Lakshmanan is grateful to Department of Science and Technology, India, for providing the fund and guidelines to this work. Authors also acknowledge the financial support in the form of a M-Tech grant from Department of Science and Technology. We thank lab members K.S. Snima, Pratheeksha Pillai, Sajin Ravi and Cochin University of Science and Technology for help in guidelines, and the SEM, DLS, and TGA procedures.

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Authors and Affiliations

  1. Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi Campus, Kochi, Kerala, 682041, India

    Anjusha Mohan, Shantikumar V. Nair & Vinoth-Kumar Lakshmanan

  2. Department of Biomedical Sciences, Chonnam National University Medical School, Chonnam National University, 160 Baeksuh-Roh, Dong-Gu, Gwangju, 61469, Republic of Korea

    Vinoth-Kumar Lakshmanan

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  1. Anjusha Mohan
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Correspondence to Vinoth-Kumar Lakshmanan.

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Mohan, A., Nair, S.V. & Lakshmanan, VK. Leucas aspera Nanomedicine Shows Superior Toxicity and Cell Migration Retarded in Prostate Cancer Cells. Appl Biochem Biotechnol 181, 1388–1400 (2017). https://doi.org/10.1007/s12010-016-2291-5

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