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Microaerophilic Symmetric Reductive Cleavage of Reactive Azo Dye—Remazole Brilliant Violet 5R by Consortium VIE6: Community Synergism

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Abstract

The textile-dyeing industry is rated as one of the foremost industrial sectors that explodes large amount of pollutants to the environment. Reactive azo dye degradation, being a major constituent of these pollutants and perilous material, has been constantly receiving scientific attention. In textile industry, use of Remazole Brilliant Violet 5R (RBV5R) as reactive azo dyes is more frequent. Highly competent, RBV5R-degrading bacterial consortium VIE6 was developed from the soil of the Vatva Industrial Estate, Gujarat, India. Consortium VIE6 comprised of five bacterial strains Bacillus sp. DMB1, Staphylococcus sp. DMB2, Escherichia sp. DMB3, Enterococcus sp. DMB4, and Pseudomonas sp. DMB5. These strains convened a better decolorization efficiency between 200 and 1000 mg/L of dye concentration and were much stable at pH 6.5, 37 °C. Azoreductase, laccase, and lignin peroxidase activities of consortium showed significant variation throughout the degradation process indicating the different metabolic capabilities of the existing microflora. The community interactions and synergism were shown to facilitate the biotransformation of RBV5R by combination of various electron donors. Voltammograms revealed the variations in electron discharge properties which coincide with the dynamics of community derived using qPCR assays. The variation in catabolic capabilities of the individual strains was observed during active metabolism of RBV5R degradation pertaining to the aerobic and facultative functions.

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Acknowledgments

This work was supported by the Department of Biotechnology (DBT) grant from Ministry of Science and Technology, New Delhi, India. Authors gratefully acknowledge Sophisticated Instrumentation Centre for Applied Research and Training (SICART), Vallabh Vidyanagar, Gujarat, India, for providing FTIR facility. Department of Chemistry, Sardar Patel University, is also duly acknowledged for extending their NMR facility.

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Authors and Affiliations

  1. Environmental Genomics and Proteomics Lab, UGC-Centre of Advanced Study, Post Graduate Department of Biosciences, Sardar Patel University, Satellite Campus, Vadtal Road, Bakrol, Anand, 388 315, Gujarat, India

    Binal Shah, Kunal Jain, Hardik Jiyani & Datta Madamwar

  2. Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad, 500 007, India

    Venkata Mohan

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  1. Binal Shah
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  2. Kunal Jain
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  3. Hardik Jiyani
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  4. Venkata Mohan
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  5. Datta Madamwar
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Correspondence to Datta Madamwar.

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The authors declare that they have no conflict of interest.

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Shah, B., Jain, K., Jiyani, H. et al. Microaerophilic Symmetric Reductive Cleavage of Reactive Azo Dye—Remazole Brilliant Violet 5R by Consortium VIE6: Community Synergism. Appl Biochem Biotechnol 180, 1029–1042 (2016). https://doi.org/10.1007/s12010-016-2150-4

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