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Lipase from Solvent-Tolerant Pseudomonas sp. DMVR46 Strain Adsorb on Multiwalled Carbon Nanotubes: Application for Enzymatic Biotransformation in Organic Solvents

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Abstract

Immobilization of biocatalysts onto particulate carriers has been widely explored for recycling of biocatalyst. However, surface properties often affect the amount of biocatalysts immobilized, their bioactivity and stability, hampering their wide applications. The aim of this work was to elucidate the importance of nanoimmobilization system in organic synthesis. The surface of multiwalled carbon nanotubes (MWCNTs) was functionalized with a mixture of concentrated acids to create an interface for enzyme immobilization. Successful functionalization and enzyme immobilization was structurally evidenced by transmision electron microscopy analysis and Fourier-transform infrared spectroscopy analysis. Furthermore, immobilized enzyme was exploited for the synthesis of flavoured ester ethyl butyrate in the presence of n-heptane. Optimized conditions for enhanced ester synthesis was found to be 8.5 pH, 40 °C, 150 rpm, 0.15:0.2 M substrate molar ratio (ethanol/butyric acid) and n-heptane as reaction medium. Utmost 81 % of ester synthesis was obtained using immobilized lipase quite higher in comparison to that of free lipase. The activation energy indicated a lower energy requirement for immobilization of lipase on the surface of functionalized MWCNTs. In summary, immobilization of lipase on functionalized MWCNTs by simple adsorption method displayed excellent properties for enzyme stability and reusability, indicating its potential for application in organic synthesis.

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Acknowledgments

The authors would like to acknowledge University Grants Commission (UGC) grant no. F. 42-167/2013 (SR), New Delhi, for financial support. The authors would also like to acknowledge SICART, Vallabh Vidhyanagar, for FTIR and TEM facility and Department of Material Sciences, Vallabh Vidhyanagar, for providing MWCNTs.

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  1. Environmental Genomics and Proteomics Laboratory, BRD School of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box # 39, Vallabh Vidhyanagar, 388 120, Gujarat, India

    Patel Vrutika & Madamwar Datta

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Correspondence to Madamwar Datta.

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Vrutika, P., Datta, M. Lipase from Solvent-Tolerant Pseudomonas sp. DMVR46 Strain Adsorb on Multiwalled Carbon Nanotubes: Application for Enzymatic Biotransformation in Organic Solvents. Appl Biochem Biotechnol 177, 1313–1326 (2015). https://doi.org/10.1007/s12010-015-1816-7

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  • DOI: https://doi.org/10.1007/s12010-015-1816-7

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