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Vertical Profiles of Community Abundance and Diversity of Anaerobic Methanotrophic Archaea (ANME) and Bacteria in a Simple Waste Landfill in North China

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An Erratum to this article was published on 08 October 2015

Abstract

Anaerobic methane oxidation (AMO) is considered to be an important sink of CH4 in habitats as marine sediments. But, few studies focused on AMO in landfills which may be an important sink of CH4 derived from waste fermentation. To show evidence of AMO and to uncover function anaerobic methanotroph (ANME) community in landfill, different age waste samples were collected in Jinqianpu landfill located in north China. Through high-throughput sequencing, Methanomicrobiales and Methanosarcinales archaea associated with ANME and reverse methanogenic archaea of Methanosarcina and Methanobacterium were detected. Sulfate-reducing bacteria (SRB) (Desulfobulbus and Desulfococcus) which could couple with ANME-conducting AMO were also found. But, the community structure of ANME had no significant difference with depths. From the results of investigation, we can come to a conclusion that sulfate-dependent anaerobic methane oxidation (SR-DAMO) would be the dominant AMO process in the landfill, while iron-dependent anaerobic methane oxidation (M/IR-DAMO) process was weak though concentration of ferric iron was large in the landfill. Denitrification-dependent anaerobic methane oxidation (NR-DAMO) was negative because of lack of nitrate and relevant function microorganisms in the landfill. Results also indicate that CH4 mitigation would have higher potential by increasing electron acceptor contents and promoting the growth of relevant function microorganisms.

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Acknowledgement

This research was funded by National Natural Science Foundation of China (Grant Numbers 41402206 and 41272253); Natural Science Foundation of Jilin Province (No.20130101027JC); Youth Science Foundation of Jilin Province (No. 20140520152JH). The authors are grateful to the support of China postdoctoral Science Foundation (No. 2013M541311); and Key Laboratory for Solid Waste Management and Environment Safety Open Fund (Grant Number SWMES 2013-07).

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Authors and Affiliations

  1. Key Lab of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun, 130021, People’s Republic of China

    Jun Dong, Linjie Ding, Xu Wang, Zifang Chi & Jiansen Lei

  2. Key Laboratory for Solid Waste Management and Environment Safety, Ministry of Education, Tsinghua University, Beijing, 100084, People’s Republic of China

    Zifang Chi

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  1. Jun Dong
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  2. Linjie Ding
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Correspondence to Zifang Chi.

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Dong, J., Ding, L., Wang, X. et al. Vertical Profiles of Community Abundance and Diversity of Anaerobic Methanotrophic Archaea (ANME) and Bacteria in a Simple Waste Landfill in North China. Appl Biochem Biotechnol 175, 2729–2740 (2015). https://doi.org/10.1007/s12010-014-1456-3

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