Skip to main content
SpringerLink
Log in

Greater Taxol Yield of Fungus Pestalotiopsis hainanensis from Dermatitic Scurf of the Giant Panda (Ailuropoda melanoleuca)

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

While taxol yields of fungi from non-animal sources are still low, whether Pestalotiopsis hainanensis isolated from the scurf of a dermatitic giant panda, Ailuropoda melanoleuca, provides a greater taxol yield remains unknown. The objective of the study was to determine the corresponding taxol yield. The structure of the taxol produced by the fungus was evaluated by thin layer chromatography (TLC), ultraviolet (UV) spectroscopy, high-performance liquid chromatography (HPLC), 1H and 13C nuclear magnetic resonance spectroscopy (1H-NMR and 13C-NMR), and time-of-flight mass spectrometry (TOF-MS), with standard taxol as a control. The results demonstrated that the P. hainanensis fungus produced taxol, which had the same structure as the standard taxol and yield of 1,466.87 μg/L. This fungal taxol yield from the dermatitic giant panda was significantly greater than those of fungus from non-animal sources. The taxol-producing fungus may be a potential candidate for the production of taxol on an industrial scale.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Bi, J., Ji, Y., Pan, J., Yu, Y., Chen, H., & Zhu, X. (2013). A new taxol-producing fungus (Pestalotiopsis malicola) and evidence for taxol as a transient product in the culture. African Journal of Biotechnology, 10(34), 6647–6654.

    Google Scholar 

  2. Chmurny, G. N., Hilton, B. D., Brobst, S., Look, S. A., Witherup, K. M., & Beutler, J. A. (1992). 1H- and 13C-NMR assignments for taxol, 7-epi-taxol, and cephalomannine. Journal of Natural Products, 55(4), 414–423.

    Article  CAS  Google Scholar 

  3. Fuse, S., Miyamoto, S., Nakai, K., Sasuga, D., & Takahashi, T. (2006). A formal total synthesis of taxol aided by an automated synthesizer. Chemistry--An Asian Journal, 1(3), 370–383.

    Article  Google Scholar 

  4. Gangadevi, V., & Muthumary, J. (2009). Taxol production by Pestalotiopsis terminaliae, an endophytic fungus of Terminalia arjuna (arjun tree). Biotechnology and Applied Biochemistry, 52(1), 9–15.

    Article  CAS  Google Scholar 

  5. Gu, Y., Ruan, J. X., Ma, X. P., Li, D. S., Liu, X. M., Ling, S. S., Li, C. C., Tong, Y. F., & Wang, C. D. (2014). A Pestalotiopsis hainanensis strain from the body surface of Ailuropoda melanoleuca. Mycosystema, 33(3), 731–737.

    CAS  Google Scholar 

  6. Huang, F., Han, T., & Lp, Q. (2011). Isolation and identification of endophytic fungi from Vitex negundo var. cannabifolia. China Journal of Chinese Material Medical, 36(14), 1945–1950.

    Google Scholar 

  7. Ishihara, Y., & Baran, P. S. (2010). Two-phase terpene total synthesis: historical perspective and application to the taxol problem. Synlett, 2010(12), 1733–1745.

    Article  Google Scholar 

  8. Kathiravan, G., & Sri Raman, V. (2010). In vitro TAXOL production, by Pestalotiopsis breviseta—a first report. Fitoterapia, 81(6), 557–564.

    Article  CAS  Google Scholar 

  9. Kumaran, R. S., Kim, H. J., & Hur, B.-K. (2010). Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata. Journal of Bioscience and Bioengineering, 110(5), 541–546.

    Article  CAS  Google Scholar 

  10. Li, J.-Y., Sidhu, R., Bollon, A., & Strobel, G. (1998). Stimulation of taxol production in liquid cultures of Pestalotiopsis microspora. Mycological Research, 102, 461–464.

    Article  CAS  Google Scholar 

  11. J-y, L., Strobel, G., Sidhu, R., Hess, W. M., & Ford, E. J. (1996). Endophytic taxol-producing fungi from bald cypress, Taxodium distichum. Microbiology, 142(8), 2223–2226.

    Article  Google Scholar 

  12. Li, J. Y., Sidhu, R. S., Ford, E. J., Long, D. M., Hess, W. M., & Strobel, G. A. (1998). The induction of taxol production in the endophytic fungus—Periconia sp from Torreya grandifolia. Journal of Industrial Microbiology and Biotechnology, 20(5), 259–264.

    Article  CAS  Google Scholar 

  13. Liu, A. R., Xu, T., & Guo, L. D. (2007). Molecular and morphological description of Pestalotiopsis hainanensis sp nov., a new endophyte from a tropical region of China. Fungal Diversity, 24, 23–36.

    CAS  Google Scholar 

  14. Liu, K., Ding, X., Deng, B., & Chen, W. (2009). Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis. Journal of Industrial Microbiology and Biotechnology, 36(9), 1171–1177.

    Article  CAS  Google Scholar 

  15. Lou, J., Niu, X.-L., Yan, F., Pan, J., & Zhu, X.-D. (2011). Recent progresses in the studies of taxol and taxane-producing fungi. Mycosystema, 30, 158–167.

    CAS  Google Scholar 

  16. Noh, M. J., Yang, J. G., Kim, K. S., Yoon, Y. M., Kang, K., Han, H. Y., Shim, S. B., & Park, H. J. (1999). Isolation of a novel microorganism, Pestalotia heterocornis, producing paclitaxel. Biotechnology and Bioengineering, 64(5), 620–623.

    Article  CAS  Google Scholar 

  17. Shrestha, K., Strobel, G. A., Shrivastava, S. P., & Gewali, M. B. (2001). Evidence for paclitaxel from three new endophytic fungi of Himalayan yew of Nepal. Planta Medica, 67(04), 374–376.

    Article  CAS  Google Scholar 

  18. Sonaimuthu, V., Krishnamoorthy, S., & Johnpaul, M. (2010). Optimization of process parameters for improved production of Taxol by a novel endophytic fungus Pestalotiopsis oxyanthi SVJM060 isolated from Taxus baccta. Journal of Biotechnology, 150, S471–S471.

    Article  Google Scholar 

  19. Stierle, A., Strobel, G., & Stierle, D. (1993). Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science, 260(5105), 214–216.

    Article  CAS  Google Scholar 

  20. Strobel, G., Yang, X., Sears, J., Kramer, R., Sidhu, R. S., & Hess, W. M. (1996). Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiology, 142(Pt 2), 435–440.

    Article  CAS  Google Scholar 

  21. Strobel, G. A., Hess, W., Li, J.-Y., Ford, E., Sears, J., Sidhu, R. S., & Summerell, B. (1997). Pestalotiopsis guepinii, a taxol-producing endophyte of the wollemi pine, Wollemia nobilis. Australian Journal of Botany, 45(6), 1073–1082.

    Article  CAS  Google Scholar 

  22. Sun, D., Ran, X., & Wang, J. (2008). Isolation and identification of a taxol-producing endophytic fungus from Podocarpus. Acta Microbiologica Sinica, 48(5), 589–595.

    CAS  Google Scholar 

  23. Vennila, R., & Muthumary, J. (2011). Taxol from Pestalotiopsis pauciseta VM1, an endophytic fungus of Tabebuia pentaphylla. Biomedicine & Preventive Nutrition, 1(2), 103–108.

    Article  Google Scholar 

  24. Wang, J., Li, G., Lu, H., Zheng, Z., Huang, Y., & Su, W. (2000). Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei. FEMS Microbiology Letters, 193(2), 249–253.

    Article  CAS  Google Scholar 

  25. Wani, M. C., Taylor, H. L., Wall, M. E., Coggon, P., & McPhail, A. T. (1971). Plant antitumor agents. VI. Isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. Journal of the American Chemical Society, 93(9), 2325–2327.

    Article  CAS  Google Scholar 

  26. Yang, F. (2010). Animal nutrition (the second edition). Beijing: China Agriculture Press.

    Google Scholar 

Download references

Acknowledgments

This study was supported by the Program for Youth Fund of Sichuan Provincial Education Department (12ZB093); National Key Technology R&D Program of China (2012BAC01B06); The giant panda international cooperation project funds (AD1415); and Applied Basic Research Project in Sichuan Province (2013TY0175). This study is a part of undergraduate graduation thesis breeding project of Sichuan Agricultural University. Examinations of taxol and samples of HPLC, 1H and 13C-NMR, and TOF-MS tests are performed in the Chengdu Institute of Biology, Chinese Academy of Sciences. Thanks for them. All authors have agreed to submit this manuscript to the Applied Biochemistry and Biotechnology.

Author information

Authors and Affiliations

  1. College of Life Sciences, Sichuan Agricultural University, Ya’an, 625014, China

    Yu Gu

  2. College of Sciences, Sichuan Agricultural University, Ya’an, 625014, China

    Yanlin Wang & Guizhou Yue

  3. College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, 625014, China

    Xiaoping Ma, Yuetian Zhang, Yunyan Zhang, Shanshan Li, Xintian Wen, Sanjie Cao, Xiaobo Huang, Junliang Deng, Zhicai Zuo, Shumin Yu, Liuhong Shen & Rui Wu

  4. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China

    Xiaoping Ma

  5. China Conservation and Research Center for Giant Panda, Wolong, 623006, China

    Chengdong Wang, Shanshan Ling & Xiaomin Liu

Authors
  1. Yu Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yanlin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoping Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chengdong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guizhou Yue
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuetian Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yunyan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Shanshan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shanshan Ling
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Xiaomin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Xintian Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Sanjie Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Xiaobo Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Junliang Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Zhicai Zuo
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Shumin Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  17. Liuhong Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  18. Rui Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xiaoping Ma or Chengdong Wang.

Additional information

Xiaoping Ma and Chengdong Wang contributed equally to this work, tied for Xiaoping Ma.

Yu Gu and Yanlin Wang contributed equally to this work and should be considered as joint first authors, tied for Yu Gu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gu, Y., Wang, Y., Ma, X. et al. Greater Taxol Yield of Fungus Pestalotiopsis hainanensis from Dermatitic Scurf of the Giant Panda (Ailuropoda melanoleuca). Appl Biochem Biotechnol 175, 155–165 (2015). https://doi.org/10.1007/s12010-014-1254-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-014-1254-y

Keywords

Search

Navigation