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The Prognostic Value of Histidine-Rich Glycoprotein RNA in Breast Tissue Using Unmodified Gold Nanoparticles Assay

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Abstract

The aim of is this study is to explore the role of tissue histidine-rich glycoprotein (HRG) RNA as a promising clinically useful biomarker for breast cancer patients prognosis using nanogold assay. Expression of the HRG RNA was assessed by gold nanoparticles and conventional RT-PCR after purification by magnetic nanoparticles in breast tissue samples. The study included 120 patients, 60 of which were histologically proven breast carcinoma cases, 30 had benign breast lesions and 30 were healthy individuals who had undergone reductive plastic surgery. ER, PR and HER2 status were also investigated. The prognostic significance of tissue HRG RNA expression in breast cancer was explored. The magnetic nanoparticles coated with specific thiol modified oligonucleotide probe were used successfully in purification of HRG RNA from breast tissue total RNAs with satisfactory yield. The developed HRG AuNPs assay had a sensitivity and a specificity of 90 %, and a detection limit of 1.5 nmol/l. The concordance rate between the HRG AuNPs assay with RT-PCR after RNA purification using magnetic nanoparticles was 93.3 %. The median follow-up period was 60 months. Among traditional prognostic biomarkers, HRG was a significant independent prognostic marker in relapse-free survival (RFS). HRG RNA is an independent prognostic marker for breast cancer and can be detected using gold NPs assay, which is rapid, sensitive, specific, inexpensive to extend the value for breast cancer prognosis.

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Abbreviations

HRG :

Histidine-rich glycoprotein

AuNPs:

Gold nanoparticles

BC:

Breast cancer

LN:

Lymph node

ER:

Estrogen receptor

PR:

Progesterone receptor

Her-2 neu:

Human epidermal growth factor receptor 2

MMP:

Modified magnet particles

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Acknowledgments

This work was supported by the Egyptian Academy of Research and Technology, the Science and Technology Center, Project 21, and a grant from YJ-STRC to H. Azzazy. The authors acknowledge Mr. Kamel Eid from YJ-STRC for preparing the magnetic nanoparticles.

Conflict of interest

The authors declare that they have no competing interests.

Author information

Authors and Affiliations

  1. Oncology Diagnostic Unit, Medical Biochemistry and Molecular biology Department, Faculty of Medicine, Ain Shams University, P.O. Box 11381, Abbassia, Cairo, Egypt

    Sanaa Eissa, Marwa Matboli & Hebatallah Said

  2. Department of Chemistry and Youssef Jameel Science and Technology Research Center, American University in Cairo, P.O. Box 74, New Cairo, 11835, Egypt

    Hassan M. E. Azzazy & Sherif M. Shawky

  3. General Surgery Department, Faculty of Medicine, Ain Shams University, P.O. Box 11381, Abbassia, Cairo, Egypt

    Fatin A. Anous

Authors
  1. Sanaa Eissa
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  2. Hassan M. E. Azzazy
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  3. Marwa Matboli
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  4. Sherif M. Shawky
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  5. Hebatallah Said
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  6. Fatin A. Anous
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Correspondence to Sanaa Eissa.

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Eissa, S., Azzazy, H.M.E., Matboli, M. et al. The Prognostic Value of Histidine-Rich Glycoprotein RNA in Breast Tissue Using Unmodified Gold Nanoparticles Assay. Appl Biochem Biotechnol 174, 751–761 (2014). https://doi.org/10.1007/s12010-014-1085-x

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  • DOI: https://doi.org/10.1007/s12010-014-1085-x

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