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Small Molecules Differentiate Definitive Endoderm from Human Induced Pluripotent Stem Cells on PCL Scaffold

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Abstract

Human induced pluripotent stem cells (hiPSCs) are attractive sources of cells for disease modeling in vitro, and they may eventually provide access to cells/tissues for the treatment of many degenerative diseases. Stepwise differentiation from hiPSCs to definitive endoderm (DE) will identify a key step in hepatocytes and beta cell development and may prove useful for transplantation therapy for liver diseases and diabetes. Inducer of definitive endoderm 1 (IDE1) is known to play an important role in the regional specification of DE. Here, we have investigated the effect of stimulation with IDE1 on the development of hiPSCs into DE cells in three-dimensional (3D) cultures. The differentiation was determined by immunofluorescence staining with Sox17, FoxA2, and goosecoid (Gsc) and also by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis. In this study, we showed that hiPSCs with 6-day IDE1 treatment (as chemical tool) on poly(ε-caprolactone) (PCL) nanofibrous scaffold were able to differentiate into DE cells.

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Acknowledgments

We thank Tehran University of Medical Sciences for research assistance and the Iranian Council of Stem Cell Technology and Iran National Science Foundation for their supports.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Elham Hoveizi

  2. Department of biosciences and biotechnology, Malek Ashtar University of Technology, Tehran, Iran

    Sirus Khodadadi & Mohammad Ali Nasiri-Khalili

  3. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Shima Tavakol

  4. Department of clinical biochemistry, Faculty of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Oveis Karima

Authors
  1. Elham Hoveizi
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  2. Sirus Khodadadi
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  3. Shima Tavakol
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  5. Mohammad Ali Nasiri-Khalili
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Correspondence to Elham Hoveizi.

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Hoveizi, E., Khodadadi, S., Tavakol, S. et al. Small Molecules Differentiate Definitive Endoderm from Human Induced Pluripotent Stem Cells on PCL Scaffold. Appl Biochem Biotechnol 173, 1727–1736 (2014). https://doi.org/10.1007/s12010-014-0960-9

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  • DOI: https://doi.org/10.1007/s12010-014-0960-9

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