Abstract
Penicillin G acylase (PGA) is a commercially important enzyme that cleaves penicillin G to 6-amino penicillanic acid (6-APA) and phenyl acetic acid (PAA). The strain Bacillus badius has been identified as potential producer of PGA. A detailed calorimetric investigation on PGA production was carried out to enable generation of thermokinetic data possible for commercial application. Reaction calorimetric studies coupled with respirometric studies suggested that enzyme activity of the species B. badius was calorimetrically traceable. Three phases of growth were distinctly noticeable in the metabolic heat-time curve. Increase in enzymatic activity with restricted growth confirmed intracellular nature of the production process. The estimated heat yields due to biomass growth, 10.026 kJ/g, substrate consumption 22.761 kJ/g, and oxygen uptake 383 ± 10 kJ/mol helped to understand the energetic of the organism under study. Low oxycalorific coefficient confirmed the existence of fermentation-coupled metabolism of B. badius.
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Abbreviations
- A :
-
Heat transfer area (m2)
- Q :
-
Heat generated (kJ)
- Q met (t):
-
Cumulative metabolic heat (kJ/L)
- OUR:
-
Oxygen uptake rate (mg/L min)
- q :
-
Heat evolution rate (W)
- q a :
-
Heat flow of the acid or base addition (W)
- q ac :
-
Heat accumulation in the bulk (W)
- q b1 :
-
Baseline heat (W)
- \( {q}_{{\mathrm{CO}}_2} \) :
-
Heat flow of the CO2 vaporization (W)
- q e :
-
Heat flow to the environment through the non-jacketed part of the reactor (W)
- q f :
-
Heat flow of the feed (W)
- q g :
-
Heat flow induced by aeration (W)
- q j :
-
Heat flow through the reactor wall to the jacket oil (W)
- q s :
-
Heat flow due to the stirring power (W)
- q r :
-
Heat generated by the reaction (W)
- T j :
-
Temperature of jacket oil (°C)
- T r :
-
Temperature of reactor contents (°C)
- U :
-
Global heat transfer coefficient (W/m2K)
- Vvm:
-
Volume of air per volume of medium per minute (L/L min)
- Y Q/X :
-
Heat yield coefficient with respect to biomass (kJ/g)
- Y X/S :
-
Biomass yield coefficient with respect to substrate (g/g)
- Y Q/O :
-
Heat generated due to oxygen consumed (kJ/mol)
- Y Q/S :
-
Heat yield due to substrate depletion (kJ/g)
- Y X/O :
-
Biomass yield due to oxygen consumption (g/g mol)
- O:
-
Oxygen
- S:
-
Substrate
- X:
-
Biomass
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Acknowledgements
One of the authors (RK) wishes to acknowledge the Principal of Mepco Schlenk Engineering College for kind permission to publish the paper. The authors wish to express their gratitude to Prof. NR Rajagopal for encouragement.
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Rajendran, K., Sekar, S., Mahadevan, S. et al. Biological Real-Time Reaction Calorimeter Studies for the Production of Penicillin G Acylase from Bacillus badius . Appl Biochem Biotechnol 172, 3736–3747 (2014). https://doi.org/10.1007/s12010-014-0800-y
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DOI: https://doi.org/10.1007/s12010-014-0800-y