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α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro

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Abstract

Streptomyces strain isolated from the soil sediment was studied for its in vitro α-glucosidase and antioxidant properties. Morphological characterization and 16S rRNA partial gene sequencing were carried out to confirm that the strain Loyola AR1 belongs to genus Streptomyces sp. Modified nutrient glucose broth was used as the basal medium for growth and metabolites production. Ethyl acetate extract of Loyola AR1 (EA-Loyola AR1) showed 50 % α-glucosidase inhibition at the concentration of 860.50 ± 2.68 μg/ml. Antioxidant properties such as total phenolic content of EA-Loyola AR1 was 176.83 ± 1.17 mg of catechol equivalents/g extracts. EA-Loyola AR1 showed significant scavenging activity on 2,2-diphenyl-picrylhydrazyl (50 % inhibition (IC50), 750.50 ± 1.61 μg/ml), hydroxyl (IC50, 690.20 ± 2.38 μg/ml), nitric oxide (IC50, 850.50 ± 1.77 μg/ml), and superoxide (IC50, 880.08 ± 1.80 μg/ml) radicals, as well as reducing power. EA-Loyola AR1 showed strong suppressive effect on lipid peroxidation (IC50, 670.50 ± 2.52 μg/ml). Antioxidants of β-carotene linoleate model system reveals significantly lower than butylated hydroxyanisole.

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Acknowledgments

Authors wish to thank the management of the Department of Plant Biology & Plant Biotechnology and M.Sc. Biotechnology Department, Loyola College, Chennai, Tamil Nadu, India, for providing necessary facility to carry out this research work. Also, we thank to Sunil Christhudas (SRF, ICMR, New Delhi), N. Murugan (SRF, Sankara Nethralaya, Chennai), and G. Ramesh Kumar (SRF, Aravind Eye Hospital, Tirunalveli) for their constant support throughout the research work.

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Authors and Affiliations

  1. Department of Plant Biology and Biotechnology, Loyola College, Chennai, 600034, India

    P. Praveen Kumar, J. P. Preetam Raj, I. V. S. Nimal Christhudas, R. Sagaya Jansi, M. Narbert Raj & P. Agastian

  2. Research Department of Plant Biology and Biotechnology, School of Life Science, Loyola College, Chennai, 600034, India

    P. Agastian

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  1. P. Praveen Kumar
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Correspondence to P. Agastian.

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Praveen Kumar, P., Preetam Raj, J.P., Nimal Christhudas, I.V.S. et al. α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro. Appl Biochem Biotechnol 172, 1687–1698 (2014). https://doi.org/10.1007/s12010-013-0650-z

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