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Hepatoprotective Effect of β-Chitosan from Gladius of Sepioteuthis lessoniana Against Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

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Abstract

Chitosan has attracted much attention as a biomedical material, owing to its unique biological activities. In this study, hepatoprotective effect of β-chitosan obtained from the gladius of squid Sepioteuthis lessoniana was studied against carbon tetrachloride (CCl4)-induced oxidative stress and liver injury in rats. The rats that received β-chitosan along with the administration of CCl4 showed significantly decreased plasma and tissue alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and total cholesterol, triglyceride (TG) and free fatty acid (FFA) contents, whereas the treatment with β-chitosan alone markedly increased rat hepatic and circulatory superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) and reduced glutathione (GSH) levels and decreased the malondialdehyde level. Histopathological observations recommended the marked hepatoprotective effect of β-chitosan. The CCl4-induced alterations on circulatory and hepatic antioxidant defence system were normalised by β-chitosan, and it could be concluded that the hepatoprotective effect of chitosan may be due to its antioxidant and antilipidemic property. Therefore, β-chitosan could be considered as antihepatotoxic agent.

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Abbreviations

CCl4 :

Carbon tetrachloride

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

TG:

Triglycerides

FFA:

Free fatty acids

SOD:

Superoxide dismutase

GPx:

Glutathione peroxidase

GSH:

Glutathione

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Acknowledgments

Authors are thankful to the Director and Dean, CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, for providing the necessary facilities. Three of the authors (AS, AS and SV) are also thankful to the Ministry of Environment and Forests (MoEnF), New Delhi, for the financial assistance.

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Authors and Affiliations

  1. CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, 608502, India

    Namasivayam Subhapradha, Pasiyappazham Ramasamy, Vairamani Shanmugam & Annaian Shanmugam

  2. Department of Pharmacology, Chettinad Hospital and Research Institute, Kelambakkam, Tamil Nadu, India

    Ramachandran Saravanan

  3. Department of Biophysics, All India Institute of Medical Sciences,, New Delhi, 110029, India

    Alagiri Srinivasan

Authors
  1. Namasivayam Subhapradha
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  2. Ramachandran Saravanan
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  3. Pasiyappazham Ramasamy
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  4. Alagiri Srinivasan
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  5. Vairamani Shanmugam
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  6. Annaian Shanmugam
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Correspondence to Annaian Shanmugam.

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Subhapradha, N., Saravanan, R., Ramasamy, P. et al. Hepatoprotective Effect of β-Chitosan from Gladius of Sepioteuthis lessoniana Against Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats. Appl Biochem Biotechnol 172, 9–20 (2014). https://doi.org/10.1007/s12010-013-0499-1

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