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Parkin Induces Upregulation of 40S Ribosomal Protein SA and Posttranslational Modification of Cytokeratins 8 and 18 in Human Cervical Cancer Cells

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Abstract

Parkin was originally identified as a protein associated with Parkinson's disease. Recently, numerous research studies have suggested that parkin acts as a tumor suppressor. In accordance with these studies, we previously reported that overexpression of parkin in HeLa cells induced growth inhibition. To elucidate possible mechanisms by which parkin may inhibit cell growth, HeLa cells were infected with adenoviruses expressing either the parkin gene or adenovirus alone for 72 h and a total proteomic analysis was performed using 2-D gel electrophoresis followed by LC-MS/MS. We identified three proteins whose expression changed between the two groups: the 40S ribosomal protein SA (RPSA) was downregulated in parkin virus-infected cells, and cytokeratins 8 and 18 exhibited an acid shift in pI value without a change in molecular weight, suggesting that these proteins became phosphorylated in parkin virus-infected cells. The changes in these three proteins were first observed at 60 h postinfection and were most dramatic at 72 h postinfection. Because upregulation of RPSA and dephosphorylation of cytokeratins 8/18 have been linked with tumor progression, these data suggest that parkin may inhibit cell growth, at least in part, by decreasing RPSA expression and inducing phosphorylation of cytokeratin 8/18.

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Acknowledgments

This study was supported by the KIST Gangneung Institute intramural grant (2Z03850) program and the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2010–0024063).

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Authors and Affiliations

  1. Functional Food Center, Korea Institute of Science and Technology (KIST), Gangneung, 210-340, Republic of Korea

    Dae-Geun Song & Cheol-Ho Pan

  2. Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, 220-710, Republic of Korea

    Yoon Suk Kim, Byung Chul Jung & Ki-Jong Rhee

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  1. Dae-Geun Song
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  2. Yoon Suk Kim
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Correspondence to Ki-Jong Rhee or Cheol-Ho Pan.

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Dae-Geun Song and Yoon Suk Kim authors contributed equally to this manuscript.

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Song, DG., Kim, Y.S., Jung, B.C. et al. Parkin Induces Upregulation of 40S Ribosomal Protein SA and Posttranslational Modification of Cytokeratins 8 and 18 in Human Cervical Cancer Cells. Appl Biochem Biotechnol 171, 1630–1638 (2013). https://doi.org/10.1007/s12010-013-0443-4

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