Abstract
Production of l-menthol by bioprocesses attracts increasing attention nowadays. Herein, we attempted to develop a bioresolution process for production of l-menthol through enantioselective hydrolysis of dl-menthyl benzoate using a newly isolated bacterium from soil samples. Among 129 active soil isolates screened rapidly by thin-layer chromatography, an outstanding bacterial strain numbered ECU2040, which was subsequently identified as Acinetobacter species, was finally selected as our target enzyme producer due to its highest activity and the best enantioselectivity toward l-substrate as confirmed by chiral gas chromatography. The catalytic performance of the cell-free extract from Acinetobacter sp. ECU2040 was preliminarily examined, indicating that its optimal pH and temperature for the reaction were 7.5 and 37 °C, respectively. Under the optimal conditions, the enzymatic reaction was performed on a 1-L scale, affording l-menthol in 48 % yield and 71 % ee.
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Thanks to the financial support from the National Natural Science Foundation of China (nos. 31200050 and 21276082), Ministry of Science and Technology, People’s Republic of China (nos. 2011AA02A210 and 2011CB710800), and the Fundamental Research Funds for the Central Universities, Ministry of Education, People’s Republic of China.
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Ngo-Thi, MT., Yin, JG., Pan, J. et al. Enantioselective Hydrolysis of dl-Menthyl Benzoate by Cell-Free Extract of Newly Isolated Acinetobacter sp. ECU2040. Appl Biochem Biotechnol 170, 1974–1981 (2013). https://doi.org/10.1007/s12010-013-0355-3
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DOI: https://doi.org/10.1007/s12010-013-0355-3