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Ionic Liquid-Induced Structural and Activity Changes in Hen Egg White Lysozyme

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Abstract

Lysozyme crystals in the presence of 1-butyl-3-methylimidazolium tetrafluoroborate ([C4mim]BF4), 1-butyl-3-methylimidazolium chloride ([C4mim]Cl), 1-butyl-3-methylimidazolium bromide([C4mim]Br), and 1,3-dimethylimidazolium iodine([dmim]I) were prepared, and the influence of ionic liquids (ILs) on the structure and activity change of lysozyme was investigated. Fourier transform infrared spectroscopy revealed the major secondary structures of α-helix and β-sheet for lysozyme. It was interesting to note that increases of the band near 2,935 and 1,656 cm−1 from Raman spectroscopy are attributed to the unfolding of lysozyme molecules. A shift in amide III from 1,230 to 1,270 cm−1 in adding [dmim]I occurs, indicating a transformation from β-sheet to random coil. With regard to adding [C4mim]BF4, [C4mim]Cl, and [C4mim]Br, α-helix and β-sheet are the predominant structures for lysozyme. The activity study showed that the ILs used brought a positive effect. Especially, [dmim]I leads to a drastic increase in relative activity, and its value reaches 50 %.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (no. 20806053) and China Postdoctoral Science Foundation (no. 200902274).

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  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Le-Ping Dang, Wen-Zhi Fang, Yan Li, Qian Wang, Hua-Zhi Xiao & Zhan-Zhong Wang

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  1. Le-Ping Dang
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  2. Wen-Zhi Fang
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  3. Yan Li
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  5. Hua-Zhi Xiao
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Correspondence to Zhan-Zhong Wang.

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Dang, LP., Fang, WZ., Li, Y. et al. Ionic Liquid-Induced Structural and Activity Changes in Hen Egg White Lysozyme. Appl Biochem Biotechnol 169, 290–300 (2013). https://doi.org/10.1007/s12010-012-9986-z

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  • DOI: https://doi.org/10.1007/s12010-012-9986-z

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