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Recombinant OmpL1 Protein as a Diagnostic Antigen for the Detection of Canine Leptospirosis

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Abstract

Microscopic agglutination test (MAT) is the standard method for the diagnosis of leptospirosis, which is laborious and the interpretation of the results is subjective. The present work describes the use of recombinant-based IgG ELISA for the serodiagnosis of leptospirosis. We used recombinant outer membrane protein OmpL1 as an antigen for conducting IgG enzyme-linked immunosorbent assay (ELISA). A total of 475 canine serum samples were subjected to IgG ELISA; 294 sera were positive to ELISA, while 283 were positive to MAT. All samples that were positive to MAT were positive to ELISA also, however, few samples which were negative to MAT were positive to ELISA, which suggested that recombinant-based IgG ELISA showed 100 % sensitivity when compared to MAT. Thus, this present study showed that recombinant OmpL1-based IgG ELISA appears to be a better alternative to MAT for the diagnosis of leptospirosis and rOmpL1 protein could be used as a potential diagnostic antigen in different assay formats for leptospirosis.

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Acknowledgments

The authors acknowledge the funding agency, Department of Biotechnology, Govt. of India, for its financial support to carry out this work.

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Authors and Affiliations

  1. Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, 600 007, Tamil Nadu, India

    M. Subathra, T M A Senthilkumar & P. Ramadass

  2. Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Andhra Pradesh, India

    M. Subathra

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  1. M. Subathra
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  2. T M A Senthilkumar
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  3. P. Ramadass
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Correspondence to T M A Senthilkumar.

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Subathra, M., Senthilkumar, T.M.A. & Ramadass, P. Recombinant OmpL1 Protein as a Diagnostic Antigen for the Detection of Canine Leptospirosis. Appl Biochem Biotechnol 169, 431–437 (2013). https://doi.org/10.1007/s12010-012-9973-4

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  • DOI: https://doi.org/10.1007/s12010-012-9973-4

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