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Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. Part II: Study of Volvariella volvacea

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Abstract

Volvariella volvacea strains were studied in relation with their ability to produce biomass, lipids and polysaccharides. Firstly, screening of four strains (AMLR 188, 190, 191 and 192) was performed in agar cultures, where the mycelial growth rate of the strains was measured, and in static liquid cultures, where the production of biomass, the biosynthesis of total cellular lipids and the consumption of glucose were monitored. For all strains, biomass production was significant (13–15 g l−1) and total lipid in dry weight (%, w/w) ranged from 3 to 12 %. Afterwards, a detailed kinetic analysis of mycelial biomass, extra- and intra- cellular polysaccharides (EPS, IPS, respectively) as well as lipid production by a V. volvacea selected strain was conducted in submerged static and agitated cultures. Maximum values of 15 g l−1 biomass, ∼1.0 g l−1 EPS and 5.5 g l−1 IPS were recorded. Agitation did not have severe impact on biomass, EPS and IPS production, but it increased total lipid in dry weight quantities. EPS, IPS and lipid in dry weight values decreased with time. Glucose was the major cellular carbohydrate detected. Total fatty acid analysis of cellular lipids was performed for all V. volvacea strains and linoleic acid Δ9,12C18:2 was predominant. Neutral lipids constituted the major fraction of cellular lipids, but their quantity decreased as fermentation proceeded. Phospholipids were the most saturated lipid fraction.

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Authors and Affiliations

  1. Laboratory of Edible Fungi, Institute of Technology of Agricultural Products, National Agricultural Research Foundation (NAGREF), 1 S. Venizelou Str., Lycovrysi, 14123, Athens, Greece

    Panagiota Diamantopoulou, Eleni Katsarou & Antonios Philippoussis

  2. Department of Food Science and Technology, Agricultural University of Athens, 75 Iera Odos, 11855, Athens, Greece

    Seraphim Papanikolaou & Michael Komaitis

  3. Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, 26500, Patras, Greece

    George Aggelis

Authors
  1. Panagiota Diamantopoulou
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  2. Seraphim Papanikolaou
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  3. Eleni Katsarou
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  6. Antonios Philippoussis
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Correspondence to Antonios Philippoussis.

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Diamantopoulou, P., Papanikolaou, S., Katsarou, E. et al. Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. Part II: Study of Volvariella volvacea . Appl Biochem Biotechnol 167, 1890–1906 (2012). https://doi.org/10.1007/s12010-012-9714-8

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