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Modeling of Pretreatment Condition of Extrusion-Pretreated Prairie Cordgrass and Corn Stover with Poly (Oxyethylen)20 Sorbitan Monolaurate

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Abstract

Extrusion processing has shown potential to be used as a pretreatment method for second-generation bioethanol production. Furthermore, surfactants have been shown to reduce enzyme deactivation and increase the efficiency of hydrolysis. Therefore, a sequential pretreatment technique was developed for corn stover (CS) and prairie cordgrass (PCG) in which a single screw extruder was used for the first pretreatment according to a previously optimized condition using 70–180 °C for feed, barrel, and die zones with 65–155 rpm screw speed. The second pretreatment was optimized in this study at 45–55 °C, 1–4 h, 0.15–0.6 g Tween 20/g glucan according to response surface methodology. Optimization of surfactant pretreatment facilitated the estimation of interaction and higher-order effects for major factors involved in surfactant treatment (temperature, time, surfactant loading). Using 8.6 FPU/g glucan cellulase, the optimum conditions found by fitting appropriate quadratic models to the data increased glucose and xylose yield by 27.5 and 33 % for CS and by 21.5 and 27 % for PCG, respectively. Tween 20 concentrations and pretreatment temperature were the most significant factors affecting sugar yield (p value <0.05). Studies of SDS concentration at and beyond critical micelle concentration (5.2–100 mM) demonstrated a decrease in sugar yield compared to control.

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Acknowledgments

Funding support was provided by Sun Grant project titled “Development of pretreatment strategies”. The authors would also like to thank Novozymes Inc. for providing the commercial enzymes. We would also thank Dr. Karunanithy who helped in the extrusion of corn stover and prairie cordgrass.

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Authors and Affiliations

  1. Department of Agricultural and Biosystems Engineering, South Dakota State University, 1400 North Campus Drive, Brookings, SD, 57007, USA

    Anahita Dehkhoda Eckard & Kasiviswanathan Muthukumarappan

  2. Department of Biology and Microbiology, South Dakota State University, 1400 North Campus Drive, Brookings, SD, 57007, USA

    William Gibbons

Authors
  1. Anahita Dehkhoda Eckard
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  2. Kasiviswanathan Muthukumarappan
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  3. William Gibbons
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Correspondence to Anahita Dehkhoda Eckard.

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Eckard, A.D., Muthukumarappan, K. & Gibbons, W. Modeling of Pretreatment Condition of Extrusion-Pretreated Prairie Cordgrass and Corn Stover with Poly (Oxyethylen)20 Sorbitan Monolaurate. Appl Biochem Biotechnol 167, 377–393 (2012). https://doi.org/10.1007/s12010-012-9698-4

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