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A Novel Hatching Enzyme from Starfish Asterias amurensis: Purification, Characterization, and Cleavage Specificity

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Abstract

Hatching enzyme (HE) is of importance to degrade egg membrane to let the larvae be free. HE was purified and characterized from starfish blastula. The specific activity and the purification ratio of the purified HE with 110.9 kDa of molecular weight were 449.62 U/mg and 7.42-fold, respectively. Its optimal pH and temperature for activity were pH 8.0 and 30 °C, respectively. This enzyme was relatively stable in the range of pH 4.0–6.0 and 30–40 °C. This enzyme was inhibited by ethylene diamine tetraacetic acid (EDTA) and ethylene glycol tetraacetic acid, and also done moderately by Leupeptin, tosyl-lysine chloromethyl ketone, tosyl-phenylalanine chloromethyl ketone, and phenyl-methanesulfonyl fluoride. Zn2+ ion activated HE activity strongly and recovered the EDTA-pretreated activity more than did Ca2+, Mg2+, and Cu2+. Based on the results above, the starfish HE was classified as a zinc metallo- and trypsin-like serine protease. The values of Km, Vmax, and Kcat of the starfish HE on dimethyl casein were 0.31 mg/ml, 0.17 U/ml, and 122.70 s−1, respectively, whereas 1.09 mg/ml, 0.12 U/ml, and 771.98 s−1 on type I collagen. Therefore, the starfish HE could be a potential cosmeceutical because of its strong cleavage specificity on type I collagen.

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Acknowledgments

This work was supported by grant no. RTI 05-01-02 from the Regional Technology Innovation Program of the Ministry of Knowledge Economics (MKE), Republic of Korea. Zhi Jiang Li is a recipient of a graduate fellowship provided by Brain Korea (BK21) program sponsored by the Ministry of Education, Science and Technology, Republic of Korea.

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Authors and Affiliations

  1. Department of Food and Engineering, College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163-319, People’s Republic of China

    Zhi Jiang Li

  2. Department of Marine Food Science and Technology, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung, 210-702, Republic of Korea

    Zhi Jiang Li & Sang Moo Kim

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  1. Zhi Jiang Li
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  2. Sang Moo Kim
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Correspondence to Sang Moo Kim.

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Li, Z.J., Kim, S.M. A Novel Hatching Enzyme from Starfish Asterias amurensis: Purification, Characterization, and Cleavage Specificity. Appl Biochem Biotechnol 169, 1386–1396 (2013). https://doi.org/10.1007/s12010-012-0090-1

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