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Rapid Establishment of p-Nitrophenol Biodegradation in Acetate-Fed Aerobic Granular Sludge

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Abstract

The aim of the study was to investigate the acclimation of precultivated acetate-fed aerobic granular sludge to a toxic xenobiotic biodegradation. Establishment of p-nitrophenol (PNP) biodegradation in acetate-fed aerobic granular sludge and concomitant changes in the microstructure and bacterial community were determined. Rapid establishment of PNP utilization was observed in the granular sludge when fed with PNP as the sole carbon source. The specific PNP removal was 36-mg h-1 g-1 granular biomass at an initial PNP concentration of 50 mg L-1. The presence of PNP resulted in significant membrane damage in a subpopulation of the bacterial consortium, as shown by BacLight viability staining. This was coincided with a significant decrease in the culturable bacterial diversity of the granular biomass. PCR-DGGE analysis revealed a shift and decrease in number of bands during the establishment of PNP biodegradation. Scanning electron microscopy showed the dominance of rod-shaped bacteria in the PNP-utilizing microbial granules. Our results suggest that acetate-fed granular sludge could be quickly adapted for PNP biodegradation.

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References

  1. Adav, S. S., Lee, D. J., Show, K. Y., & Tay, J. H. (2008). Biotechnology Advances, 26, 411–423.

    Article  CAS  Google Scholar 

  2. Lee, D. J., Chen, Y. Y., Show, K. Y., Whitely, C. G., & Tay, J. H. (2010). Advances, 28, 919–934.

    CAS  Google Scholar 

  3. Di Iaconi, C., Ramadori, R., Lopez, A., & Passino, R. (2007). Industrial and Engineering Chemistry Research, 46, 6661–6665.

    Article  Google Scholar 

  4. Arrojo, B., Mosquera-Corral, A., Garrido, J. M., & Méndez, R. (2004). Water Research, 38, 3389–3399.

    Article  CAS  Google Scholar 

  5. Jiang, H.L., Tay, J.H., Maszenan, A.M. and Tay, ST. (2004) 70, 6767-6775.

  6. Tay, S. T. L., Moy, B. Y. P., Jiang, H. L., & Tay, J. H. (2005). Journal of Biotechnology, 115, 387–395.

    Article  CAS  Google Scholar 

  7. Nancharaiah, Y. V., Joshi, H. M., Mohan, T. V. K., Venugopalan, V. P., & Narasimhan, S. V. (2006). Current Science, 91, 503–509.

    CAS  Google Scholar 

  8. Nancharaiah, Y. V., Schwarzenbeck, N., Mohan, T. V., Narasimhan, S. V., Wilderer, P. A., & Venugopalan, V. P. (2006). Water Research, 40, 1539–1546.

    Article  CAS  Google Scholar 

  9. Nancharaiah, Y. V., & Venugopalan, V. P. (2011). Chemosphere, 85, 683–688.

    Article  CAS  Google Scholar 

  10. Wang, S. W., Liu, X. W., Zhang, H. Y., Gong, W. X., Sun, X. F., & Gao, B. Y. (2007). Chemosphere, 69, 769–775.

    Article  CAS  Google Scholar 

  11. Zhu, L., Xu, X., Luo, W., Cao, D., & Yang, Y. (2008). Journal of Applied Microbiology, 104, 152–160.

    CAS  Google Scholar 

  12. Nancharaiah, Y. V., Joshi, H. M., Hausner, M., & Venugopalan, V. P. (2008). Chemosphere, 71, 30–35.

    Article  CAS  Google Scholar 

  13. Quan, X. C., Tang, H., Xiong, W. C., & Yang, Z. F. (2010). Journal of Hazardous Materials, 179, 1136–1142.

    Article  CAS  Google Scholar 

  14. Carucci, A., Milia, S., Gioannis, G. D., & Piredda, M. (2009). Journal of Hazardous Materials, 166, 483–490.

    Article  CAS  Google Scholar 

  15. Ghosh, A., Khurana, M., Chauhan, A., Takeo, M., Chakraborti, A. K., & Jain, R. K. (2010). Environmental Science and Technology, 44, 1069–1077.

    Article  CAS  Google Scholar 

  16. Ray, P., Oubelli, M. A., & Loser, C. (1999). Applied Microbiology and Biotechnology, 51, 284–290.

    Article  CAS  Google Scholar 

  17. Tomei, M. C., & Annesini, M. C. (2005). Environmental Science and Technology, 39, 5059–5065.

    Article  CAS  Google Scholar 

  18. Yi, S., Zhuang, W. Q., Wu, B., Tay, S. T. L., & Tay, J. W. (2006). Environmental Science and Technology, 40, 2396–2401.

    Article  CAS  Google Scholar 

  19. Nancharaiah, Y. V., Dodge, C. J., Venugopalan, V. P., Narasimhan, S. V., & Francis, A. J. (2010). Applied and Environmental Microbiology, 76, 2433–2438.

    Article  CAS  Google Scholar 

  20. Boon, N., Top, E. M., Verstraete, W., & Siciliano, S. D. (2003). Applied and Environmental Microbiology, 69, 1511–1520.

    Article  CAS  Google Scholar 

  21. Nancharaiah, Y. V., Wattiau, P., Wuertz, S., Bathe, S., Mohan, S. V., Wilderer, P. A., & Hausner, M. (2003). Applied and Environmental Microbiology, 69, 4846–4852.

    Article  CAS  Google Scholar 

  22. APHA (1998) Standard methods for the examination of water and wastewater, 19th ed. Washington, DC, USA.

  23. Adav, S. S., Chen, M. Y., Lee, D. J., & Ren, N. Q. (2007). Journal of Biotechnology, 96, 844–852.

    CAS  Google Scholar 

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Author notes

  1. Renu Kadali

    Present address: Department of Biotechnology, VIT University, Vellore, India

  2. Naresh Sharma

    Present address: Environmental and Water Resources Division, Indian Institute of Technology, Chennai, 600036, India

Authors and Affiliations

  1. Biofouling & Biofilm Processes Section, Water and Steam Chemistry Division, Bhabha Atomic Research Centre, Kalpakkam, 603102, India

    Venkata Nancharaiah Yarlagadda, Renu Kadali, Naresh Sharma & Venugopalan Vayalam Purath

  2. Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou, 215123, China

    Raju Sekar

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  1. Venkata Nancharaiah Yarlagadda
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  2. Renu Kadali
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  3. Naresh Sharma
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  4. Raju Sekar
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  5. Venugopalan Vayalam Purath
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Correspondence to Venkata Nancharaiah Yarlagadda.

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Yarlagadda, V.N., Kadali, R., Sharma, N. et al. Rapid Establishment of p-Nitrophenol Biodegradation in Acetate-Fed Aerobic Granular Sludge. Appl Biochem Biotechnol 166, 1225–1235 (2012). https://doi.org/10.1007/s12010-011-9509-3

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  • DOI: https://doi.org/10.1007/s12010-011-9509-3

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