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Maximum Saccharification of Cellulose Complex by an Enzyme Cocktail Supplemented with Cellulase from Newly Isolated Aspergillus fumigatus ECU0811

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Abstract

Either the natural biodegradation process or the industrial hydrolytic process requires synergistic interactions between various cellulases. However, it is sometimes impeded by low hydrolytic rate of existing cellulases and the lack of accessory enzymes. Herein, the ability of a commercial cellulase (Spezyme CP, from Genencor) to degrade steam explosion-pretreated corn stover was significantly improved. Firstly, a fungal cellulase producer, Aspergillus fumigatus ECU0811, was isolated from hundreds of soil samples. A 96-deep-well microscale-based platform was developed here to reduce the labor-intensive screening work and proved to be consistent with macroscale screening work. After optimization of fermentation, 3% corn cob could induce A. fumigatus ECU0811 to yield the highest cellulase production. Based on the high activities of β-glucosidase and xylanase by A. fumigatus ECU0811, 0.91 and 125 U/mg protein, respectively, an enzyme cocktail was composed with a fixed dosage of Spezyme CP (CPCel) at 14.2 filter paper units (FPU)/g glucan and varied dosages of A. fumigatus cellulase (AFCel). Consequently, the glucan-to-glucose conversion of corn stover was increased from 25.6% in the presence of CPCel at a dosage of 14.2 FPU/g glucan to 99.5% in the presence of the enzyme cocktail (14.2 FPU CPCel plus 1.21 FPU AFCel per gram of glucan). On the other side, it reduced the total protein amount of CPCel by as much as tenfold, which extremely improved the hydrolytic rate of Spezyme CP and reduced its dosage.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Nos. 20902023 & 31071604), Ministry of Science and Technology, P.R. China (Nos. 2009CB724706), and China National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

    Dan Wang, Jie Sun, Hui-Lei Yu, Chun-Xiu Li, Jie Bao & Jian-He Xu

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  1. Dan Wang
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  2. Jie Sun
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  3. Hui-Lei Yu
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  4. Chun-Xiu Li
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  5. Jie Bao
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  6. Jian-He Xu
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Correspondence to Jian-He Xu.

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Dan Wang and Jie Sun contributed equally to this work.

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Wang, D., Sun, J., Yu, HL. et al. Maximum Saccharification of Cellulose Complex by an Enzyme Cocktail Supplemented with Cellulase from Newly Isolated Aspergillus fumigatus ECU0811. Appl Biochem Biotechnol 166, 176–186 (2012). https://doi.org/10.1007/s12010-011-9414-9

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  • DOI: https://doi.org/10.1007/s12010-011-9414-9

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