Abstract
A crude preparation of horseradish roots was used as a low-purity source of horseradish peroxidase (HRP) in dye decolorization experiments. The technical feasibility of the process was studied in bench scale for enzymatic removal of acid orange 7 (AO7), a synthetic dye. Further studies were carried out to understand the effects of process parameters such as pH value, H2O2 level, concentrations of the synthetic dye, and HRP during enzyme-mediated dye degradation. Experimental data revealed that the concentration of AO7, pH of the aqueous phase, amount of the enzyme, and H2O2 level played significant roles on the overall enzymatic reaction. Polyethylene glycol, as an anti-inactivation of HRP, in various concentrations showed no significant effect on the decolorization. The experimental data of initial reaction rates were fitted using an analytical equation proposed by Michaelis–Menten. The acute toxicity tests using Daphnia magna exhibited that the enzymatic treatment significantly decreased the toxicity of the dye solution.
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This work as a part of PhD thesis project in Tehran University of Medical Science was supported by the grants from the Iran National Science Foundation (INSF).
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Gholami-Borujeni, F., Mahvi, A.H., Nasseri, S. et al. Enzymatic Treatment and Detoxification of Acid Orange 7 from Textile Wastewater. Appl Biochem Biotechnol 165, 1274–1284 (2011). https://doi.org/10.1007/s12010-011-9345-5
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DOI: https://doi.org/10.1007/s12010-011-9345-5