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Expression of MMP2 and MMP9 (Gelatinases A and B) in Human Colon Cancer Cells

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Abstract

Matrix metalloproteinases (MMPs) are a family of zinc-dependent neutral endopeptidases, collectively capable of degrading essentially all matrix components. Elevated levels of distinct MMPs are detected in tumor tissue or serum of patients with advanced cancer, and they are the major prognostic indicators in cancer. Inhibition of MMPs has been explored as a therapeutic goal for almost two decades. Nitric oxide (NO), a free radical plays an important role in signaling pathways in regulation of MMP expression. In the present study, we demonstrated the role of exogenous NO levels in the regulation of MMP2 and MMP9 (gelatinases A and B) in colon cancer cell line WiDr and its inhibition with emodin (a naturally occurring anthraquinone).

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Abbreviations

MMP:

Matrix metalloproteinases

NO:

Nitric oxide

ECM:

Extracellular matrix

NOs:

Nitric oxide synthases

DMEM:

Dulbecco’s modified eagle medium

PBS:

Phosphate-buffered saline

EDTA:

Ethylenediaminetetra acetate

SNP:

Sodium nitroprusside

MTT:

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

DMSO:

Dimethyl sulfoxide

ELISA:

Enzyme-linked immunosorbent assay

RT-PCR:

Reverse transcriptase-PCR

MoMLV:

Molony murine leukemia virus

TAE:

Tris acetate-EDTA buffer

MMPI:

Matrix metalloproteinases inhibitors

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Acknowledgement

The authors thank the Management of Karpagam University and G Srinivas Scientist C, Department of Biochemistry of Sree Chitra Tirunal Institute for Medical Sciences and Technology for providing lab facilities and constant encouragement for this research work.

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Authors and Affiliations

  1. Department of Biochemistry, Karpagam Arts and Science College, Coimbatore, 641 029, India

    Umadevi Damodharan

  2. Department of Biochemistry, Karpagam University, Coimbatore, 641 029, India

    Ravikumar Ganesan & Uma C. Radhakrishnan

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  1. Umadevi Damodharan
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  2. Ravikumar Ganesan
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  3. Uma C. Radhakrishnan
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Correspondence to Uma C. Radhakrishnan.

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Damodharan, U., Ganesan, R. & Radhakrishnan, U.C. Expression of MMP2 and MMP9 (Gelatinases A and B) in Human Colon Cancer Cells. Appl Biochem Biotechnol 165, 1245–1252 (2011). https://doi.org/10.1007/s12010-011-9342-8

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  • DOI: https://doi.org/10.1007/s12010-011-9342-8

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