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Analysis of the Amino Acids of Soy Globulins by AOT Reverse Micelles and Aqueous Buffer

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Abstract

The 7S and 11S globulins from soybean proteins using reverse micelle and aqueous buffer extraction methods were characterized by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscope (SEM), and their amino acid compositions were also evaluated. SDS-PAGE did not show electrophoretic differences between 7S and 11S globulin subunits with two extraction methods. SEM analysis showed that the AOT reverse micelle processing of 7S and 11S globulins induced a reduction of droplet size. Some individual amino acid contents of 7S and 11S globulins using two extraction methods were different, some were similar. In all the samples, the glutamic acid, aspartic acid, and leucine were the dominant amino acids while the cystine and methionine were the first-limiting amino acids. The proportion of essential amino acids to the total amino acids (E/T) of the 7S globulin from aqueous buffer and reverse micelles was similar. While significant differences were obtained in the proportion of E/T of the 11S globulin.

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Acknowledgments

Financial support of this work came from the Promotive Research Fund for Excellent Young and Middle-Aged Scientists of Shandong Province of China (grant no. BS2010NY027) and Project of Agriculture Scientific and Technological Achievement Transfer of China (grant no. 2009GB2C600198).

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Authors and Affiliations

  1. Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, No. 198, Gongyebei Road, Jinan, 250100, China

    Xiaoyan Zhao, Jun Chen, Xiangqing Ling, Peng Deng, Qingjun Zhu & Fangling Du

  2. Department of Orthopedics, Second Affiliated Hospital of Southeast University, Nanjing, 210003, China

    Zhifang Lu

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  1. Xiaoyan Zhao
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  2. Jun Chen
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Correspondence to Xiaoyan Zhao or Fangling Du.

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Zhao, X., Chen, J., Lu, Z. et al. Analysis of the Amino Acids of Soy Globulins by AOT Reverse Micelles and Aqueous Buffer. Appl Biochem Biotechnol 165, 802–813 (2011). https://doi.org/10.1007/s12010-011-9298-8

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  • DOI: https://doi.org/10.1007/s12010-011-9298-8

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