Skip to main content

Advertisement

SpringerLink
Log in

Expression and Production of Therapeutic Recombinant Human Platelet-Derived Growth Factor-BB in Pleurotus eryngii

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Recombinant human platelet-derived growth factor-BB (rhPDGF-BB) is widely used in many therapeutic applications. Until now, there has been no report on rhPDGF-BB expressed in fungi. In this study, we tested whether Pleurotus eryngii could support the expression of human therapeutic rhPDGF-BB protein. A binary vector pCAMBIA1304 containing the hPDGF-BB gene was constructed and introduced into P. eryngii via Agrobacterium tumefaciens-mediated transformation. The transformation of hPDGF-BB gene was confirmed by Southern blot and PCR, whereas the expression was confirmed by Western blot analysis. The recombinant hPDGF-BB reached a maximum expression level of 1.98% of total soluble protein in transgenic mycelia and was in dimeric form. A bioassay revealed that hPDGF-BB expressed in P. eryngii increased proliferation of NIH-3T3 cells similarly to standard material. These results suggest that P. eryngii can be a robust system for the production of human therapeutic proteins including the hPDGF-BB.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Habenicht, A. J., Salbach, P., Janssen-Timmen, U., Blattner, C., & Schettler, G. (1992). Klinische Wochenschrift, 8, 53–59.

    Google Scholar 

  2. Kallianinen, L. K., Hirshberg, J., Marchant, B., & Rees, R. S. (2000). Plastic and Reconstructive Surgery, 6, 1243–1248.

    Article  Google Scholar 

  3. Li, W. L., Yamada, Y., Ueno, M., Nishikawa, S., Nishikawa, S. I., & Takakura, N. (2006). Journal of Biochemistry, 140, 267–273.

    Article  CAS  Google Scholar 

  4. Board, R., & Jayson, G. C. (2005). Drug Resistance Updates: Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy, 8, 75–83.

    CAS  Google Scholar 

  5. Frederiksson, L., Li, H., & Eriksson, U. (2004). Cytokine & Growth Factor Reviews, 15, 197–204.

    Article  Google Scholar 

  6. Senet, P. (2004). Becaplermin gel (Regranex gel). Annales de Dermatologie et de Vénéréologie, 131, 351–358.

    Article  CAS  Google Scholar 

  7. Wieman, T. J. (1997). American Journal of Surgery, 176, 74S–79S.

    Article  Google Scholar 

  8. Steed, D. L. (1995). Journal of Vascular Surgery, 1, 71–81.

    Article  Google Scholar 

  9. Mandracchia, V. J., Sanders, S. M., & Frerichs, J. A. (2001). Clinics in Podiatric Medicine and Surgery, 18, 189–209.

    CAS  Google Scholar 

  10. Harrison-Balestra, C., Eaglstein, W. H., Falabela, A. F., & Kirsner, R. S. (2002). Dermatologic Surgery, 28, 755–759.

    Article  Google Scholar 

  11. Hom, D. B., & Manivel, J. C. (2003). The Laryngoscope, 113, 1566–1571.

    Article  Google Scholar 

  12. Hollinger, J. O., Hart, C. E., Hirsch, S. N., Lynch, S., & Friedlaender, G. E. (2007). The Journal of Bone and Joint Surgery. American Volume, 90, 48–54.

    Article  Google Scholar 

  13. Pahl, H. L. (1999). Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene, 18, 6853–6866.

    Article  CAS  Google Scholar 

  14. Peng, F., Dhillon, N. K., Yao, H., Zhu, X., Williams, R., & Buch, S. (2008). The European Journal of Neuroscience, 7, 1255–1264.

    Article  Google Scholar 

  15. van Steensel, L., Paridaens, D., Dingjan, G. M., van Daele, P. L., van Hagen, P. M., Kuijpers, R. W., et al. (2010). Investigative Ophthalmology & Visual Science, 2, 1002–1007.

    Article  Google Scholar 

  16. Hoppe, J., Weich, H. A., & Eichner, W. (1989). Biochemistry, 28, 2956–2960.

    Article  CAS  Google Scholar 

  17. Alexander, D. M., Hesson, T., Mannarino, A., Cable, M., & Dalie, B. L. (1992). Protein Expression and Purification, 3, 204–211.

    Article  CAS  Google Scholar 

  18. Karumuri, N. N., Gangireddy, S. R., Narala, V. R., Majee, S. S., Gunwar, S., & Reddy, R. C. (2007). Biotechnology Letters, 29, 1333–1339.

    Article  CAS  Google Scholar 

  19. Ostman, A., Rall, L., Hammacher, A., Wormstead, M. A., Coti, D., Valenzuela, P., et al. (1988). The Journal of Biological Chemistry, 31, 16202–16208.

    Google Scholar 

  20. Wang, Y., Xue, L., Li, Y., Zhu, Y., Yang, B., & Wang, X. (2009). Prikladnaia Biokhimiia i Mikrobiologiia, 2, 176–180.

    Google Scholar 

  21. Giese, N., May-Siroff, M., LaRochelle, W. J., van Wyke Coelingh, K., & Aaronson, S. A. (1989). Journal of Virology, 7, 3080–3086.

    Google Scholar 

  22. Nevalainen, K. M., Teo, V. S., & Bergquist, P. L. (2005). Trends in Biotechnology, 23, 468–474.

    Article  CAS  Google Scholar 

  23. Fernandez, J. M., & Hoeffler, J. P. (1999). Gene expression systems. San Diego: Academic.

    Google Scholar 

  24. Demain, A. L., & Vaishnav, P. (2009). Biotechnology Advances, 27, 297–306.

    Article  CAS  Google Scholar 

  25. Berends, E., Scholtmeijer, K., Wosten, H. A., Bosch, D., & Lugones, L. G. (2009). Trends in Microbiology, 10, 439–443.

    Article  Google Scholar 

  26. Rodriguez Estrada, A. E., & Royse, D. J. (2007). Bioresource Technology, 98, 1898–1906.

    Article  CAS  Google Scholar 

  27. Royse, D. J. (1999). Mushroom News, 47, 4–8.

    Google Scholar 

  28. Holsters, M., De Waele, D., Depicker, A., Messens, E., Van Montague, M., & Schell, J. (1978). Molecular & General Genetics: MGG, 163, 168–181.

    Google Scholar 

  29. Scott, O. R., & Bendich, A. J. (1997). Extraction of total cellular DNA from plant, algae and fungi. In S. B. Gelvin & R. A. Schilperoort (Eds.), Plant molecular biology manual, D1 (pp. 1–8). Dordrecht: Kluwer Academic.

    Google Scholar 

  30. Laemmli, U. K. (1970). Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  31. Apte-Deshpande, A., Rewanwar, S., Kotwal, P., Raiker, V. A., & Padmanabhan, S. (2009). Biotechnology and Applied Biochemistry, 54, 197–205.

    Article  CAS  Google Scholar 

  32. Calderon-cacia, M., Tekamp-Olson, P., Allen, J., & George-Nascimento, C. (1992). Biochemical and Biophysical Research Communications, 187, 1193–1199.

    Article  CAS  Google Scholar 

  33. Punt, P. J., van Biezen, N., Conesa, A., Albers, A., Mangnus, J., & van den Hondel, C. (2002). Trends in Biotechnology, 5, 200–206.

    Article  Google Scholar 

  34. Gasser, B., & Mattanovich, D. (2007). Biotechnology Letters, 29, 201–212.

    Article  CAS  Google Scholar 

  35. De Groot, M. J. A., Bundock, P., Hooykaas, P. J. J., & Beijersbergen, A. G. M. (1998). Nature Biotechnology, 16, 839–842.

    Article  Google Scholar 

  36. Chen, X., Stone, M., Schlagnhaufer, C., & Romaine, C. P. (2000). Applied and Environmental Microbiology, 664, 510–513.

    Google Scholar 

  37. Michielse, C. B., Hookaas, P. J., van den Hondel, C. A., & Ram, A. F. (2005). Current Genetics, 48, 1–17.

    Article  CAS  Google Scholar 

  38. Wroblewski, T., Tomczakt, A., & Michelmore, R. (2005). Plant Biotechnology Journal, 3, 259–723.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by Technology Development Program for Agriculture and Forestry (20080329), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea

Author information

Authors and Affiliations

  1. Department of Biotechnology, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju, 501-759, Republic of Korea

    Jun-Hui Choi, Kumar Sapkota, Se-Eun Park & Sung-Jun Kim

  2. Department of Alternative Medicine, Gwangju University, Gwangju, 503-703, Republic of Korea

    Seung Kim

  3. Central Department of Zoology, Tribhuvan University, Kirtipur, Kathmandu, Nepal

    Kumar Sapkota

Authors
  1. Jun-Hui Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kumar Sapkota
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Se-Eun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sung-Jun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sung-Jun Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Choi, JH., Kim, S., Sapkota, K. et al. Expression and Production of Therapeutic Recombinant Human Platelet-Derived Growth Factor-BB in Pleurotus eryngii . Appl Biochem Biotechnol 165, 611–623 (2011). https://doi.org/10.1007/s12010-011-9279-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-011-9279-y

Keywords

Search

Navigation