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Cellulase Production by Streptomyces viridobrunneus SCPE-09 Using Lignocellulosic Biomass as Inducer Substrate

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Abstract

An actinomycete strain, isolated from a soil sample under a sugar cane plantation in Brazil and identified as Streptomyces viridobrunneus SCPE-09, was selected as a promising cellulolytic strain, and tested for its ability to produce cellulases from agro-industrial residues. Sugar cane bagasse or wheat bran was tested as carbon source, and corn steep liquor tested as nitrogen source. Different concentrations of carbon and nitrogen were tested using factorial design to identify optimal cellulose production. The results showed that media containing wheat bran 2.0% (w/v) and corn steep liquid 0.19% (w/v) lead to the highest production, 2.0 U mL−1 of CMCase, obtained on the fifth day of fermentation. The pH and temperature profile showed optimal activity at pH 4.9 and 50°C. As for thermostability, endoglucanases were most tolerant at 50°C, retaining more than 80% of maximal activity even after 2 h of incubation. Zymogram analyses using supernatant from growth under optimized conditions revealed the presence of two CMCase bands with apparent molecular masses of 37 and 119 kDa. The combination of pH tolerance and CMCase production from agro-industrial residues by S. viridobrunneus SCPE-09 offers promise for future bioethanol biotechnologies.

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Acknowledgments

This work was supported by Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (MCT/CNPq), Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES), Financiadora de Estudos e Projetos (FINEP), and Conselho de Ensino para Graduados e Pesquisas (CEPG/UFRJ).

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Authors and Affiliations

  1. Dep. Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, CCS, Universidade Federal do Rio de Janeiro (UFRJ), CEP: 21941-590, Rio de Janeiro, RJ, Brazil

    Fábio Nuno Marques Da Vinha, Mônica Pires Gravina-Oliveira, Marcella Novaes Franco, Andrew Macrae & Rosalie Reed Rodrigues Coelho

  2. Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia (UFRB), CEP: 44380-000, Cruz das Almas, BA, Brazil

    Rodrigo Pires Nascimento

  3. Instituto de Química, CT, Universidade Federal do Rio de Janeiro (UFRJ), CEP: 21941-590, Rio de Janeiro, RJ, Brazil

    Elba Pinto da Silva Bon

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  1. Fábio Nuno Marques Da Vinha
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Correspondence to Rodrigo Pires Nascimento.

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Da Vinha, F.N.M., Gravina-Oliveira, M.P., Franco, M.N. et al. Cellulase Production by Streptomyces viridobrunneus SCPE-09 Using Lignocellulosic Biomass as Inducer Substrate. Appl Biochem Biotechnol 164, 256–267 (2011). https://doi.org/10.1007/s12010-010-9132-8

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