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Inhibitory Effects of Fatty Acids on the Activity of Mushroom Tyrosinase

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Abstract

The effects of fatty acids, octanoic acid, (2E, 4E)-hexa-2,4-dienoic acid, hexanoic acid, (2E)-but-2-enoic acid, and butyric acid on the activities of mushroom tyrosinase have been investigated. The results showed that the fatty acids can potently inhibit both monophenolase activity and diphenolase activity of tyrosinase, and that the unsaturated fatty acids exhibited stronger inhibitory effect against tyrosinase than the corresponding saturated fatty acids, and the inhibitory effects were enhanced with the extendability of the fatty acid chain. For the monophenolase activity, the fatty acids could not only lengthen the lag period, but also decrease the steady-state activities. For the diphenolase activity, fatty acids displayed reversible inhibition. Kinetic analyses showed that octanoic acid and hexanoic acid were mixed-type inhibitors and (2E,4E)-hexa-2,4-dienoic acid and (2E)-but-2-enoic acid were noncompetitive inhibitors. The inhibition constants have been determined and compared.

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Acknowledgments

The present investigation was supported by the National Key Technology R & D Program of China (No. 2007BAD07B06), Grant 30570408 of the Natural Science Foundation of China, and by Grant 2004N002 and Grant 2007N0051 of the Science and Technology Foundation of Fujian Province.

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, 361005, China

    Yun-Ji Guo, Zhi-Zhen Pan, Yong-Hua Hu, Feng-Jiao Liu, Yan Shi & Qing-Xi Chen

  2. College of Urban and Environmental Sciences, Peking University, Peking, 100871, China

    Chao-Qi Chen

  3. Cancer Research Center of Medical College, Xiamen University, Xiamen, 361005, China

    Jiang-Hua Yan

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  1. Yun-Ji Guo
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  2. Zhi-Zhen Pan
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  4. Yong-Hua Hu
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  5. Feng-Jiao Liu
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  6. Yan Shi
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  7. Jiang-Hua Yan
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  8. Qing-Xi Chen
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Correspondence to Jiang-Hua Yan or Qing-Xi Chen.

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Yun-Ji Guo and Zhi-Zhen Pan contributed equally to this work.

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Guo, YJ., Pan, ZZ., Chen, CQ. et al. Inhibitory Effects of Fatty Acids on the Activity of Mushroom Tyrosinase. Appl Biochem Biotechnol 162, 1564–1573 (2010). https://doi.org/10.1007/s12010-010-8938-8

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  • DOI: https://doi.org/10.1007/s12010-010-8938-8

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