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Magnetic Screening of the Potential Targeted Protein of Salvianolic Acid B Using T7 Phage Display Library

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Abstract

Salvianolic acid B is one of the effective components from the Chinese traditional drug Salvia miltiorrhiza (Danshen), which is widely used as a usual clinic drug for atherosclerosis-related disorder patients in China. But the targeting protein of salvianolic acid B is still not known. The possible targeting proteins of salvianolic acid B were explored by high throughput screening in this paper. Attached to the magnetic nanoparticles, salvianolic acid B was used for screening the high-affinity protein from the displaying cDNA peptide library phage. After biopanning, the selected protein or peptide sequences were used to explore the whole proteins containing the selected sequences in the National Center for Biotechnology Information website using blast. One of the selected phages was carried out by affinity analysis with salvianolic acid B using capillary electrophoresis (CE). The CE results indicated that the protein or peptide on the surface of the selected phages could bind the drug salvianolic acid B. The results are helpful to preliminarily explain the pharmacology of salvianolic acid B.

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Authors and Affiliations

  1. Institute of Biomedical Engineering, Shanghai University, Shanghai, 200072, People’s Republic of China

    Shupeng Liu

  2. Department of Biomedical Engineering, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Shupeng Liu

  3. Department of Biomedical Engineering, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Ye Sun

  4. Shanghai Information Center for Life Sciences, Shanghai Institutes of Biological Sciences (SIBS), The Chinese Academy of Sciences (CAS), Shanghai, 200031, People’s Republic of China

    Heng Chen

  5. School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Suxian Song & Yuhong Xu

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  1. Shupeng Liu
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  2. Ye Sun
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  3. Heng Chen
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  4. Suxian Song
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  5. Yuhong Xu
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Correspondence to Shupeng Liu.

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Liu, S., Sun, Y., Chen, H. et al. Magnetic Screening of the Potential Targeted Protein of Salvianolic Acid B Using T7 Phage Display Library. Appl Biochem Biotechnol 162, 1206–1213 (2010). https://doi.org/10.1007/s12010-009-8888-1

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  • DOI: https://doi.org/10.1007/s12010-009-8888-1

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