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Increase of Docosahexaenoic Acid Production by Schizochytrium sp. Through Mutagenesis and Enzyme Assay

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Abstract

The present study focused on improving docosahexaenoic acid (DHA) production by Schizochytrium sp. through N-methyl-N-nitro-N-nitrisiguanidine treatment coupled with ultraviolet radiation based on the metabolic pathway analysis. The activity of glucose-6-phosphate dehydrogenase of the mutant was higher than the parent strain, which indicated that the hexose monophosphate pathway of the mutant was strengthened, and more NADPH was thus produced. Also, the activities of malic enzyme and ATP–citrate lyase in the cell extract of the mutant were higher than the parent strain, which indicated that the screening method increased NADPH and acetyl–CoA supply in vivo effectively. Finally, in the batch culturing of the mutant, 34.84% higher lipid was accumulated with the cell dry weight at the same level compared with the parent strain. Moreover, the DHA percentage of the total fatty acids up to 56.22% was achieved using the mutant, which was 38.88% higher than the parent strain. When the cultures were maintained under appropriate conditions, the final DHA yield was 0.20 and 0.11 g/g dry biomass, for the mutant and parent, respectively.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (no. 20606018), the Key Program of National Natural Science Foundation of China (no. 20936002), the National Basic Research Program of China (no. 2007CB707805), the Scientific Research Project for Post-graduate in Jiangsu Province of China (no. CX07s_032z), and the Fifth of Six Projects Sponsoring Talent Summits of Jiangsu Province.

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  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People’s Republic of China

    Min Lian, He Huang, Lujing Ren, Xiaojun Ji, Jingyao Zhu & Lijing Jin

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  1. Min Lian
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  2. He Huang
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  3. Lujing Ren
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  4. Xiaojun Ji
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Correspondence to He Huang.

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Lian, M., Huang, H., Ren, L. et al. Increase of Docosahexaenoic Acid Production by Schizochytrium sp. Through Mutagenesis and Enzyme Assay. Appl Biochem Biotechnol 162, 935–941 (2010). https://doi.org/10.1007/s12010-009-8865-8

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