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Snail Hepatopancreatic Lipase: A New Member of Invertebrates Lipases' Group

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Abstract

Higher animal's lipases are well characterized; however, much less is known about lipases from mollusks. A lipolytic activity was located in the land snail (Eobania vermiculata) digestive glands (hepatopancreas), from which a snail digestive lipase (SnDL) was purified. Pure SnDL has a molecular mass of 60 kDa; it does not present the interfacial activation phenomenon. It was found to be more active on short-chain triacylglycerols than on long-chain triacylglycerols. The NH2-terminal sequence of the SnDL shows 66% of identity with the 17 NH2-terminal amino acids of a putative lipase from sea urchin (Strongylocentrotus purpuratus). No sequence identity was found with known lipases. Interestingly, neither colipase nor bile salts were detected in the snail hepatopancreas. This suggests that colipase evolved in vertebrates simultaneously with the appearance of an exocrine pancreas and a true liver which produces bile salts. Altogether, these results suggest that SnDL is a member of a new group of digestive lipases belonging to invertebrates.

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Abbreviations

AG:

Arabic gum

BSA:

Bovine serum albumin

DEAE:

Diethylaminoethyl

FPLC:

Fast protein liquid chromatography

NaDC:

Sodium deoxycholate

NaTDC:

Sodium taurodeoxycholate

SDS:

Sodium dodecyl sulfate

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

SnDL:

Snail digestive lipase

PVDF:

Polyvinylidene difluoride

TC3 :

Tripropionin

TC4 :

Tributyrin

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Acknowledgments

The authors would like to thank Pr. Hafedh Mejdoub and Dr. Abir Ben Bacha for their kind help with NH2-terminal sequencing. This work received financial support from DGRST (Tunisia) granted to “Laboratoire de Biochimie et de Génie Enzymatique des Lipases”.

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Authors and Affiliations

  1. Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de Soukra, BPW 3038, Sfax, Tunisia

    Sawsan Amara, Ahmed Fendri, Nadia Ben Salem, Youssef Gargouri & Nabil Miled

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  1. Sawsan Amara
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  2. Ahmed Fendri
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  3. Nadia Ben Salem
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  4. Youssef Gargouri
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  5. Nabil Miled
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Correspondence to Nabil Miled.

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Amara, S., Fendri, A., Ben Salem, N. et al. Snail Hepatopancreatic Lipase: A New Member of Invertebrates Lipases' Group. Appl Biochem Biotechnol 162, 942–952 (2010). https://doi.org/10.1007/s12010-009-8837-z

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  • DOI: https://doi.org/10.1007/s12010-009-8837-z

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