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Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

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Abstract

This work aimed to immobilize the antibody anti-galectin-3 onto polysiloxane–polyvinyl alcohol (POS-PVA) support, to evaluate its capacity to capture the serum antigen galectin-3 and to quantify by ELISA the antigen levels in sera from patients with prostatic adenocarcinoma (PA) and benign prostatic hyperplasia (BPH) and healthy individuals. Also, for comparative effect, the galectin-3 expression in the prostate tissue through immunohistochemistry was evaluated. The optical density (galectin-3 level) values established for the sera from PA and BPH patients were lower compared with those found for the healthy individuals. Galectin-3 immunohistochemically showed a significant increase and reduction of the cytoplasmatic protein expression in BPH and PA, respectively, compared with the normal prostate. These results showed that POS-PVA disks could be used as solid phase to immobilize serum galectins and in immunoassays procedures for the correspondent IgG anti-galectins detection in human sera.

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Authors and Affiliations

  1. Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, Brazil

    Mario Ribeiro de Melo-Júnior, Jorge Luiz Silva Araújo-Filho, Nicodemos Teles de Pontes-Filho & Luiz Bezerra de Carvalho Jr

  2. Associação Caruaruense de Ensino Superior (ASCES), Recife, Brazil

    Mario Ribeiro de Melo-Júnior

  3. Hospital Oswaldo Cruz, Universidade de Pernambuco, UPE, Recife, Brazil

    Consuelo Antunes Barreto Lins

  4. Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brazil

    Luiz Bezerra de Carvalho Jr

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  1. Mario Ribeiro de Melo-Júnior
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  2. Jorge Luiz Silva Araújo-Filho
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Correspondence to Mario Ribeiro de Melo-Júnior.

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de Melo-Júnior, M.R., Araújo-Filho, J.L.S., Lins, C.A.B. et al. Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis. Appl Biochem Biotechnol 160, 2198–2207 (2010). https://doi.org/10.1007/s12010-009-8753-2

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