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d-glucose Enhanced 5-Aminolevulinic Acid Production in Recombinant Escherichia coli Culture

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Abstract

In this study, we introduced a new strategy, feeding d-glucose, to overproduce extracellular 5-aminolevulinic acid (ALA) in the recombinant Escherichia coli. We investigated that the d-glucose concentration is dependent on extracellular ALA production. The results indicated that increasing d-glucose concentration in bacteria culture enhanced final cell density and ALA yield and simultaneously decreased the activities of ALA synthase (ALAS) and ALA dehydratase (ALAD); then, the inhibitory effect of d-glucose on ALAS activity was relieved with the metabolism of d-glucose. when 4.0 g/L d-glucose was added at late exponential phase; 1.46 g/L ALA was achieved in shaking culture, which is 47% or 109% higher than the ALA yields with 30 mM levulinic acid of ALAD inhibitor or no inhibitor. In jar fermenter, final extracellular ALA concentration reached 3.1 g/L by feeding with d-glucose.

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Acknowledgments

We gratefully acknowledge Meng, F. J., Ph.D., for revising the manuscript. This study was supported by the Scientific Research Fund of the Zhejiang Provincial Education Department (no. 20070617).

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Authors and Affiliations

  1. School of Biology and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China

    Xiao Xia Liu, Yu Jie Wang & Li Ling Cai

  2. College of Life Science, Henan Normal University, Xinxiang, 453007, China

    Lan Wang

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  1. Xiao Xia Liu
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Correspondence to Xiao Xia Liu.

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Liu, X.X., Wang, L., Wang, Y.J. et al. d-glucose Enhanced 5-Aminolevulinic Acid Production in Recombinant Escherichia coli Culture. Appl Biochem Biotechnol 160, 822–830 (2010). https://doi.org/10.1007/s12010-009-8608-x

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