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In Vitro Osteoblast Differentiation is Negatively Regulated by Hoxc8

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Abstract

Hoxc8 has multiple roles in normal skeletal development. In this paper, a MC3T3-E1 subclone 4 osteogenic cell differentiation model was used to examine expression of Hoxc8 at multiple stages of osteogenesis. We found that Hoxc8 expression levels do not change in the early stage but increase in the middle stage and decrease in the late stage of osteogenesis. A knockdown of Hoxc8 by small-interfering RNA transfection in C2C12 cells indicated that Hoxc8 is a negative regulator of osteogenesis. Similarly, expression of Hoxc8 in C2C12 cells decreases alkaline phosphatase levels induced by bone morphogenetic protein-2 (BMP-2). The results of this study showed that Hoxc8 is involved in BMP-2-induced osteogenesis, and osteoblast differentiation in vitro is negatively regulated by Hoxc8, suggesting that Hoxc8 regulation is essential for osteoblast differentiation.

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Acknowledgments

This work was primarily supported by grant no. KRF-2005-204-C00074 and partly by grants from the BioGreen21 Program, RDA (no. 20070401-034-030) and from KOSEF, Republic of Korea (no. R01-2008-000-10549-0).

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Authors and Affiliations

  1. Department of Anatomy, Embryology Lab, Brain Korea 21 Project for Medical Science, College of Medicine, Yonsei University, 134 Seodaemun-gu, Shinchon-dong, 120-752, Seoul, South Korea

    Yan-Jun Zheng, Hyun Joo Chung, Hyehyun Min, Myengmo Kang, Jogeswar Gadi & Myoung Hee Kim

  2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China

    Yan-Jun Zheng

  3. Brain Korea 21 Project for Medical Science, College of Medicine, Yonsei University, Seoul, 120-752, South Korea

    Hyun Joo Chung, Hyehyun Min & Myoung Hee Kim

  4. Center for Drug Discovery Technologies, Korea Research Institute of Chemical Technology, Daejeon, South Korea

    Seong Hwan Kim

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  1. Yan-Jun Zheng
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  2. Hyun Joo Chung
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  7. Myoung Hee Kim
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Correspondence to Myoung Hee Kim.

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Zheng, YJ., Chung, H.J., Min, H. et al. In Vitro Osteoblast Differentiation is Negatively Regulated by Hoxc8. Appl Biochem Biotechnol 160, 891–900 (2010). https://doi.org/10.1007/s12010-009-8558-3

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  • DOI: https://doi.org/10.1007/s12010-009-8558-3

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