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An α-Glucuronidase Enzyme Activity Assay Adaptable for Solid Phase Screening

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Abstract

Glucuronic acid is a common chemical moiety that decorates the xylan polymer of hemicellulose. This chemical substituent impairs both enzymatic and acidic hydrolysis of xylosidic bonds. The α-glucuronidase enzyme hydrolyzes the 1,2-linked glucuronic acid from the terminal, non-reducing xylose of xylo-oligosaccharides. There are relatively few α-glucuronidase genes in the public databases. We have developed an assay with commercially available reagents that can be used to search DNA libraries for α-glucuronidase genes in a high-throughput, solid phase activity screen.

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Authors and Affiliations

  1. USDA–ARS, 800 Buchanan Street, Albany, CA, 94710, USA

    Charles C. Lee, Kurt Wagschal, Rena E. Kibblewhite-Accinelli, William J. Orts, George H. Robertson & Dominic W. S. Wong

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  1. Charles C. Lee
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  2. Kurt Wagschal
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  3. Rena E. Kibblewhite-Accinelli
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  4. William J. Orts
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  5. George H. Robertson
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  6. Dominic W. S. Wong
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Correspondence to Charles C. Lee.

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Reference to a company and/or products is only for purposes of information and does not imply approval or recommendation of the product to the exclusion of others which may also be suitable. All programs and services of the U.S. Department of Agriculture are offered on a non-discriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap.

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Lee, C.C., Wagschal, K., Kibblewhite-Accinelli, R.E. et al. An α-Glucuronidase Enzyme Activity Assay Adaptable for Solid Phase Screening. Appl Biochem Biotechnol 155, 11–17 (2009). https://doi.org/10.1007/s12010-008-8408-8

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  • DOI: https://doi.org/10.1007/s12010-008-8408-8

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