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Application of Calcium Alginate–Starch Entrapped Bitter Gourd (Momordica charantia) Peroxidase for the Removal of Colored Compounds from a Textile Effluent in Batch as well as in Continuous Reactor

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Abstract

Calcium alginate–starch entrapped bitter gourd peroxidase has been employed for the treatment of a textile industrial effluent in batch as well as in continuous reactor. The textile effluent was recalcitrant to decolorization by bitter gourd peroxidase; thus, its decolorization was examined in the presence of a redox mediator, 1.0 mM 1-hydroxybenzotriazole. Immobilized enzyme exhibited same pH and temperature optima for effluent decolorization as attained by soluble enzyme. Immobilized enzyme could effectively remove more than 70% of effluent color in a stirred batch process after 3 h of incubation. Entrapped bitter gourd peroxidase retained 59% effluent decolorization reusability even after its tenth repeated use. The two-reactor system containing calcium alginate–starch entrapped enzyme retained more than 50% textile effluent decolorization efficiency even after 2 months of its operation. The absorption spectra of the treated effluent exhibited a marked difference in the absorption at various wavelengths as compared to untreated effluent. The use of a two-reactor system containing immobilized enzyme and an adsorbent will be significantly successful for treating industrial effluents at large scale, and it will help in getting water free from aromatic pollutants.

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Abbreviations

BGP:

bitter gourd peroxidase

Con A:

concanavalin A

I-BGP:

immobilized peroxidase

S-BGP:

soluble BGP

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Acknowledgements

University Grants Commission and Department of Science and Technology, New Delhi, Government of India is gratefully acknowledged for providing special grants to the Department in the form of DRS and FIST.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, India

    Mahreen Matto, Rukhsana Satar & Qayyum Husain

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  1. Mahreen Matto
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  2. Rukhsana Satar
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  3. Qayyum Husain
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Correspondence to Qayyum Husain.

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Matto, M., Satar, R. & Husain, Q. Application of Calcium Alginate–Starch Entrapped Bitter Gourd (Momordica charantia) Peroxidase for the Removal of Colored Compounds from a Textile Effluent in Batch as well as in Continuous Reactor. Appl Biochem Biotechnol 158, 512–523 (2009). https://doi.org/10.1007/s12010-008-8396-8

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  • DOI: https://doi.org/10.1007/s12010-008-8396-8

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