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Enhanced Production of Coenzyme Q10 by Overexpressing HMG-CoA Reductase and Induction with Arachidonic Acid in Schizosaccharomyces pombe

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Abstract

Coenzyme Q10 (CoQ10) is a vitamin-like substance which plays a crucial role in the respiratory chain ranging from bacteria to humans and in the radical scavenging in human body. In this study, the full-length hmgR gene (encoding 3-hydroxy-3-methyl-glutaryl-CoA reductase, HMG-CoA reductase) was cloned and overexpressed in Schizosaccharomyces pombe. Using the pREPG yeast depressed under the thiamine as the control, CoQ10 contents increased up to 2.68 and 3.09 times when recombinant cells were incubated without and with arachidonic acid, respectively. It demonstrated that arachidonic acid could upregulate the activity of HMG-CoA reductase and that hmgR gene played a significant role in CoQ10 biosynthesis. So, it has an importance to be utilized for fermentation.

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Acknowledgments

The authors acknowledge financial support from the Natural Science Foundation of China (20576010) and the Program for New Century Excellent Talents (NCET-05-0117). The authors would also express their gratitude to Dr. Xia for providing support in this work and Dr. Carr for providing the expressing vectors.

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Authors and Affiliations

  1. Laboratory of Bioprocessing of Beijing, Beijing University of Chemical Technology, Beijing, People’s Republic of China

    Bing Cheng, Qi-peng Yuan, Xin-xiao Sun & Wen-jin Li

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  1. Bing Cheng
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  2. Qi-peng Yuan
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  3. Xin-xiao Sun
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  4. Wen-jin Li
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Correspondence to Qi-peng Yuan.

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Cheng, B., Yuan, Qp., Sun, Xx. et al. Enhanced Production of Coenzyme Q10 by Overexpressing HMG-CoA Reductase and Induction with Arachidonic Acid in Schizosaccharomyces pombe . Appl Biochem Biotechnol 160, 523–531 (2010). https://doi.org/10.1007/s12010-008-8386-x

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  • DOI: https://doi.org/10.1007/s12010-008-8386-x

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