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Hydrolytic Properties of a Hybrid Xylanase and Its Parents

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Abstract

The hydrolytic properties of a hybrid xylanase (ATx) and its parents (reAnxA and reTfxA) were studied using xylans and xylooligosaccharides as substrates. Analysis of reaction mixtures by high-performance liquid chromatograph revealed that xylotriose (X3) was the main product released from birchwood xylan and wheat bran insoluble xylan by ATx and reAnxA, respectively. Xylobiose (X2) was the main product separately released from birchwood xylan and wheat bran insoluble xylan by reTfxA. Xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) could be hydrolyzed by ATx, which showed no activity on X2 and X3. Therefore, X4 might be the minimum oligomer hydrolyzed by ATx. X2–X6 could be hydrolyzed by reAnxA and reTfxA, respectively. All of ATx, reAnxA, and reTfxA showed transglycosylation activity.

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Abbreviations

ATx:

a hybrid xylanase whose parents are Thermomonospora fusca xylanase A and Aspergillus niger xylanase A

HPLC:

high-performance liquid chromatography

X:

xylose

X2:

xylobiose

X3:

xylotriose

X4:

xylotetraose

X5:

xylopentaose

X6:

xylohexaose

AFM:

atomic force microscope

DP:

degree of polymerization

XOs:

xylooligosaccharides

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Acknowledgment

This work was supported by the National High-Tech Research and Development Plan (2007AA100601), and was also supported by the Science and Technology Department of Zhejinag Province of China (2006C12036).

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Authors and Affiliations

  1. Microbiology Division, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou, 310029, People’s Republic of China

    Jian-Yi Sun & Ming-Qi Liu

  2. College of Life Science, China JiLiang University, Hangzhou, 310018, China

    Ming-Qi Liu

  3. College of Life Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Xiao-Yan Weng

  4. Department of Biological Science, College of Life Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Xiao-Yan Weng

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  1. Jian-Yi Sun
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Correspondence to Xiao-Yan Weng.

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Sun, JY., Liu, MQ. & Weng, XY. Hydrolytic Properties of a Hybrid Xylanase and Its Parents. Appl Biochem Biotechnol 152, 428–439 (2009). https://doi.org/10.1007/s12010-008-8316-y

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