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Preparation and Application of Monoclonal Antibody Against hNDRG2

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Abstract

The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi’an, Shaanxi, 710032, People’s Republic of China

    Xinping Liu, Xiaolan Hu, Jian Zhang, Lifeng Wang, Wenghong Zhang, Fuyang Li & Libo Yao

  2. Department of Immunology, The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi’an, Shaanxi, 710032, People’s Republic of China

    Xuesong Liu

  3. Department of Histology and Embryology, The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi’an, Shaanxi, 710032, People’s Republic of China

    Yuanqiang Zhang

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  1. Xinping Liu
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  2. Xiaolan Hu
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  3. Jian Zhang
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  4. Lifeng Wang
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  5. Wenghong Zhang
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  6. Xuesong Liu
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  7. Fuyang Li
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  8. Yuanqiang Zhang
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  9. Libo Yao
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Correspondence to Libo Yao.

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Liu, X., Hu, X., Zhang, J. et al. Preparation and Application of Monoclonal Antibody Against hNDRG2. Appl Biochem Biotechnol 152, 306–315 (2009). https://doi.org/10.1007/s12010-008-8267-3

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  • DOI: https://doi.org/10.1007/s12010-008-8267-3

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