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Survival of Freeze-dried Leuconostoc mesenteroides and Lactobacillus plantarum Related to Their Cellular Fatty Acids Composition during Storage

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Abstract

Lactic acid bacteria strains Lactobacillus plantarum CWBI-B534 and Leuconostoc ssp. mesenteroïdes (L. mesenteroïdes) Kenya MRog2 were produced in bioreactor, concentrated, with or without cryoprotectants. In general, viable population did not change significantly after freeze-drying (p > 0.05). In most cases, viable population for cells added with cryoprotectants was significantly lower than those without (p < 0.05). Cellular fatty acids (CFAs) from the two strains in this study were analyzed before and after freeze-drying. Six CFAs were identified, namely, palmitic (C16:0), palmitoleic (C16:1), stearic (C18:0), oleic (C18:1), linoleic (C18:2), and linolenic (C18:3) acids were identified. Four of them, C16:0, C16:1, C18:0, and C18:1, make up more than 94% or 93% of the fatty acids in L. mesenteroides and L. plantarum, respectively, with another one, namely, C18:3, making a smaller (on average 5–6%, respectively) contribution. The C18:2 contributed very small percentages (on average ≤ 1%) to the total in each strain. C16:0 had the highest proportion at most points relative to other fatty acids. Moisture content and water activity (a w) increased significantly during the storage period. It was observed that C16:1/C16:0, C18:0/C16:0 and C18:1/C16:0 ratios for freeze-dried L. mesenteroides or L. plantarum, with or without cryoprotectants, did not change significantly during the storage period. According to the packaging mode and storage temperatures, C18:2/C16:0 and C18:3/C16:0 ratios for freeze-dried L. mesenteroides and L. plantarum with or without cryoprotectants decreased as the storage time increased. However, a higher C18:2/C16:0 or C18:3/C16:0 ratio for L. mesenteroides and L. plantarum was noted in the freeze-dried powder held at 4 °C or under vacuum and in dark than at 20 °C or in the presence of oxygen and light.

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Abbreviations

a w :

water activity

CFA:

cellular fatty acid

K :

Inactivation rate

k a :

acidification loss rate

L :

Leuconostoc

LAB:

Lactic acid bacteria

Lb :

Lactobacillus

Me:

Methyl esterified

MUFA:

Monounsaturated fatty acids

P:

cell without cryoprotectants

PC:

cell with cryoprotectants

PUFAs:

Polyunsaturated fatty acids

R 2 :

Determination coefficient

RH:

Relative humidity

S:

saturated fatty acids

Tg:

Temperature of glass transition

Ts:

Surface temperature of product

U:

Unsaturated fatty acids

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Acknowledgments

The authors would like to acknowledge the Federal Research Centre for Nutrition, Institute of Hygiene and Toxicology, Karlsruhe, Germany for providing L. mesenteroides ssp. mesenteroides Kenya MRog2 strain and Mrs Maryse Hardenne and Mr Philippe Mottet for their contribution. We thank all the technical personal of CWBI (Centre Wallon de Biologie Industrielle). We also express our gratitude to the republic of Ivory Cost and the communauté française de Belgique for financial assistance.

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Authors and Affiliations

  1. Unité des Bio-industries, Centre Wallon de Biologie Industrielle (CWBI), Faculté Universitaire des Sciences Agronomiques de Gembloux (FUSAGx), Passage des Déportés 2, B-5030, Gembloux, Belgium

    Ibourahema Coulibaly & Philippe Thonart

  2. Service de Technologie Microbienne, Centre Wallon de Biologie Industrielle (CWBI), Université de Liège (ULg), Sart Tilman, B40, B-4000, Liège, Belgium

    Anastasie Yao Amenan & Philippe Thonart

  3. Unité de Biologie végétale (BV), Faculté Universitaire des Sciences Agronomiques de Gembloux (FUSAGx), Passage des Déportés 2, B-5030, Gembloux, Belgium

    Marie Laure Fauconnier

  4. Unité de Chimie Analytique (CA), Faculté Universitaire des Sciences Agronomiques de Gembloux (FUSAGx), Passage des Déportés 2, B-5030, Gembloux, Belgium

    Georges Lognay

Authors
  1. Ibourahema Coulibaly
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  2. Anastasie Yao Amenan
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  3. Georges Lognay
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  4. Marie Laure Fauconnier
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  5. Philippe Thonart
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Corresponding authors

Correspondence to Ibourahema Coulibaly or Anastasie Yao Amenan.

Additional information

Coulibaly Ibourahema and Yao Amenan Anastasie equally contributed to this work.

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Coulibaly, I., Amenan, A.Y., Lognay, G. et al. Survival of Freeze-dried Leuconostoc mesenteroides and Lactobacillus plantarum Related to Their Cellular Fatty Acids Composition during Storage. Appl Biochem Biotechnol 157, 70–84 (2009). https://doi.org/10.1007/s12010-008-8240-1

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  • DOI: https://doi.org/10.1007/s12010-008-8240-1

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