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Optimization of Culture Conditions for Enhanced Decolorization of Cibacron Red FN-2BL by Schizophyllum commune IBL-6

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Abstract

The objective of this study was to exploit the decolorization potential of a newly isolated white-rot fungus Schizophyllum commune IBL-6 for the biodegradation of reactive textile dye Cibacron Red FN-2BL. In the initial decolorization study of 10 days, it was observed that S. commune IBL-6 was a better decolorizer of Cibacron Red FN-2BL. Various process parameters like composition of basal nutrient medium, pH, temperature, additional carbon and nitrogen sources, and initial dyestuff concentration were optimized to develop an economic decolorization process. The optimum dye decolorization was achieved in basal nutrient medium II containing 0.1% Cibacron Red FN-2BL and supplemented with 1% glucose after 3 days incubation at pH 4.5 and 30 °C. All the additional carbon sources were found to enhance decolorization process, whereas most of the nitrogen supplements caused fungal-growth inhibition. The pattern of enzymes involved in the biodegradation of this dye was studied, and manganese peroxidase was found to be the major peroxidase with minor lignin peroxidase and laccase activities.

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Acknowledgment

The manuscript is a part of the project funded by Higher Education Commission (HEC) of Pakistan. The funding by HEC is thankfully acknowledged.

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Authors and Affiliations

  1. Industrial Biotechnology Laboratory, Department of Chemistry, University of Agriculture, Faisalabad, Faisalabad, 38040, Pakistan

    Haq Nawaz Bhatti, Nadia Akram & Muhammad Asgher

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  1. Haq Nawaz Bhatti
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  2. Nadia Akram
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  3. Muhammad Asgher
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Correspondence to Haq Nawaz Bhatti.

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Bhatti, H.N., Akram, N. & Asgher, M. Optimization of Culture Conditions for Enhanced Decolorization of Cibacron Red FN-2BL by Schizophyllum commune IBL-6. Appl Biochem Biotechnol 149, 255–264 (2008). https://doi.org/10.1007/s12010-007-8123-x

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  • DOI: https://doi.org/10.1007/s12010-007-8123-x

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