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Enhanced l -(+)-Lactic Acid Production by an Adapted Strain of Rhizopus oryzae using Corncob Hydrolysate

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Abstract

Corncob is an economic feedstock and more than 20 million tons of corncobs are produced annually in China. Abundant xylose can be potentially converted from the large amount of hemicellulosic materials in corncobs, which makes the crop residue an attractive alternative substrate for a value-added production of a variety of bioproducts. Lactic acid can be used as a precursor for poly-lactic acid production. Although current industrial lactic acid is produced by lactic acid bacteria using enriched medium, production by Rhizopus oryzae is preferred due to its exclusive formation of the l-isomer and a simple nutrition requirement by the fungus. Production of l-(+)-lactic acid by R. oryzae using xylose has been reported; however, its yield and conversion rate are poor compared with that of using glucose. In this study, we report an adapted R. oryzae strain HZS6 that significantly improved efficiency of substrate utilization and enhanced production of l-(+)-lactic acid from corncob hydrolysate. It increased l-(+)-lactic acid final concentration, yield, and volumetric productivity more than twofold compared with its parental strain. The optimized growth and fermentation conditions for Strain HZS6 were defined.

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Acknowledgment

The authors are grateful to Prof. S. L. Yang (Chinese Academy of Engineering) for the helpful suggestions. This study was supported in part by a grant of UK DTI-China MOST Collaborative Research and USDA National Program 307/306.

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Authors and Affiliations

  1. Center for Microbial Biotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark

    Dong-Mei Bai

  2. Institute of New Energy Technology, Tsinghua University, Beijing, 100084, People’s Republic of China

    Shi-Zhong Li

  3. U.S. Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, IL, 61604, USA

    Z. Lewis Liu

  4. Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UK

    Zhan-Feng Cui

Authors
  1. Dong-Mei Bai
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  2. Shi-Zhong Li
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  3. Z. Lewis Liu
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  4. Zhan-Feng Cui
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Corresponding authors

Correspondence to Shi-Zhong Li or Z. Lewis Liu.

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Bai, DM., Li, SZ., Liu, Z.L. et al. Enhanced l -(+)-Lactic Acid Production by an Adapted Strain of Rhizopus oryzae using Corncob Hydrolysate. Appl Biochem Biotechnol 144, 79–85 (2008). https://doi.org/10.1007/s12010-007-8078-y

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  • DOI: https://doi.org/10.1007/s12010-007-8078-y

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