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Antibacterial noncytotoxic chitosan coatings on polytetrafluoroethylene films by plasma grafting for medical device applications

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Abstract

Chitosan is an exciting alternative for the development of coating-surfaces due to its large action spectrum against pathogenic microorganisms. However, to produce a stable coating with effective antibacterial action, a compromise between deacetylation degree (DD) and molecular weight (MW) is essential. Four chitosan samples were characterized regarding Mw and DD and correlated with the minimum and bactericide concentrations against E. coli, P. aeruginosa, and S. aureus. CHI80MW (79.7% DD and 7.0 × 105 Da) showed the best antibacterial effect and was selected to functionalize polytetrafluoroethylene (PTFE) surfaces by plasma. CHI80MW was grafted onto the PTFE surfaces using two different spacer molecules: poly(ethylene glycol) bis (carboxymethyl) ether (PEG) and poly(ethylene-alt-maleic anhydride) (PA). PTFE-Plasma-PA-CHI80MW exhibited a coating with more attached chitosan and better antibacterial action if compared to PTFE-Plasma-PEG-CHI80MW: after 8 h, PTFE-Plasma-PEG-CHI80MW presented a bacterial reduction of 25-30% for the three bacterial strains, and PTFE-Plasma-PA-CHI80MW reduced them to 77-90%. Moreover, cytotoxicity tests showed that PTFE-Plasma-PA-CHI80MW samples were compatible with human fibroblasts.

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Data availability

All the data included in this study are available upon request by contacting the corresponding author.

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Funding

This work was supported by the Sao Paulo Research Foundation (Grant No 2013/05135-1), Coordination for the Improvement of Higher Education Personnel (CAPES: Procad 88882.151600/2017-01, Proex 33003017034P8), and National Council for Scientific and Technological Development (CNPq 421039/2016-7).

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of Ceara, Campus do Pici - Bloco 709, Fortaleza, Ceara, 60455-760, Brazil

    Juliana M. Vaz, Larissa M. C. G. Fiúza & Rodrigo S. Vieira

  2. Department of Materials Engineering and Bioprocess, School of Chemical Engineering, University of Campinas, Building D, Campinas, Sao Paulo, 13083-852, Brazil

    Thiago B. Taketa & Marisa M. Beppu

  3. Department of Physical and Mathematical Sciences, Catholic University of Temuco, Building CT, 4813302, Temuco, La Araucanía, Chile

    Jacobo Hernandez-Montelongo

  4. Department of Basic Health Sciences, Laboratory of Cell Biology, Federal University of Health Sciences of Porto Alegre - UFCSPA, Porto Alegre, RS, 90050-170, Brazil

    Cristiano Rodrigues

Authors
  1. Juliana M. Vaz
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  2. Thiago B. Taketa
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  3. Jacobo Hernandez-Montelongo
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  4. Larissa M. C. G. Fiúza
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  5. Cristiano Rodrigues
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  6. Marisa M. Beppu
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  7. Rodrigo S. Vieira
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Contributions

JMV and RSV conceived and designed experiments; LMCGF performed the characterization of chitosan powders and obtained the MIC and MBC values for the chitosan powders; JMV functionalized the PTFE surfaces with chitosan by plasma grafting; JHM performed the photoluminescence characterization; JMV performed the antibacterial tests; CR performed the cytocompatibility tests; JMV, CR, and JHM analyzed the data; MMB and RSV contributed reagents/materials/analysis tools; JMV, TBT, JHM, and RSV wrote the manuscript. All authors revised the manuscript.

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Correspondence to Jacobo Hernandez-Montelongo or Rodrigo S. Vieira.

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Vaz, J.M., Taketa, T.B., Hernandez-Montelongo, J. et al. Antibacterial noncytotoxic chitosan coatings on polytetrafluoroethylene films by plasma grafting for medical device applications. J Coat Technol Res 19, 829–838 (2022). https://doi.org/10.1007/s11998-021-00560-3

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  • DOI: https://doi.org/10.1007/s11998-021-00560-3

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