Abstract
Eggs are an important, low-cost, high-protein food with a balanced nutritional composition. The sanitary quality of commercial eggs is a bottleneck that needs to be overcome through the use of technologies that guarantee the safety of the product. This work aimed to prepare, characterize, and evaluate the coating of commercial eggs by chitosan filmogenic solutions and 1st- and 5th-generation quaternary ammonium salt components (QACs). Egg shell properties such as thickness, morphology, and wettability were analyzed by scanning electron microscopy (SEM),TG/DSC, pHPZC, contact angle, color, and weight loss. Determinations of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) with Salmonella spp., Escherichia coli, and Staphylococcus aureus were performed, as well as proliferation tests on eggshell surfaces. The results show a synergistic effect of the biopolymer in terms of antibacterial activity against all microorganisms tested. The composite of chitosan and ammonium quaternary ammonium films offers physical and biological safety to guarantee the integrity and microbiological quality of commercial eggs. Egg coating using bioactive chitosan films was shown to be a suitable method of preservation and a physical and microbiological barrier to minimize egg quality loss during storage, as well as to increase shelf life.
Graphical abstract
The use of chitosan and quaternary ammonium salt films offers a physical and biological safety to guarantee the integrity and microbiological quality of eggs.
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Abbreviations
- QAC 1st:
-
1st generation of quaternary ammonium salt compounds
- QAC 5th:
-
5th generation of quaternary ammonium salt compounds
- FAPESP:
-
Fundação de Amparo à Pesquisa do Estado de São Paulo
- CEPID:
-
Centro de Pesquisa, Inovação e Difusão
- INCTMN:
-
Instituto Nacional de Ciência e Tecnologia dos Materiais em Nanotecnologia
- CNPq:
-
Conselho Nacional de Desenvolvimento Científico e Tecnológico
- MIC:
-
Minimal inhibitory concentration
- MBC:
-
Minimal bactericidal concentration
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Acknowledgments
The authors give special thanks to PolyOrganic Technologic Ltda, São Paulo, SP, Polymar Indústria e Comércio Ltda, Fortaleza, CE, Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT), and Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES). Special thanks to Márcio Roberto da Silva Oliveira for their contributions in contact angle analyses.
Funding
This work was supported by FAPESP (grant numbers 2012/07067-0,2013/23572-0; 2016/019405; and 2013/07296); CEPID (2013/07296-2); INCTMN (2008/57872-1); and CNPq (573636/2008-7, 435975/2018-8, and 421648/2018-0).
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Synopsis
Towards a green biopackaging material for eggs, natural polymeric antimicrobial coatings were developed and investigated to improve sanitary quality and extend shelf life.
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Almeida e Silva, T., Gorup, L.F., de Araújo, R.P. et al. Synergy of Biodegradable Polymer Coatings with Quaternary Ammonium Salts Mediating Barrier Function Against Bacterial Contamination and Dehydration of Eggs. Food Bioprocess Technol 13, 2065–2081 (2020). https://doi.org/10.1007/s11947-020-02545-3
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DOI: https://doi.org/10.1007/s11947-020-02545-3