Abstract
Edible films and coatings can be used for packaging food, thus reducing the consumption of plastics in daily food products, and can also be enriched with active compounds to improve food functionality. The main goal of the current research was to produce edible films incorporating elderberry extract, an active ingredient rich in polyphenols that exhibits several therapeutic characteristics, to allow its protection and controlled release. To protect the active compound, different biopolymers were used (modified chitosan, sodium alginate and gum arabic) and films with different thicknesses were produced, by heat-induced gelation, using the conventional solvent casting method. Then, the formulations were evaluated to compare their properties in terms of entrapment efficiency, morphology, optical properties and release behaviour. The films exhibited entrapment efficiencies for polyphenols between 74.0 and 99.9%, presenting homogenous structures, which were predominantly transparent, flexible and yellowish, with a morphology that varied with the entrapment agent. The release of the polyphenols also depended on the entrapment agent used to protect them, with a faster release occurring for the films with gum arabic, followed by modified chitosan and, finally, by sodium alginate. The films’ thickness did not significantly affect the colour, morphology and release of the active agent. Using mathematical modelling, it was verified that, in general, the zero-order, the Korsmeyer-Peppas and the Weibull models are the kinetic equations producing a better adjustment to the experimental release profiles, with correlation coefficients that ranged from 0.981 to 0.996, 0.987 to 0.997 and 0.984 to 0.999, respectively. It can be concluded that films would be a good alternative to other systems for the delivery of active compounds, allowing the replacement of plastic materials for protecting food products.
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References
Aliakbarian, B., Paini, M., & Alberto, A. (2015). Effect of encapsulating agent on physical-chemical characteristics of olive pomace polyphenols-rich extracts. Chemical Engineering Transactions, 43, 97–102. https://doi.org/10.3303/CET1543017.
Aparicio-Fernández, X., Vega-Ahuatzin, A., Ochoa-Velasco, C. E., Cid-Pérez, S., Hernández-Carranza, P., & Ávilla-Sosa, R. (2018). Physical and antioxidant characterization of edible films added with red prickly pear (Opuntia ficus-indica L .) cv . San Martín peel and/or its aqueous extracts. Food and Bioprocess Technology, 11(2), 368–379. https://doi.org/10.1007/s11947-017-2017-x.
Calva-Estrada, S. J., Jiménez-Fernández, M., & Lugo-Cervantes, E. (2019). Protein-based films: advances in the development of biomaterials applicable to food packaging. Food Engineering Reviews, 11(2), 78–92. https://doi.org/10.1007/s12393-019-09189-w.
Cardoso, T., Gonçalves, A., Estevinho, B. N., & Rocha, F. (2019). Potential food application of resveratrol microparticles: characterization and controlled release studies. Powder Technology, 355, 593–601. https://doi.org/10.1016/j.powtec.2019.07.079.
Chiralt, A., Vargas, M., & Atarés, L. (2018). Edible films and coatings from proteins. In Proteins in Food Processing (Second ed.). Elsevier Ltd. https://doi.org/10.1016/B978-0-08-100722-8.00019-X.
Costa, R., & Santos, L. (2017). Delivery systems for cosmetics - from manufacturing to the skin of natural antioxidants. Powder Technology, 322, 402–416. https://doi.org/10.1016/j.powtec.2017.07.086.
Costa, M. J., Maciel, L. C., Teixeira, J. A., Vicente, A. A., & Cerqueira, M. A. (2018). Use of edible films and coatings in cheese preservation: opportunities and challenges. Food Research International, 107(February), 84–92. https://doi.org/10.1016/j.foodres.2018.02.013
Darbasi, M., Askari, G., Kiani, H., & Khodaiyan, F. (2017). Development of chitosan based extended-release antioxidant films by control of fabrication variables. International Journal of Biological Macromolecules, 104(Pt A), 303–310. https://doi.org/10.1016/j.ijbiomac.2017.06.055.
Dawidowicz, A. L., Wianowska, D., & Baraniak, B. (2006). The antioxidant properties of alcoholic extracts from Sambucus nigra L. (antioxidant properties of extracts). LWT - Food Science and Technology, 39(3), 308–315. https://doi.org/10.1016/j.lwt.2005.01.005.
De Dicastillo, C. L., Rodríguez, F., Guarda, A., & Galotto, M. J. (2016). Antioxidant films based on cross-linked methyl cellulose and native Chilean berry for food packaging applications. Carbohydrate Polymers, 136, 1052–1060. https://doi.org/10.1016/j.carbpol.2015.10.013.
Dehghani, S., Vali, S., & Regenstein, J. M. (2018). Edible films and coatings in seafood preservation: a review. Food Chemistry, 240, 505–513. https://doi.org/10.1016/j.foodchem.2017.07.034.
Dong, Y., Martin, K., He, S., & Balling, S. (2017). Gum Arabic authentication and mixture quantification by near infrared spectroscopy. Food Control, 78, 144–149. https://doi.org/10.1016/j.foodcont.2017.02.002.
Estevinho, B. N., & Rocha, F. (2017a). Kinetic models applied to soluble vitamins delivery systems prepared by spray drying. Drying Technology, 35(10), 0-9. https://doi.org/10.1080/07373937.2016.1242015
Estevinho, B. N., & Rocha, F. (2017b). A key for the future of the flavors in food industry. In Nanotechnology applications in food (pp. 1–19). Elsevier. https://doi.org/10.1016/B978-0-12-811942-6.00001-7.
Estevinho, B. N., Rocha, F., Santos, L., & Alves, A. (2013a). Using water soluble chitosan for flavour microencapsulation in food industry. Journal of Microencapsulation, 30(6), 571–579. https://doi.org/10.3109/02652048.2013.764939.
Estevinho, B. N., Rocha, F., Santos, L., & Alves, A. (2013b). Microencapsulation with chitosan by spray drying for industry applications – a review. Trends in Food Science and Technology, 31(2), 138–155. https://doi.org/10.1016/j.tifs.2013.04.001.
Fu, Y., Sarkar, P., Bhunia, A. K., & Yao, Y. (2016). Delivery systems of antimicrobial compounds to food. Trends in Food Science and Technology, 57, 165–177. https://doi.org/10.1016/j.tifs.2016.09.013.
Ganiari, S., Choulitoudi, E., & Oreopoulou, V. (2017). Edible and active films and coatings as carriers of natural antioxidants for lipid food. Trends in Food Science and Technology, 68, 70–82. https://doi.org/10.1016/j.tifs.2017.08.009.
Horciu, L., Estevinho, B. N., Kloetzer, L., & Blaga, A. (2016). Ultrasound-Assisted extraction of polyphenols from clove (Syzygium aromaticum).
Karimi, Y., Maftoonazad, N., Ramanswamy, H. S., Prasher, S. O., & Marcotte, M. (2012). Application of hyperspectral technique for color classification avocados subjected to different treatments. Food and Bioprocess Technology, 5(1), 252–264. https://doi.org/10.1007/s11947-009-0292-x.
Marquez, G. R., Di Pierro, P., Esposito, M., Mariniello, L., & Porta, R. (2014). Application of transglutaminase-crosslinked whey protein/ pectin films as water barrier coatings in fried and baked foods. Food and Bioprocess Technology, 7(2), 447–455. https://doi.org/10.1007/s11947-012-1045-9.
Norajit, K., Myong, K., & Hyung, G. (2010). Comparative studies on the characterization and antioxidant properties of biodegradable alginate films containing ginseng extract. Journal of Food Engineering, 98(3), 377–384. https://doi.org/10.1016/j.jfoodeng.2010.01.015.
Ozdemir, M., & Floros, J. D. (2001). Analysis and modeling of potassium sorbate diffusion through edible whey protein films. Journal of Food Engineering, 47(2), 149–155. https://doi.org/10.1016/S0260-8774(00)00113-8.
Poverenov, E., Rutenberg, R., Danino, S., Horev, B., & Rodov, V. (2014). Gelatin-chitosan composite films and edible coatings to enhance the quality of food products: layer-by-layer vs . blended formulations. Food and Bioprocess Technology, 7(11), 3319–3327. https://doi.org/10.1007/s11947-014-1333-7.
Reyes-Avalos, M. C., Minjares-Fuentes, R., Femenia, A., Contreras-Esquivel, J. C., Quintero-Ramos, A., Esparza-Rivera, J. R., & Meza-Velásquez, J. A. (2019). Application of an alginate – chitosan edible film on figs (Ficus carica): effect on bioactive compounds and antioxidant capacity. Food and Bioprocess Technology, 12(3), 499–511. https://doi.org/10.1007/s11947-018-2226-y.
Ribeiro, A. M., Estevinho, B. N., & Rocha, F. (2019). Spray drying encapsulation of elderberry extract and evaluating the release and stability of phenolic compounds in encapsulated powders. Food and Bioprocess Technology, 12(8), 1381–1394. https://doi.org/10.1007/s11947-019-02304-z.
Robles-Flores, G. d. C., Abud-Archila, M., Ventura-Canseco, L. M. C., Meza-Gordillo, R., Grajales-Lagunes, A., Ruiz-Cabrera, M. A., & Gutiérrez-Miceli, F. A. (2018). Development and evaluation of a film and edible coating obtained from the Cajanus cajan seed applied to fresh strawberry fruit. Food and Bioprocess Technology, 11(12), 2172–2181. https://doi.org/10.1007/s11947-018-2175-5.
Rubilar, R. F., Cruz, R. M. S., Zuñiga, R. N., Khmelinskii, I., & Vieira, M. C. (2017). Mathematical modeling of gallic acid release from chitosan films with grape seed extract and carvacrol. International Journal of Biological Macromolecules, 104(Pt A), 197–203. https://doi.org/10.1016/j.ijbiomac.2017.05.187.
Setti, C., Suarato, G., Perotto, G., Athanassiou, A., & Bayer, I. S. (2018). Investigation of in vitro hydrophilic and hydrophobic dual drug release from polymeric films produced by sodium alginate-MaterBi® drying emulsions. European Journal of Pharmaceutics and Biopharmaceutics, 130, 71–82. https://doi.org/10.1016/j.ejpb.2018.06.019.
Silva-Weiss, A., Quilaqueo, M., Venegas, O., Ahumada, M., Silva, W., & Osorio, F. (2018). Design of dipalmitoyl lecithin liposomes loaded with quercetin and rutin and their release kinetics from carboxymethyl cellulose edible films. Journal of Food Engineering, 224, 165–173. https://doi.org/10.1016/j.jfoodeng.2018.01.001.
Skog, K. (2018). EU has to step up in fight against plastic waste. https://www.regeringen.se/debattartiklar/2018/03/eu-has-to-step-up-in-fight-against-plastic-waste
Souza, V., Fernando, A., Pires, J., Rodrigues, P., Lopes, A., & Fernandes, F. (2017). Physical properties of chitosan films incorporated with natural antioxidants. Industrial Crops & Products, 107(February), 565–572. https://doi.org/10.1016/j.indcrop.2017.04.056
Talón, E., Trifkovic, K. T., Nedovic, V. A., Bugarski, B. M., Vargas, M., Chiralt, A., & González-Martínez, C. (2017a). Antioxidant edible films based on chitosan and starch containing polyphenols from thyme extracts. Carbohydrate Polymers, 157, 1153–1161. https://doi.org/10.1016/j.carbpol.2016.10.080.
Talón, E., Trifkovic, K. T., Vargas, M., Chiralt, A., & González-Martínez, C. (2017b). Release of polyphenols from starch-chitosan based films containing thyme extract. Carbohydrate Polymers, 175, 122–130. https://doi.org/10.1016/j.carbpol.2017.07.067.
Tosati, J. V., Messias, V. C., Carvalho, P. I. N., Pollonio, M. A. R., Meireles, M. A. A., & Monteiro, A. R. (2017). Antimicrobial effect of edible coating blend based on turmeric starch residue and gelatin applied onto fresh frankfurter sausage. Food and Bioprocess Technology, 10(12), 2165–2175. https://doi.org/10.1007/s11947-017-1985-1.
Ulbin-Figlewicz, N., Zimoch-Korzycka, A., & Jarmoluk, A. (2014). Antibacterial activity and physical properties of edible chitosan films exposed to low-pressure plasma. Food & Function, 7(12), 3646–3654. https://doi.org/10.1007/s11947-014-1379-6.
Xu, T., Gao, C., Yang, Y., Shen, X., Huang, M., & Liu, S. (2018). Retention and release properties of cinnamon essential oil in antimicrobial films based on chitosan and gum arabic. Food Hydrocolloids, 84, 84–92. https://doi.org/10.1016/j.foodhyd.2018.06.003.
Acknowledgements
Berta N. Estevinho would like to thank the Fundação para a Ciência e a Tecnologia (FCT) for the contract based on the “Lei do Emprego Científico” (DL 57/2016).
Funding
This work was financially supported by the following: Base Funding - UIDB/00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE - funded by national funds through the FCT/MCTES (PIDDAC); Project POCI-01-0145-FEDER-028715 (MicroDelivery - Development of controlled delivery functional systems by microencapsulation of natural and active compounds with therapeutic, nutritional and technological interest), funded by FEDER funds through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) and by national funds (PIDDAC) through FCT/MCTES.
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Ribeiro, A.M., Estevinho, B.N. & Rocha, F. Edible Films Prepared with Different Biopolymers, Containing Polyphenols Extracted from Elderberry (Sambucus Nigra L.), to Protect Food Products and to Improve Food Functionality. Food Bioprocess Technol 13, 1742–1754 (2020). https://doi.org/10.1007/s11947-020-02516-8
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DOI: https://doi.org/10.1007/s11947-020-02516-8