Skip to main content
Log in

Application of Pulsed Light to Sliced Cheese: Effect on Listeria Inactivation, Sensory Quality and Volatile Profile

  • Original Paper
  • Published:
Food and Bioprocess Technology Aims and scope Submit manuscript

Abstract

Two cheese varieties with different surface features (Gouda and Manchego) were tested for pulsed light decontamination of Listeria sp. on ready-to-eat presentations. Sensory quality and volatile profile were also studied. Cheese slices were flashed with fluences comprised between 0.9 and 8.4 J/cm2. The treatment was less effective in Manchego than in Gouda, in which 3 log cfu/cm2 were obtained with 0.9 J/cm2. No sensory changes were observed due to the application of this dose. In Manchego slices, the maximum inactivation obtained was lower than 1 log cfu/cm2 even at the highest fluence used. In both cheeses, differences in odour and flavour were reported immediately after treatment for fluences ≥4.2 J/cm2, due to the development of sulfur notes. Volatile analysis showed an increase of sulfur compounds which could explain these observations. The concentration of sulfur volatiles and the corresponding sensory notes disappeared during cold storage. In view of these results, pulsed light could be considered a useful strategy to decontaminate smooth-surface ready-to-eat cheeses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Angelidis, A. S., & Koutsoumanis, K. (2006). Prevalence and concentration of Listeria monocytogenes in sliced ready-to-eat meat products in the Hellenic retail market. Journal of Food Protection, 69, 938–942.

    Google Scholar 

  • Ballesteros, C., Poveda, J. M., González-Viñas, M. A., & Cabezas, L. (2006). Microbiological, biochemical and sensory characteristics of artisanal and industrial Manchego cheeses. Food Control, 17, 249–255.

    Article  CAS  Google Scholar 

  • Can, F. O., Demirci, A., Puri, W. M., & Gourama, H. (2014). Decontamination of hard cheeses by pulsed UV light. Journal of Food Protection, 77, 1723–1731.

    Article  Google Scholar 

  • Choe, E., Huang, R., & Min, D. B. (2005). Chemical reactions and stability of riboflavin in foods. Journal of Food Science, 70, 28–36.

    Article  Google Scholar 

  • Choisy, C., Desmazeaud, J., Gripon C., Lamberet, G., Lenoir J., & Tourneur, C. (1990). Los fenómenos microbiológicos y enzimáticos y la bioquímica del afinado. In A. Eck (Ed.), El queso (pp. 57–91). Barcelona: Omega.

  • Christensen, Z. T., Ogden, L. V., Dunn, M. L., & Eggett, D. L. (2006). Multiple comparison procedures for analysis of ranked data. Journal of Food Science, 71, 132–143.

    Article  Google Scholar 

  • Dalsgaard, T. K., Sorensen, J., Bakman, M., Voghsen, L., Nebesl, C., Albrechtsen, R., & Nielsen, J. (2010). Light-induced protein and lipid oxidation in cheese: dependence on fat content and packaging conditions. Dairy Science and Technology, 90, 565–577.

    Article  CAS  Google Scholar 

  • De Man, J. M. (2000). Chemical and physical properties of fatty acids. In C. K. Chow (Ed.), Fatty acids in foods and their health implications (pp. 17–46). New York: Marcel Dekker.

    Google Scholar 

  • DTU (2009). Danish food composition databank (ed. 7.01). National Food Institute - Technical University of Denmark (DTU). http://www.foodcomp.dk/v7/fcdb_foodcomplist.asp?CompId=0037. Accessed 1 June 2005.

  • Dunn, J. E., Clark, R. W., Asmus, J. F., Pearlman, J. S., Boyer, K., Painchaud, F., et al. (1989). Methods for preservation of foodstuffs. US Patent 4871559.

  • Dunn, J. E., Clark, R. W., Asmus, J. F., Pearlman, J. S., Boyer, K., Painchaud, F., et al. (1991). Methods for preservation of foodstuffs. U.S. Patent 5034235.

  • EFSA. (2013). Analysis of the baseline survey on the prevalence of Listeria monocytogenes in certain ready-to-eat (RTE) foods in the EU, 2010-2011 part A: Listeria monocytogenes prevalence estimates. EFSA Journal, 11(3241), 75.

    Google Scholar 

  • European Commission. (2007). Regulation (EC) No 1441/2007 of 5 December 2007 amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs. Official Journal of the European Union L, 322, 12–29.

    Google Scholar 

  • Fernández, M., Manzano, S., de la Hoz, L., Ordóñez, J. A., & Hierro, E. (2009). Pulsed light inactivation of Listeria monocytogenes through different plastic films. Foodborne Pathogens and Disease, 6, 1265–1267.

    Article  Google Scholar 

  • Fernández, M., Ganan, M., Guerra, C., & Hierro, E. (2014). Protein oxidation in processed cheese slices treated with pulsed light technology. Food Chemistry, 159, 388–390.

    Article  Google Scholar 

  • Frye, C., & Donnelly, C. W. (2005). Comprehensive survey of pasteurized fluid milk produced in the United States reveals a low prevalence of Listeria monocytogenes. Journal of Food Protection, 68, 973–979.

    Google Scholar 

  • Ganan, M., Hierro, E., Hospital, X. F., Barroso, E., & Fernández, M. (2013). Use of pulsed light to increase the safety of ready-to-eat cured meat products. Food Control, 32, 512–517.

    Article  Google Scholar 

  • Gómez-López, V. M., Devlieghere, F., Bonduelle, V., & Debevere, J. (2005). Factors affecting the inactivation of microorganisms by intense light pulses. Journal of Applied Microbiology, 99, 460–470.

    Article  Google Scholar 

  • Gómez-Ruiz, J. A., Cabezas, L., Martínez-Castro, I., González-Viñas, M. A., & Poveda, J. M. (2008). Influence of a defined-strain starter and Lactobacillus plantarum as adjunct culture on volatile compounds and sensory characteristics of Manchego cheese. European Food Research and Technology, 227, 181–190.

    Article  Google Scholar 

  • Guerra, M. M., McLauchlin, J., & Bernardo, F. A. (2001). Listeria in ready-to-eat and unprocessed foods produced in Portugal. Food Microbiology, 18, 423–429.

    Article  Google Scholar 

  • Hierro, E., Barroso, E., Hoz, L., Ordóñez, J. A., Manzano, S., & Fernández, M. (2011). Efficacy of pulsed light for shelf-life extension and inactivation of Listeria monocytogenes on ready-to-eat cooked meat products. Innovative Food Science and Emerging Technologies, 12, 275–281.

    Article  Google Scholar 

  • Hierro, E., Ganan, M., Barroso, B., & Fernández, M. (2012). Pulsed light treatment for the inactivation of selected pathogens and the shelf-life extension of beef and tuna carpaccio. International Journal of Food Microbiology, 158, 42–48.

    Article  Google Scholar 

  • ISO. (1981). Analyse sensorielle guide pour l´implantation d´un local destiné aux analyses sensorielles. (ISO-DP 6658). Genéve, Switzerland: International Organization for Standardization.

    Google Scholar 

  • Joanes, D. N. (1985). On a rank sum test due to Kramer. Journal of Food Science, 50, 1442–1444.

    Article  Google Scholar 

  • Jung, M. Y., Yoon, S. H., Lee, H. O., & Min, D. B. (1998). Singlet oxygen and ascorbic acid effects on dimethyl disulfide and off-flavor in skim milk exposed to light. Journal of Food Science, 63, 408–412.

    Article  CAS  Google Scholar 

  • Kamat, A. S., & Nair, P. M. (1996). Identification of Listeria innocua as a biological indicator for inactivation of L. monocytogenes by some meat processing treatments. LWT - Food Science and Technology, 29, 714–720.

    Article  CAS  Google Scholar 

  • Kondjoyan, N., & Berdagué, J. L. A. (1996). Compilation of relative retention indices for the analysis of aromatic compounds. Theix: Laboratoire Flaveur (INRA).

    Google Scholar 

  • Licón, C., Hurtado de Mendoza, J., Maggi, L., Berruga, M. I., Martín Aranda, R. M., & Carmona, M. (2012). Optimization of headspace sorptive extraction for the analysis of volatiles in pressed ewes’ milk cheese. International Dairy Journal, 23, 53–61.

    Article  Google Scholar 

  • Marcos, A., Fernández-Salguero, J., Esteban, M. A., León, F., Alcalá, M., & Beltrán de Heredia, F. H. (1985). Quesos españoles. Córdoba: Servicio de publicaciones de la Universidad de Córdoba.

    Google Scholar 

  • Mortensen, G., Bertelsen, G., Mortensen, B. K., & Stapelfeldt, H. (2004). Light-induced changes in packaged cheeses—a review. International Dairy Journal, 14, 85–102.

    Article  CAS  Google Scholar 

  • Proulx, J., Hsu, L. C., Miller, B. M., Sullivan, G., Paradis, K., & Moraru, C. I. (2015). Pulsed-light inactivation of pathogenic and spoilage bacteria on cheese surface. Journal of Dairy Science, 98, 5890–5898.

    Article  CAS  Google Scholar 

  • Rivas-Cañedo, A., Fernández-García, E., & Nuñez, M. (2009). Volatile compounds in dry-cured Serrano ham subjected to high pressure processing. Effect of the packaging material. Meat Science, 82, 162–169.

    Article  Google Scholar 

  • Rychlik, M., Schieberle, P., & Grosch, W. (1998). Compilation of odor thresholds, odor qualities and retention indices of key food odorants. Lebensmittelchemie and Institut für Lebensmittelchemie der Technischen Universität München. Germany: Deutsche Forschungsanstalt für Garching.

    Google Scholar 

  • Uesugi, A. R., & Moraru, C. I. (2009). Reduction of Listeria on ready-to-eat sausages after exposure to a combination of pulsed light and nisin. Journal of Food Protection, 72, 347–353.

    CAS  Google Scholar 

  • Walstra, P., Wouters, J. T. M., & Geurts, T. J. (2006). Dairy science and technology. Boca Raton, FL: CRC-Taylor & Francis.

    Google Scholar 

  • Woodling, S., & Moraru, C. (2005). Influence of surface topography on the effectiveness of pulsed light treatment for the inactivation of Listeria innocua on stainless-steel surface. Journal of Food Science, 70, 345–351.

    Article  Google Scholar 

  • WHO (2000). 7th report of the WHO surveillance programme for the control of foodborne infections and intoxications in Europe (1993-1998). FAO/WHO Collaborating Centre for Research and Training in Food Hygiene and Zoonoses. Rome: WHO European Centre for Environment and Health.

Download references

Acknowledgments

The authors gratefully acknowledge the support of the Ministerio de Economía y Competitividad (Government of Spain) through project AGL2011-29325.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Manuela Fernández.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fernández, M., Hospital, X.F., Arias, K. et al. Application of Pulsed Light to Sliced Cheese: Effect on Listeria Inactivation, Sensory Quality and Volatile Profile. Food Bioprocess Technol 9, 1335–1344 (2016). https://doi.org/10.1007/s11947-016-1721-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11947-016-1721-2

Keywords

Navigation