Skip to main content
SpringerLink
Log in

The Effect of High Power Ultrasound and Cold Gas-Phase Plasma Treatments on Selected Yeast in Pure Culture

  • Original Paper
  • Published:
Food and Bioprocess Technology Aims and scope Submit manuscript

Abstract

High power ultrasound (US) and cold gas-phase plasma (CP) are non-thermal processing technologies that maybe used in food processing industry. The main objective of this research was to study the effect of both treatments on selected yeasts (Rhodotorula spp. 74 and Candida spp. 86) in pure culture. Samples were treated by ultrasound with 57.50-, 86.25- or 115-μm amplitude, for 3, 6 or 9 min at 20 °C, and 40 or 60 °C in the case of thermosonication. For cold gas-phase plasma treatments, samples were treated at a gas flow of 0.75, 1 or 1.25 L min−1, treatment time of 3, 4 or 5 min, and sample volume of 2, 3 or 4 mL. Each technology has its own advantages and is able to give the best effect on the desired target product. The experiment was designed using central composite design (CCD), and results were analysed and presented using response surface methodology (RSM). The greatest reduction of yeasts was observed after ultrasound treatments at 60 °C (thermosonication) and after plasma treatments, after the longest treatment time (5 min) and the lowest sample volume (2 mL). For high power ultrasound treatment, reduction in the number of yeast cells (N) can be attributed to elevated temperature (60 °C), cavitation and free radical formation. For plasma treatment, the inactivation can be attributed to UV radiation and plasma reactive oxygen species (ROS).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ashokkumar, M., & Kentish, S. (2011). The physical and chemical effects of ultrasound. In H. Feng, G. Barobosa-Cànovas, & J. Weiss (Eds.), Ultrasound technologies for food and bioprocessing (pp. 1–105). New York: Springer.

    Google Scholar 

  • Bermudez-Aguirre, D., Mobbs, T., & Barbosa-Canovas, G. V. (2011). Ultrasound applications in food processing. In H. Feng, G. V. Barbosa-Canovas, & J. Weiss (Eds.), Ultrasound technologies for food and bioprocessing (pp. 65–105). USA: Springer.

    Chapter  Google Scholar 

  • Chemat, F., Zill-e-Huma, F., & Khan, M. K. (2011). Applications of ultrasound in food technology: processing, preservation and extraction. Ultrasonic Sonochemistry, 18, 813–835.

    Article  CAS  Google Scholar 

  • Herceg, Z., Režek Jambrak, A., Lelas, V., & Mededovic Thagard, S. (2012). The effect of high intensity ultrasound treatment on the amount of Staphylococcus aureus and Escherichia coli in milk. Food Technology and Biotechnology, 50, 46–52.

    CAS  Google Scholar 

  • Herceg, Z., Markov, K., Sobota Šalamon, B., Režek Jambrak, A., & Vukušić, T. (2013). Effect of high intensity ultrasound treatment on growth of food spoilage bacteria. Food Technology and Biotechnology, 51(3), 352–359.

    Google Scholar 

  • Hoffmann, M. R., Hua, I., & Höchemer, I. H. R. (1996). Application of ultrasonic irradiation for the degradation of chemical contaminants in water. Ultrasonics Sonochemistry, 3, 163–172.

    Article  Google Scholar 

  • Jambrak Režek, A., Mason, T. J., Lelas, V., Herceg, Z., & Ljubić Herceg, I. (2008). Effect of ultrasound treatment on solubility and foaming properties of whey protein suspensions. Journal of Food Engineering, 86(2), 281–287.

    Article  Google Scholar 

  • Jambrak Režek, A., Lelas, V., Mason, T. J., Krešić, G., & Badanjak, M. (2009). Physical properties of ultrasound treated soy proteins. Journal of Food Engineering, 93, 386–393.

    Article  Google Scholar 

  • Kamgang-Youbi, G., Herry, J. M., Meylheuc, T., Brisset, J. L., Bellon-Fontaine, M. N., et al. (2009). Microbial inactivation using plasma-activated water obtained by gliding electric discharges. Letters in Applied Microbiology, 48, 13–18.

    Article  CAS  Google Scholar 

  • Khuri, A. I., & Cornell, J. A. (1996). Response surfaces: design and analyses (2nd ed.). New York: Marcel Dekker.

    Google Scholar 

  • Kregar, Z., Bišćan, M., Milošević, S., & Vesel, A. (2011). Monitoring oxygen plasma treatment of polypropylene with optical emission spectroscopy. IEEE Transactions on Plasma Science, 39(5), 1239–1246.

    Article  CAS  Google Scholar 

  • Laroussi, M. (2005). Low temperature plasma-based sterilization: overview and state-of-the-art. Plasma Processes and Polymers, 2, 391–400.

    Article  CAS  Google Scholar 

  • Lee, K., Paek, K. H., Ju, W. T., & Lee, Y. (2006). Sterilization of bacteria, yeast, and bacterial endospores by atmospheric-pressure cold plasma using helium and oxygen. Journal of Microbiology, 44, 269–275.

    Google Scholar 

  • Lee, H., Zhou, B., Liang, W., Feng, H., & Martin, S. E. (2009). Inactivation of Escherichia coli cells with sonication, manosonication, thermosonication, and manothermosonication: microbial responses and kinetics modelling. Journal of Food Engineering, 93, 354–364.

    Article  Google Scholar 

  • Lu, C. H., Engelmann, N. J., Lila, M. A., & Erdman, J. W., Jr. (2008). Optimization of lycopene extraction from tomato cell suspension culture by response surface methodology. Journal of Agriculture and Food Chemistry, 56, 7710–7714.

    Article  CAS  Google Scholar 

  • Mason, T. J. (1998). Power ultrasound in food processing—the way forward. In M. J. W. Povey & T. J. Mason (Eds.), Ultrasound in food processing (pp. 103–126). London: Blackie Academic & Professional.

    Google Scholar 

  • Mason, T. J. (2003). Sonochemistry and sonoprocessing: the link, the trends and (probably) the future. Ultrasonic Sonochemistry, 10, 175–179.

    Article  CAS  Google Scholar 

  • Mitra, A., Li, Y. F., Klämpfl, T. G., Shimizu, T., Jeon, J., Morfill, G. E., & Zimmermann, J. L. (2014). Inactivation of surface-borne microorganisms and increased germination of seed specimen by cold atmospheric plasma. Food and Bioprocess Technology, 7, 645–653.

    Article  CAS  Google Scholar 

  • Muranyi, P., Wunderlich, J., & Heise, M. (2007). Sterilization efficiency of a cascaded dielectric barrier discharge. Journal of Applied Microbiology, 103, 1535–1544.

    Article  CAS  Google Scholar 

  • Myers, R. H., & Montgomery, D. C. (2002). Response surface methodology: process and product optimization using designed experiments (2nd ed.). USA: John Wiley & Sons.

    Google Scholar 

  • Peña-Eguiluz, R., Pérez-Martínez, J. A., Solís-Pacheco, J., Aguilar-Uscanga, B., López Callejas, R., Mercado-Cabrera, A., et al. (2010). Instrumentation for a plasma needle applied to E. coli bacteria elimination. The European Physical Journal Applied Physics, 49, 103–109.

    Article  Google Scholar 

  • Piyasena, P., Mohareb, E., & McKellar, R. C. (2003). Inactivation of microbes using ultrasound: a review. International Journal of Food Microbiology, 87, 207–216.

    Article  CAS  Google Scholar 

  • Raso, J., Pagan, R., Condon, S., & Sala, F. J. (1998). Influence of temperature on the lethality of ultrasound. Applied Environmental Microbiology, 64, 465–471.

    CAS  Google Scholar 

  • Roth, J. R., Fellow, L., Nourgostar, S., Member, S., Bonds, T. A., & Member, S. (2007). Plasma (OAUGDP)—a platform technology for the 21st century. IEEE Transactions on Plasma Science, 35, 233–250.

    Article  Google Scholar 

  • Ryu, Y.-H., Kim, Y.-H., Lee, J.-Y., Shim, G.-B., Uhm, H.-S., et al. (2013). Effects of background fluid on the efficiency of inactivating yeast with non-thermal atmospheric pressure plasma. Public Library of Science (PLOS). PLoS ONE, 8(6), e66231. doi:10.1371/journal.pone.0066231.

    Article  CAS  Google Scholar 

  • Salleh-Mack, S. Z., & Roberts, J. S. (2007). Ultrasound pasteurization: the effects of temperature soluble solids organic acids and pH on the inactivation of Escherichia coli ATCC 25922. Ultrasonic Sonochemistry, 14, 323–329.

    Article  CAS  Google Scholar 

  • Sun, Y., Yu, S., Sun, P., Wu, H., Zhu, W., et al. (2012). Inactivation of Candida biofilms by non-thermal plasma and its enhancement for fungistatic effect of antifungal drugs. Public Library of Science (PLOS). PLoS ONE, 7(7), e40629. doi:10.1371/journal.pone.0040629.

    Article  CAS  Google Scholar 

  • Šimunek, M., Jambrak Režek, A., Petrović, M., Juretić, H., Major, N., Herceg, Z., Hruškar, M., & Vukušić, T. (2013). Aroma profile and sensory properties of ultrasound-treated apple juice and nectar. Food Technology and Biotechnology, 51(1), 101–111.

    Google Scholar 

  • Tang, Y. Z., Lu, X. P., Laroussi, M., & Dobbs, F. C. (2008). Sublethal and killing effects of atmospheric-pressure, nonthermal plasma on eukaryotic microalgae in aqueous media. Plasma Processes and Polymers, 5, 552–558.

    Article  CAS  Google Scholar 

  • Vleugels, M., Shama, G., Deng, X. T., Greenacre, E., Brocklehurst, T., & Kong, M. G. (2005). Atmospheric plasma inactivation of biofilm-forming bacteria for food safety control. IEEE Transactions on Plasma Science, 33, 824.

    Article  CAS  Google Scholar 

  • Xiong, Z., Lu, X. P., Feng, A., Pan, Y., & Ostrikov, K. (2010). Highly effective fungal inactivation in He + O2 atmospheric-pressure nonequilibrium plasmas. Physics of Plasmas, 17, 123502.

    Article  Google Scholar 

  • Zaplotnik, R., Kregar, Z., Bišćan, M., Vesel, A., Cvelbar, U., Mozetič, M., & Milošević, S. (2014). Multiple vs. single harmonics AC-driven atmospheric plasma jet. EPL, 106, 25001.

    Article  Google Scholar 

Download references

Acknowledgments

This work has been supported in part by the Croatian Science Foundation under the project IP-11-2013-6248 “Application of electrical discharge plasma for preservation of liquid foods.”

Author information

Authors and Affiliations

  1. Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia

    Anet Režek Jambrak, Tomislava Vukušić, Višnja Stulić, Jasna Mrvčić, Marina Šimunek & Zoran Herceg

  2. Institute of Physics, Bijenička 46, 10000, Zagreb, Croatia

    Slobodan Milošević

Authors
  1. Anet Režek Jambrak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tomislava Vukušić
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Višnja Stulić
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jasna Mrvčić
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Slobodan Milošević
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Marina Šimunek
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zoran Herceg
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zoran Herceg.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jambrak, A.R., Vukušić, T., Stulić, V. et al. The Effect of High Power Ultrasound and Cold Gas-Phase Plasma Treatments on Selected Yeast in Pure Culture. Food Bioprocess Technol 8, 791–800 (2015). https://doi.org/10.1007/s11947-014-1442-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11947-014-1442-3

Keywords

Search

Navigation