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The Effect of Endogenous Pectinases on the Consistency of Tomato–Carrot Purée Mixes

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Abstract

Tomato and carrot were subjected to a split-stream process designed to produce a tomato–carrot suspension with reduced consistency. Raw tomatoes, containing pectinmethylesterase and endo-polygalacturonase, were mixed with thermally pretreated (blanched versus cooked) carrots containing different levels of solubilized pectin. After mixing the vegetables, tomato pectinases were shown to act on both tomato and carrot pectin in case an incubation step at medium temperature level (30 min, 40 °C), to allow enzyme action, was performed. Carrot pectin, when present in a mix of tomato and blanched (5 min, 95 °C) carrot, was solubilized as well as depolymerized, whereas depolymerization of the thermo-solubilized carrot pectin by the tomato pectinases was observed in the tomato–carrot purée containing cooked (30 min, 95 °C) carrots. The final serum pectin properties were however similar for both purée types. Carrot contributed more to the consistency of the purée mix compared with tomato but by stimulating the action of the tomato pectinases at mild temperature (30 min, 40 °C), this contribution was lost which resulted in a consistency reduction of the purée mix. This purée liquefaction was larger for the tomato–carrot purée containing blanched instead of cooked carrots. Based on the results, it is suggested that the liquefying effect is related to solubilization and degradation of pectin that is counteracted by a reduction in particle size. The purée mix containing cooked carrot showed in this respect smaller particle sizes than the mix containing blanched carrot.

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Acknowledgments

This research has been carried out with financial support from the Research Fund KU Leuven (KP/08/004) and from the Agency for Innovation by Science and Technology in Flanders (IWT-Vlaanderen). S. Christiaens and S. Van Buggenhout are postdoctoral researchers, funded by the Research Foundation Flanders (FWO).

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Correspondence to Marc E. Hendrickx.

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Houben, K., Christiaens, S., Ngouémazong, D.E. et al. The Effect of Endogenous Pectinases on the Consistency of Tomato–Carrot Purée Mixes. Food Bioprocess Technol 7, 2570–2580 (2014). https://doi.org/10.1007/s11947-014-1284-z

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