Abstract
Tomato and carrot were subjected to a split-stream process designed to produce a tomato–carrot suspension with reduced consistency. Raw tomatoes, containing pectinmethylesterase and endo-polygalacturonase, were mixed with thermally pretreated (blanched versus cooked) carrots containing different levels of solubilized pectin. After mixing the vegetables, tomato pectinases were shown to act on both tomato and carrot pectin in case an incubation step at medium temperature level (30 min, 40 °C), to allow enzyme action, was performed. Carrot pectin, when present in a mix of tomato and blanched (5 min, 95 °C) carrot, was solubilized as well as depolymerized, whereas depolymerization of the thermo-solubilized carrot pectin by the tomato pectinases was observed in the tomato–carrot purée containing cooked (30 min, 95 °C) carrots. The final serum pectin properties were however similar for both purée types. Carrot contributed more to the consistency of the purée mix compared with tomato but by stimulating the action of the tomato pectinases at mild temperature (30 min, 40 °C), this contribution was lost which resulted in a consistency reduction of the purée mix. This purée liquefaction was larger for the tomato–carrot purée containing blanched instead of cooked carrots. Based on the results, it is suggested that the liquefying effect is related to solubilization and degradation of pectin that is counteracted by a reduction in particle size. The purée mix containing cooked carrot showed in this respect smaller particle sizes than the mix containing blanched carrot.
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References
Ahmed, A. E. R., & Labavitch, J. M. (1977). A simplified method for accurate determination of cell wall uronide content. Journal of Food Biochemistry, 1, 361–365.
Assinder, I., Supran, M. K., & Thompson, G. A. K. (1987). Food processing method to avoid non-enzymatic browning.US 06/731,572(US4640840).
Blumenkrantz, N., & Asboe-Hansen, G. (1973). New method for quantitative determination of uronic acids. Analytical Biochemistry, 54(2), 484–489.
Christiaens, S., Van Buggenhout, S., Chaula, D., Moelants, K., David, C. C., & Hofkens, J. (2012a). In situ pectin engineering as a tool to tailor the consistency and syneresis of carrot purée. Food Chemistry, 133(1), 146–155.
Christiaens, S., Van Buggenhout, S., Houben, K., Chaula, D., Van Loey, A. M., & Hendrickx, M. E. (2012b). Unravelling process-induced pectin changes in the tomato cell wall: an integrated approach. Food Chemistry, 132(3), 1534–1543.
Houben, K., Jamsazzadeh Kermani, Z., Van Buggenhout, S., Jolie, R. P., Van Loey, A. M., & Hendrickx, M. E. (2013a). Thermal and high-pressure stability of pectinmethylesterase, polygalacturonase, β-galactosidase and α-arabinofuranosidase in a tomato matrix: towards the creation of specific endogenous enzyme populations through processing. Food and Bioprocess Technology, 6(12), 3368–3380.
Houben, K., Jamsazzadeh Kermani, Z., Van Buggenhout, S., Van Loey, A. M., & Hendrickx, M. E. (2013b). Thermal and high-pressure stability of pectin-converting enzymes in broccoli and carrot purée: towards the creation of specific endogenous enzyme populations through processing. Food and Bioprocess Technology. doi:10.1007/s11947-013-1166-9.
Lopez-Sanchez, P., Nijsse, J., Blonk, H. C. G., Bialek, L., Schumm, S., & Langton, M. (2011a). Effect of mechanical and thermal treatments on the microstructure and rheological properties of carrot, broccoli and tomato dispersions. Journal of the Science of Food and Agriculture, 91(2), 207–217.
Lopez-Sanchez, P., Svelander, C., Bialek, L., Schumm, S., & Langton, M. (2011b). Rheology and microstructure of carrot and tomato emulsions as a result of high-pressure homogenization conditions. Journal of Food Science, 76(1), E130–E140.
Miller, R. A. (1965). Process for preparing tomato concentrate. (US3172770 A).
Moelants, K. R. N., Cardinaels, R., Jolie, R. P., Verrijssen, T. A. J., Van Buggenhout, S., Van Loey, A. M., Moldenaers, P., & Hendrickx, M. E. (2013). Rheology of concentrated tomato-derived suspensions: effects of particle characteristics. Food and Bioprocess Technology. doi:10.1007/s11947-013-1070-3.
Moelants, K. R. N., Jolie, R. P., Palmers, S. K. J., Cardinaels, R., Christiaens, S., Van Buggenhout, S., Van Loey, A. M., Moldenaers, P., & Hendrickx, M. E. (2012). The effects of process-induced pectin changes on the viscosity of carrot and tomato sera. Food and Bioprocess Technology. doi:10.1007/s11947-012-1004-5.
Svelander, C. A., Lopez-Sanchez, P., Pudney, P. D. A., Schumm, S., & Alminger, M. A. G. (2011). High pressure homogenization increases the in vitro bioaccessibility of α- and β-carotene in carrot emulsions but not of lycopene in tomato emulsions. Journal of Food Science, 76(9), H215–H225.
Van Buggenhout, S., Ahrne, L., Alminger, M., Andrys, A., Benjamin, M., Bialek, L., Cleaver, G., Colle, I., Langton, M., Larque, E., Lemmens, L., Lofgren, A., Lopez-Sanchez, P., Perez-Llamas, F., Martinez-Tomas, R., Robertson, J., Schalow, S., Svelander, C., Wellner, N., Hendrickx, M., & Waldron, K. (2012). Structural design of natural plant-based foods to promote nutritional quality. Trends in Food Science & Technology, 24(1), 47–59.
Verlent, I., Hendrickx, M., Rovere, P., Moldenaers, P., & Van Loey, A. (2006). Rheological properties of tomato-based products after thermal and high-pressure treatment. Journal of Food Science, 71(3), S243–S248.
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This research has been carried out with financial support from the Research Fund KU Leuven (KP/08/004) and from the Agency for Innovation by Science and Technology in Flanders (IWT-Vlaanderen). S. Christiaens and S. Van Buggenhout are postdoctoral researchers, funded by the Research Foundation Flanders (FWO).
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Houben, K., Christiaens, S., Ngouémazong, D.E. et al. The Effect of Endogenous Pectinases on the Consistency of Tomato–Carrot Purée Mixes. Food Bioprocess Technol 7, 2570–2580 (2014). https://doi.org/10.1007/s11947-014-1284-z
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DOI: https://doi.org/10.1007/s11947-014-1284-z