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Effects of Hydrolysates from Silver Carp (Hypophthalmichthys molitrix) Scales on Rancidity Stability and Gel Properties of Fish Products

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Abstract

This study aimed to evaluate the effectiveness of hydrolysates, which were obtained from the scales of silver carp (Hypophthalmichthys molitrix) by papain, flavourzyme, and Alcalase 2.4 L, as natural antioxidants in silver carp mince and surimi gels during storage at 4 °C. The hydrolysates that possess greater in vitro antioxidant activities (DPPH radical-scavenging activity, Fe2+-chelating activity, and reducing power), including hydrolysates catalyzed by papain at 10 min (HP), flavourzyme at 5 min (HF), and Alcalase 2.4 L at 5 min (HA), were chosen as additives. Color, cooking loss, conjugated dienes (CDs), thiobarbituric acid reactive substances (TBARS), fatty acids, and sensory scores of mince were measured on days 0, 2, 4, 6, and 8 during 4 °C storage; additionally, whiteness, breaking force, deformation, gel strength, and sensory score of surimi gels were measured on days 1, 3, 5, 7, 9, and 11 during 4 °C storage. The results indicate that HA was conducive to lowering the cooking loss of mince and that HF significantly (P < 0.05) reduced the CDs value of mince. For surimi gels, HF improved whiteness, deformation, and gel strength. Hence, HF could serve as a natural antioxidant during early oxidation and improve gel formation of silver carp products.

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References

  • Alishahi, A., & Aïder, M. (2012). Applications of chitosan in the seafood industry and aquaculture: a review. Food and Bioprocess Technology, 5, 817–830.

    Article  CAS  Google Scholar 

  • AOAC International. (2002). Official methods of analysis (17th ed.). Gaithersburg: Association of Official Analytical Chemists.

    Google Scholar 

  • Benjakula, S., Visessanguanb, W., & Tueksubana, J. (2003). Changes in physico-chemical properties and gel-forming ability of lizardfish (Saurida tumbil) during post-mortem storage in ice. Food Chemistry, 80, 535–544.

    Article  Google Scholar 

  • Cardoso, C. L., Mendes, R. O., Saraiva, J. A., Vaz-Pires, P., & Nunes, M. L. (2010). Quality characteristics of high pressure-induced hake (Meluccius capensis) protein gel with and without MTGase. Journal of Aquatic Food Product Technology, 19(3–4), 193–213.

    Article  CAS  Google Scholar 

  • Careche, M., & Li-Chan, E. (1997). Structural changes and Raman spectroscopic studies of cod myosin upon modification with formaldehyde or frozen storage. Journal of Food Science, 62, 717–723.

    Article  CAS  Google Scholar 

  • Chen, Y. C. (2012). The biomass and total lipid content and composition of twelve species of marine diatoms cultured under various environments. Food Chemistry, 131, 211–219.

    Google Scholar 

  • Decker, E. A., & Díaz, M. (2004). Antioxidant mechanisms of caseinophosphopeptides and casein hydrolysates and their application in ground beef. Journal of Agriculture and Food Chemistry, 52, 8208–8213.

    Article  Google Scholar 

  • Decker, E. A., & Welch, B. (1990). Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agriculture and Food Chemistry, 38, 674–677.

    Article  CAS  Google Scholar 

  • Decker, E. A., Warner, K., Richards, M. P., & Shahidi, F. (2005). Measuring antioxidant effectiveness in food. Journal of Agriculture and Food Chemistry, 53, 4303–4310.

    Article  CAS  Google Scholar 

  • Erkan, N., & Özden, Ö. (2008). Quality assessment of whole and gutted sardines (Sardina pilchardus) stored in ice. International Journal of Food Science & Technology, 43(9), 1549–1559.

    Article  CAS  Google Scholar 

  • Folch, J., Lees, M., & Stanly, G. H. S. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226, 497–509.

    CAS  Google Scholar 

  • Hong, H., Luo, Y. K., Zhou, Z. Y., & Shen, H. X. (2012). Effects of low concentration of salt and sucrose on the quality of bighead carp (Aristichthys nobilis) fillets stored at 4 °C. Food Chemistry, 133, 102–107.

    Article  CAS  Google Scholar 

  • Iglesias-Otero, M. A., Borderías, J., & Tovar, C. A. (2010). Use of Konjac glucomannan as additive to reinforce the gels from low-quality squid surimi. Journal of Food Engineering, 101(3), 281–288.

    Article  CAS  Google Scholar 

  • Khantaphant, S., Benjakul, S., & Ghomi, M. R. (2011). The effects of pretreatments on antioxidative activities of protein hydrolysate from the muscle of brownstripe red snapper (Lutjanus vitta). LWT - Food Science and Technology, 44, 1139–1148.

    Article  CAS  Google Scholar 

  • Kudre, T., Benjakul, S., & Kishimura, H. (2012). Effects of protein isolates from black bean and mungbean on proteolysis and gel properties of surimi from sardine (Sardinella albella). LWT - Food Science and Technology, 50(2), 511–518.

    Article  Google Scholar 

  • Li, X., Luo, Y. K., You, J., Shen, H. X., & Du, J. (2012). In vitro antioxidant activity of papain-treated grass carp (Ctenopharyngodon idellus) protein hydrolysate and the preventive effect on fish mince system. International Journal of Food Science and Technology, 47, 961–967.

    Article  CAS  Google Scholar 

  • Luo, Y. K., Shen, H. X., Pan, D. D., & Bu, G. H. (2008). Gel properties of surimi from silver carp (Hypophthalmichthys molitrix) as affected by heat treatment and soy protein isolate. Food Hydrocolloids, 22(8), 1513–1519.

    Article  CAS  Google Scholar 

  • Paari, A., Naidu, H. K., Kanmani, P., Satishkumar, R., Yuvaraj, N., Pattukumar, V., et al. (2012). Evaluation of irradiation and heat treatment on antioxidant properties of fruit peel extracts and its potential application during preservation of goat fish Parupenaeus indicus. Food and Bioprocess Technology, 5, 1860–1870.

    Article  CAS  Google Scholar 

  • Pan, J. F., Shen, H. X., & Luo, Y. K. (2010). Cryoprotective effects of trehalose on grass carp (Ctenopharyngodon idellus) surimi during frozen storage. Journal of Food Processing and Preservation, 34(4), 715–727.

    CAS  Google Scholar 

  • Park, J. W. (1994). Functional protein additives in surimi gels. Journal of Food Science, 59, 525–527.

    Article  CAS  Google Scholar 

  • Penã-Ramos, E. A., & Xiong, Y. L. (2003). Whey and soy protein hydrolysates inhibit lipid oxidation in cooked pork patties. Food Chemistry, 64, 259–263.

    Google Scholar 

  • Phakawat, T., Soottawat, B., & Prodpran, T. (2011). Roles of lipid oxidation and pH on properties and yellow discolouration during storage of film from red tilapia (Oreochromis niloticus) muscle protein. Food Hydrocolloids, 25, 426–433.

    Article  Google Scholar 

  • Raghavan, S., & Kristinsson, H. G. (2008). Antioxidative efficacy of alkali-treated tilapia protein hydrolysates: a comparative study of five enzymes. Journal of Agricultural and Food Chemistry, 56(4), 1434–1441.

    Article  CAS  Google Scholar 

  • Sikorski, Z., Kołakowska, A., & Burt, J. R. (1990). Postharvest biochemical and microbial changes. In Z. E. Sikorski (Ed.), Seafood: Resources, nutritional composition and preservation (pp. 55–75). Boca Raton: CRC.

    Google Scholar 

  • Theodore, A. E., Raghavan, S., & Kristinsson, H. G. (2008). Antioxidative activity of protein hydrolysates prepared from alkaline-aided channel catfish protein isolates. Journal of Agricultural and Food Chemistry, 56(16), 7459–7466.

    Article  CAS  Google Scholar 

  • Wang, L. L., & Xiong, Y. L. (2005). Inhibition of lipid oxidation in cooked beef patties by hydrolyzed potato protein is related to its reducing and radical scavenging ability. Journal of Agriculture and Food Chemistry, 53, 9186–9192.

    Article  CAS  Google Scholar 

  • Yildirim, A., Mavi, A., & Kara, A. A. (2001). Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. Journal of Agriculture and Food Chemistry, 49, 4083–4089.

    Article  CAS  Google Scholar 

  • Yin, M. C., & Faustman, C. (1993). The influence of temperature, pH, and phospholipid composition upon the stability of myoglobin and phospholipid: a liposome model. Journal of Agricultural and Food Chemistry, 41, 853–857.

    Article  CAS  Google Scholar 

  • Zuta, P. C., Simpson, B. K., Zhao, X., & Leclerc, L. (2007). The effect of a-tocopherol on the oxidation of mackerel oil. Food Chemistry, 100, 800–807.

    Article  CAS  Google Scholar 

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Acknowledgments

This study was supported by the earmarked fund for China Agriculture Research System (CARS-46).

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Correspondence to Yongkang Luo.

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Lu, H., Luo, Y. & Feng, L. Effects of Hydrolysates from Silver Carp (Hypophthalmichthys molitrix) Scales on Rancidity Stability and Gel Properties of Fish Products. Food Bioprocess Technol 7, 2178–2188 (2014). https://doi.org/10.1007/s11947-013-1196-3

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  • DOI: https://doi.org/10.1007/s11947-013-1196-3

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