Abstract
Lactobacillus rhamnosus GG (LGG) and Lactobacillus acidophilus NCFM (LNCFM) were encapsulated in alginate microgel particles (microbeads) by a novel dual aerosols method. The encapsulated probiotics in microbead gel matrix were further stabilized in maltodextrin solids by either spray or freeze-drying to form probiotic microcapsule powders. The free cells of probiotics were also sprayed and freeze-dried in maltodextrin only without microgel encapsulation. After rehydration of microgel-encapsulated powder, gel particles regained their shape. There was no difference in the loss of viability between encapsulated and unencapsulated probiotics during spray drying or freeze-drying. For LNCFM, spray-dried bacteria with or without gel encapsulation exhibited less death (3.03 and 3.07 log CFU/g reduction, respectively) than those of freeze-dried bacteria (4.36 and 4.89 log CFU/g reduction, respectively) after 6 months storage at 4 °C. The same trend was also observed in spray-dried LGG without gel encapsulation which showed 5.87 log CFU/g reduction in viability after 6 months at 4 °C; however, freeze-dried LGG without gel encapsulation exhibited a rapid reduction in viability of 5.91 log CFU/g within just 2 months. Gel-encapsulated LGG which was freeze-dried exhibited less death (3.32 log CFU/g reduction) after 6 months at 4 °C. This work shows that spray drying results in improved subsequent probiotic survivability compared to freeze-drying and that alginate gel encapsulation can improve the survivability following freeze-drying in a probiotic-dependent manner.
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References
Anal, A. K., & Singh, H. (2007). Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery. Trends in Food Science & Technology, 18(5), 240–251.
Ananta, E., Volkert, M., & Knorr, D. (2005). Cellular injuries and storage stability of spray-dried Lactobacillus rhamnosus GG. International Dairy Journal, 15(4), 399–409.
AOAC. (2008). Official methods of analysis (18th ed.). Washington: Association of Official Analytical Chemists.
Capela, P., Hay, T. K. C., & Shah, N. P. (2006). Effect of cryoprotectants, prebiotics and microencapsulation on survival of probiotic organisms in yoghurt and freeze-dried yoghurt. Food Research International, 39(2), 203–211.
Champagne, C. P., Gardner, N. J., & Roy, D. (2005). Challenges in the addition of probiotic cultures to foods. Critical Reviews in Food Science and Nutrition, 45(1), 61–84.
Chavez, B. E., & Ledeboer, A. M. (2007). Drying of probiotics: optimization of formulation and process to enhance storage survival. Drying Technology, 25(7–8), 1193–1201.
Collins, J. K., Thornton, G., & Sullivan, G. O. (1998). Selection of probiotic strains for human applications. International Dairy Journal, 8(5–6), 487–490.
De Giulio, B., Orlando, P., Barba, G., Coppola, R., De Rosa, M., Sada, A., et al. (2005). Use of alginate and cryo-protective sugars to improve the viability of lactic acid bacteria after freezing and freeze-drying. World Journal of Microbiology and Biotechnology, 21(5), 739–746.
Gibbs, B. F., Kermasha, S., Alli, I., & Mulligan, C. N. (1999). Encapsulation in the food industry: a review. International Journal of Food Sciences and Nutrition, 50(3), 213–224.
Goderska, K., & Czarnecki, Z. (2008). Influence of micro encapsulation and spray drying on the viability of Lactobacillus and Bifidobacterium strains. Polish Journal of Microbiology, 57(2), 135–140.
Hamsupo, K., Sukyai, P., Nitisinprasert, S., & Wanchaitanawong, P. (2005). Different growth media and growth phases affecting on spray drying and freeze drying of Lactobacillus reuteri KUB-AC5. Kasetsart Journal (Natural Science), 39(4), 718–724.
Krasaekoopt, W., Bhandari, B., & Deeth, H. (2004). The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria. International Dairy Journal, 14(8), 737–743.
Li, X. Y., Chen, X. G., Cha, D. S., Park, H. J., & Liu, C. S. (2009). Microencapsulation of a probiotic bacteria with alginate–gelatin and its properties. Journal of Microencapsulation, 26(4), 315–324.
Manojlovic', V., Nedovic', V. A., Kailasapathy, K., & Zuidam, N. J. (2010). Encapsulation of probiotics for use in food products. In N. J. Zuidam & V. A. Nedovic (Eds.), Encapsulation technologies for active food ingredients and food processing (pp. 269–302). New York: Springer.
Mokarram, R. R., Mortazavi, S. A., Najafi, M. B. H., & Shahidi, F. (2009). The influence of multi stage alginate coating on survivability of potential probiotic bacteria in simulated gastric and intestinal juice. Food Research International, 42(8), 1040–1045.
Morelli, L. (2000). In vitro selection of probiotic lactobacilli: a critical appraisal. Current Issues in Intestinal Microbiology, 1(2), 59–67.
Picot, A., & Lacroix, C. (2004). Encapsulation of bifidobacteria in whey protein-based microcapsules and survival in simulated gastrointestinal conditions and in yoghurt. International Dairy Journal, 14(6), 505–515.
Riveros, B., Ferrer, J., & Borquez, R. (2009). Spray drying of a vaginal probiotic strain of Lactobacillus acidophilus. Drying Technology, 27(1), 123–132.
Rokka, S., & Rantamaki, P. (2010). Protecting probiotic bacteria by microencapsulation: challenges for industrial applications. European Food Research and Technology, 231(1), 1–12.
Semyonov, D., Ramon, O., Kaplun, Z., Levin-Brener, L., Gurevich, N., & Shimoni, E. (2010). Microencapsulation of Lactobacillus paracasei by spray freeze drying. Food Research International, 43(1), 193–202.
Shilpa, A., Agrawal, S. S., & Ray, A. R. (2003). Controlled delivery of drugs from alginate matrix. Polymer Reviews, 43(2), 187–221.
Sohail, A., Turner, M. S., Coombes, A., Bostrom, T., & Bhandari, B. (2011). Survivability of probiotics encapsulated in alginate gel microbeads using a novel impinging aerosols method. International Journal of Food Microbiology, 145(1), 162–168.
Sohail, A., Turner, M. S., Prabawati, E. K., Coombes, A. G. A., & Bhandari, B. (2012). Evaluation of Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM encapsulated using a novel impinging aerosol method in fruit food products. International Journal of Food Microbiology, 157(2), 162–166.
Teixeira, P., Castro, H., & Kirby, R. (1995). Spray drying as a method for preparing concentrated cultures of Lactobacillus bulgaricus. Journal of Applied Microbiology, 78(4), 456–462.
Teixeira, P. C., Castro, M. H., Malcata, F. X., & Kirby, R. M. (1995). Survival of Lactobacillus delbrueckii ssp. bulgaricus following spray-drying. Journal of Dairy Science, 78(5), 1025–1031.
Teixeira, P., Castro, H., & Kirby, R. (1996). Evidence of membrane lipid oxidation of spray-dried Lactobacillus bulgaricus during storage. Letters in Applied Microbiology, 22(1), 34–38.
Wang, Y. C., Yu, R. C., & Chou, C. C. (2004). Viability of lactic acid bacteria and bifidobacteria in fermented soymilk after drying, subsequent rehydration and storage. International Journal of Food Microbiology, 93(2), 209–217.
Ying, D. Y., Phoon, M. C., Sanguansri, L., Weerakkody, R., Burgar, I., & Augustin, M. A. (2010). Microencapsulated Lactobacillus rhamnosus GG powders: relationship of powder physical properties to probiotic survival during storage. Journal of Food Science, 75(9), E588–E595.
Zhou, Y., Martins, E., Groboillot, A., Champagne, C. P., & Neufeld, R. J. (1998). Spectrophotometric quantification of lactic bacteria in alginate and control of cell release with chitosan coating. Journal of Applied Microbiology, 84(3), 342–348.
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Sohail, A., Turner, M.S., Coombes, A. et al. The Viability of Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM Following Double Encapsulation in Alginate and Maltodextrin. Food Bioprocess Technol 6, 2763–2769 (2013). https://doi.org/10.1007/s11947-012-0938-y
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DOI: https://doi.org/10.1007/s11947-012-0938-y