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Effects of Liquid Whey Protein Concentrate on Functional and Sensorial Properties of Set Yogurts and Fresh Cheese

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Abstract

The production and incorporation of liquid whey protein concentrates (LWPCs) in fresh cheese and set yogurt is proposed as a solution for immediate reutilization of whey produced by small- and medium-scale dairy plants avoiding expensive processing steps (e.g., evaporation and drying) for the recovery of this by-product. Accordingly, the incorporation of LWPCs in such products was performed in order to compare the functional and sensorial properties of modified products with the conventional ones. The use of LWPC in fresh cheese increased water-holding capacity as well as product stability during storage. Fresh cheese hardness, chewiness, and gumminess decreased during storage in a more pronounced way in products with LWPC. The fat content influences significantly all the physicochemical properties tested in set yogurts. Incorporating LWPC in set yogurts does not produce appreciable differences in the visual properties when considering products with medium-fat content, but these differences become significant for full-fat yogurts. Adhesiveness and springiness were not significantly affected with storage time or by the amount of LWPC incorporated for medium-fat yogurts. Higher values of hardness and gumminess were obtained for full-fat yogurts, but these parameters decreased with LWPC incorporation. Syneresis was reduced using LWPC but increased with storage time. During storage, viscosity differences between LWPC incorporated yogurts and the conventional ones were only maintained in the case of creamy yogurts. The sensory panel detected differences between conventional and modified products in the case of fresh cheeses but no significative differences were detected between yogurts. LWPCs can be a good alternative to conventional dry products used in fresh cheese and set yogurt manufacture since their utilization reduces milk consumption and allows for the increase in total solids content. Additionally, their incorporation in milk originates end products with attractive physicochemical and sensorial characteristics at lower production costs.

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References

  • Abrahamsen, R. K. (1979). Cheese making from milk fortified with ultrafiltrated whey protein concentrate. Milchwissenschaft, 34(29), 1–4.

    Google Scholar 

  • Amatayakul, T., Sherkat, & Shah, N. P. (2006). Syneresis in set yogurt as affected by EPS starter cultures and levels of solids. International Journal of Dairy Technology, 59, 216–221.

    Article  CAS  Google Scholar 

  • AOAC (1997) Official methods of analysis of Association of Official Analytical Chemists. 16th ed. Volume II. 33 Dairy Products USA.

  • Baldwin, K. A., Baer, R. J., Parsons, J. G., Seas, S. W., Spurgeon, K. R., & Torrey, J. S. (1986). Evaluation of yield and quality of cheddar cheese manufactured from milk with added whey protein concentrate. Journal of Dairy Science, 69, 2543–2550.

    Article  CAS  Google Scholar 

  • Banks, J. M., & Muir, D. D. (1985). Effect of incorporation of denatured whey protein on the yield and quality of cheddar cheese. Journal Society of Dairy Technology, 38, 27–32.

    Article  Google Scholar 

  • Cais-Sokolinska, D., & Pikul, J. (2006). Use of colour measurement to evaluate yoghurt quality during storage. Italian Journal of Food Science, 1(18), 63–71.

    Google Scholar 

  • Damin, M. R., Alcântara, M. R., Nunes, A. P., & Oliveira, M. N. (2009). Effects of milk supplementation with skim milk powder, whey protein concentrate and sodium caseinate on acidification kinetics, rheological properties and structure of nonfat stirred yogurt. LWT—Food Science and Technology, 42, 1744–1750.

    CAS  Google Scholar 

  • Diaz, O., Pereira, C. D., & Cobos, A. (2009). Aplicaciones de los concentrados y aislados de proteínas de lactosuero de la industria alimentaria. Alimentaria—Investigacion, Tecnologia e Seguridad, 400, 108–115.

    Google Scholar 

  • Gauche, C. (2007). Polimerização de proteínas do soro de leite por transglutaminase e propriedades físicas de iogurte elaborado após tratamento enzimático. Florianópolis, Brasil: Pós-Graduação em Ciência dos Alimentos.

    Google Scholar 

  • Gomes, D. (2010). Incorporação de concentrados líquidos de proteínas de soro em produtos lácteos. Escola Superior Agrária de Coimbra. Master thesis, College of Agriculture—Polytechnic Institute of Coimbra, Coimbra, Portugal.

  • Guzmán-González, M., Morais, F., Ramos, M., & Amigo, L. (1999). Influence of skimmed milk concentrate replacement by dry dairy products in a low fat set-type yoghurt model system. I: Use of whey protein concentrates, milk protein concentrates and skimmed milk powder. Journal of the Science of Food and Agriculture, 79, 1117–1122.

    Article  Google Scholar 

  • Harwalkar, V. R., & Kalab, M. (1986). Relationship between microstructure and susceptibility to whey separation in yoghurt made from reconstituted nonfat dry milk. Food Microstructure, 5, 287–294.

    Google Scholar 

  • Herrero, A. M., & Requena, T. (2006). The effect of supplementing goat's milk with whey protein concentrate on textural properties of set-type yoghurt. International Journal of Food Science and Technology, 41, 87–92.

    Article  CAS  Google Scholar 

  • Hill, T., & Lewicki, P. (2007). Statistics Methods and Applications. Tulsa, OK: StatSoft.

    Google Scholar 

  • Hinrichs, J. (2001). Incorporation of whey proteins in cheese. International Dairy Journal, 11, 495–503.

    Article  CAS  Google Scholar 

  • IPQ. (1990). Leite e Produtos lácteos. Lisboa, Portugal: Edição Normas Portuguesas.

    Google Scholar 

  • ISO 8586-2. (1994). Sensory analysis—General guidance for the selection, training and monitoring of assessors (Part 2: Experts) (1st ed.).

    Google Scholar 

  • Jameson, G. W., & Lelievre, J. (1996). Effects of whey proteins on cheese characteristics. Bulletin of the IDF, 313, 3–8.

    CAS  Google Scholar 

  • Jaros, D., Rohm, H., Haque, A., & Kneifel, W. (2002). Influence of the starter cultures on the relationship between dry matter content and physical properties of set-style yogurt. Milchwissenschaft, 57, 325–326.

    CAS  Google Scholar 

  • Jorge, C., Lobete, N. & Pereira, C. (2006). Adição de proteínas de soro desnaturadas no fabrico de queijo. Leite I + D + T. N.º 0, 2–4.

  • Kailasapathy, K., Supriadi, D., & Hourigan, J. A. (1996). Effect of partially replacing skim milk powder with whey protein concentrate on buffering capacity of yoghurt. Australian Journal of Dairy Technology, 51, 89–93.

    CAS  Google Scholar 

  • Katsiari, M. C., Voutsinas, L. P., & Kondyli, E. (2002). Manufacture of yoghurt from stored frozen sheep's milk. Food Chemistry, 77, 413–420.

    Article  CAS  Google Scholar 

  • Korolczuk, J., & Mahaut, M. (1991a). Effect of whey proteins and heat treatment of milk on the consistency of UF fresh cheese. Milchwissenschaft, 46, 435–437.

    CAS  Google Scholar 

  • Korolczuk, J., & Mahaut, M. (1991b). Consistency of acid fresh cheese. Role of whey proteins. Milchwissenschaft, 46, 153–156.

    CAS  Google Scholar 

  • Lebeuf, Y., Lacroix, C., & Paquin, P. (1998). Effet de l'incorporation des protéines du lactosérum dénaturées et microparticules dans le cheddar jeune. Le Lait, 78(3), 303–318.

    Article  CAS  Google Scholar 

  • Lee, W. J., & Lucey, J. A. (2010). Formation and physical properties of yogurt. Asian-Australasian Journal of Animal Sciences, 23(9), 1127–1136.

    Article  CAS  Google Scholar 

  • Li, J., & Guo, M. (2006). Effects of polymerized whey proteins on consistency and water-holding properties of goat's milk yogurt. Journal of Food Science, 71(1), 34–38.

    Article  Google Scholar 

  • Lucey, J. A. (2002). Formation and physical properties of milk protein gels. Journal of Dairy Science, 85, 281–294.

    Article  CAS  Google Scholar 

  • Lucey, J. A. (2004). Cultured dairy products: An overview of their gelation and texture properties. International Journal of Dairy Technology, 57, 77–84.

    Article  CAS  Google Scholar 

  • Lucey, J. A., Munro, P. A., & Singh, H. (1998). Whey separation in acid milk gels made with glucono-delta-lactone: Effects of heat treatment and gelation temperature. Journal of Texture Studies, 29, 413–426.

    Article  Google Scholar 

  • Mahaut, M., & Korolczuk, J. (1992). Effect of whey protein addition and heat treatment of milk on the viscosity of UF fresh cheese. Milchwissenschaft, 47, 157–159.

    CAS  Google Scholar 

  • Modler, H. W., & Kalab, M. (1983). Microestructure of yogurt stabilized with milk proteins. Journal of Dairy Science, 63, 430–437.

    Article  Google Scholar 

  • NP 1923 (1987). Iogurte Determinação do teor de matéria gorda Técnica de Gerber. Processo corrente. Comissão Técnica—32, 1ª edição. Portugal.

  • NP 2105 (1983). Queijos. Determinação do teor de matéria gorda. Técnica de Van Gulik. Processo corrente. Comissão Técnica—32, 1ª edição, Portugal.

  • NP 3544 (1987). Queijos e queijos fundidos. Determinação do resíduo seco e do resíduo seco isento de matéria gorda. Comissão Técnica—32, 1ª edição, Portugal.

  • NP 4146 (1991). Leite e produtos lácteos. Métodos de colheita de amostras. Comissão Técnica—32, 1ª edição, Portugal.

  • NP 469 (2002). Leites. Determinação da matéria gorda (técnica de Gerber). Processo corrente. Comissão Técnica—32, 3ª edição, Portugal.

  • NP 580 (1970). Leite. Determinação do resíduo seco e do resíduo seco isento de matéria gorda. Processo corrente. Comissão Técnica—32, 1ª edição, Portugal.

  • NP 701 (1982). Iogurtes. Determinação da acidez. Comissão Técnica—32, 2ª edição, Portugal.

  • NP 703 (1982). Iogurtes. Determinação do resíduo seco e resíduo seco isento de matéria gorda. Comissão Técnica—32, 2ª edição, Portugal.

  • Pérez-Munuera, M. E., & Lluch, M. A. (1999). Study of some typical Spanish cheeses by scanning electron microscopy. Main microstructural modifications caused by freezing. Food Science and Technology International, 5(6), 515–521.

    Article  Google Scholar 

  • Phadungath, C. (2010). Basic measurements for food texture. http://science.srru.ac.th/org/food_sci/result_civil_file/BasicMeasurementforFoodTexture.pdf. Accessed 16 August 2011.

  • Punidadas, P., Feirtag, J., & Tung, M. A. (1999). Incorporating whey proteins into mozzarella cheese. International Journal of Dairy Technology, 52, 51–55.

    Article  CAS  Google Scholar 

  • Salaun, F., Mietton, B., & Gaucheron, F. (2005). Buffering capacity of dairy products. International Dairy Journal, 15, 95–109.

    Article  Google Scholar 

  • Shah, N. P. (2003). Yogurt: The product and its manufacture. In B. Caballero, L. C. Trugo, & P. M. Finglas (Eds.), Encyclopedia of food science and nutrition (2nd ed., Vol. 10). London: Academic Press, London.

    Google Scholar 

  • Smithers, G. W., Ballard, F. J., Copeland, A. D., de Silva, K. J., Dionysius, D. A., Francis, G. L., et al. (1996). New opportunities from the isolation and utilization of whey proteins. Journal of Dairy Science, 79, 1454–1459.

    Article  CAS  Google Scholar 

  • Sodini, I., Montella, J., & Tong, P. S. (2005). Physical properties of yogurt fortified with various commercial whey protein concentrates. Journal of the Science of Food and Agriculture, 85, 853–859.

    Article  CAS  Google Scholar 

  • Sodini, I., Mattas, J., & Tong, P. S. (2006). Influence of pH and heat treatment of whey on the functional properties of whey protein concentrates in yoghurt. International Dairy Journal, 16, 1464–1469.

    Article  CAS  Google Scholar 

  • Tamine, A. Y., & Robinson, R. K. (1999). Yoghurt: Science and technology (2nd ed.). Boca Raton, FL: CRC Press.

    Google Scholar 

  • Walstra, P., & van Vliet, T. (1991). On the fractal nature of particle gels. In E. Dickinson (Ed.), Food polymers, gells and colloids. London: Royal Society of Chemistry.

    Google Scholar 

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Acknowledgments

The authors would like to thank Queijaria Serqueijos, SA (Portugal) for supplying bovine whey used in trials. Thanks are also due to Fundação para a Ciência e Tecnologia (FCT), Ministério da Ciência e do Ensino Superior, Operational Program of Science and Innovation 2010, for the Ph.D. grant (SFRH/BD/36645/2007).

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Correspondence to Marta H. F. Henriques.

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Henriques, M.H.F., Gomes, D.M.G.S., Pereira, C.J.D. et al. Effects of Liquid Whey Protein Concentrate on Functional and Sensorial Properties of Set Yogurts and Fresh Cheese. Food Bioprocess Technol 6, 952–963 (2013). https://doi.org/10.1007/s11947-012-0778-9

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