Abstract
Aim of this work is to study the cryoprotective role of extracellular trehalose in the production of bakery products from frozen dough. Therefore, different levels of trehalose (up to 200 ppm) were incorporated in dough/bread samples made from white and whole-wheat flour, and their quality (loaf volume, weight loss during baking, crust and crumb color) and texture characteristics (dough, crust and crumb firmness) were examined during frozen storage. To investigate the role of trehalose on dough behavior, the sugar content (glucose, fructose, and sucrose) of dough samples composed with or without trehalose was monitored, and dough microstructure was also analyzed with scanning electron microscopy. The cryoprotective effect of trehalose was confirmed, and it was found proportional to its level for both flour types. Trehalose can improve dough behavior under freezing conditions in terms of bread volume and texture characteristics.
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References
Anon, M. C., LeBail, A., & Leon, A. E. (2004). Effect of freezing on dough ingredients. In Hui, Cornillon, Legarreta, Lim, Murrell, Nip (Eds.), Handbook of frozen foods. New York, USA: Marcel Dekker.
Bar, A. (2000). Trehalose—produced by a novel enzymatic process. Dossier prepared and submitted on behalf of Hayashibara Co., Ltd., for evaluation pursuant to the EU Novel Foods Regulation (258/97) by the UK Advisory Committee on Novel Foods and Processes, Japan.
Calvel, R., Wirtz, R. L., & MacGuire, J. J. (2001). The taste of bread (p. 32). Maryland, USA: Aspen.
Carvalheiro, F., Roseiro, J. C., & Girio, F. M. (1999). Interactive effects of sodium chloride and heat shock on trehalose accumulation and glycerol production by Saccharomyces cerevisiae. Food Microbiology, 16, 543–550.
Casey, G. P., & Foy, J. J. (1995). Yeast performance in frozen doughs and strategies for improvement. In K. Lorenz & Brummer (Eds.), Frozen and refrigerated doughs and batters. Minnesota, USA: American Association of Cereal Chemists.
Christensen, R. (1996). Multiple regression: Introduction. In Christensen (Ed.), Analysis of variance, design and regression—applied statistical methods (pp. 391–408). London, UK: Chapman & Hall.
Conrad, P. B., & De Pablo, J. J. (1999). Computer simulation of the cryoprotectant disaccharide α,α-trehalose in aqueous solution. Journal of Physical Chemistry, 103, 4049–4055.
Diniz-Mendes, L., Bernardes, E., De Araujo, P. S., Panek, A. D., & Paschoalin, V. M. F. (1999). Preservation of frozen yeast cells by trehalose. Biotechnology and Bioengineering, 65(5), 572–578.
Egan, H., Kirk, R. S., & Sawyer, R. (1981). Pearson’s chemical analysis of foods (pp. 233–236). Essex, UK: Longman.
Elbein, A. D., Pan, Y. T., Pastuszak, I., & Carroll, D. (2003). New insights on trehalose: A multifunctional molecule. Glycobiology, 13(4), 17–27.
Fik, M., & Surowka, K. (2002). Effect of prebaking and frozen storage on the sensory quality and instrumental texture of bread. Journal of the Science of Food and Agriculture, 82, 1268–1275.
Food Standards Australia New Zealand (FSANZ) (2003). Trehalose as a novel food. Final assessment report application (A453), Canberra, Australia.
Fuchigami, M., Ogawa, N., & Teramoto, A. (2002). Trehalose and hydrostatic pressure effects on the structure and sensory properties of frozen tofu soybean curd. Innovative Food Science and Emerging Technology, 3, 139–147.
Gancedo, C., & Flores, C.-L. (2004). The importance of a functional trehalose biosynthetic pathway for the life of yeasts and fungi. FEMS Yeast Research, 4, 351–359.
Giannou, V., Tzia, C., & LeBail, A. (2005). Quality and safety of frozen bakery products. In Sun (Ed.), Handbook of frozen food processing and packaging. New York, USA: Marcel Dekker.
Haines, A. H. (2003). Synthesis of L-trehalose and observations on isomer and by-product formation. Carbohydrate Research, 338, 813–818.
Hall, E., Ibanoglu, S., & Ainsworth, P. (2004). Effect of fermented/germinated cowpea flour addition on the rheological and baking properties of wheat flour. Journal of Food Engineering, 63, 177–184.
Holmes, J. T., & Hoseney, R. C. (1987).Frozen doughs: Freezing ant thawing rates and the potential of using a combination of yeast and chemical leavening. Cereal Chemistry, 64(4), 348–351.
Kalichevsky-Dong, M. T. (2000). The glass transition and microbial stability. In Kilcast & Subramaniam (Eds.), The stability and shelf-life of food. Cambridge, UK: Woodhead and CRC Press LLC.
Karel, M., & Lund, D. B. (2003). Freezing. In Karel & Lund (Eds.), Physical principles of food preservation. New York, USA: Marcel Dekker.
Kim, H.-J., Morita, N., Lee, S.-H., & Moon, K.-D. (2003). Scanning electron microscopic observations of dough and bread supplemented with Gastrodia elata Blume powder. Food Research International, 36, 387–397.
Laaksonen, T. J., & Roos, Y. H. (2000). Thermal, dynamic-mechanical, and dielectric analysis of phase and state transitions of frozen wheat doughs. Journal of Cereal Science, 32, 281–292.
MacDougall, D. B. (2002). Colour measurement of food, principles and practice. In MacDougall (Ed.), Colour in food, improving quality. Cambridge, UK: Woodhead.
Masaru, O., Naoki, U., & Norifumi, O. (1995). Method for producing bread. European Patent Application, EP0688501.
Meilgaard, M. C., Civille, G. V., & Carr, B. T. Descriptive analysis techniques (Ch. 10). In: Sensory evaluation techniques (3rd ed.). New York, USA: CRC Press; 1999.
Meric, L., Lambert-Guilois, S., Neyreneuf, O., & Richard-Molard, D. (1995). Cryoresistance of baker’s yeast Saccharomyces cerevisiae in frozen dough: Contribution of cellular trehalose. Cereal Chemistry, 72(6), 609–615.
Neyreneuf, O., & Delpuech, B. (1993). Freezing experiments on yeasted dough slabs, Effect of cryogenic temperatures on the baking performance. Cereal Chemistry, 70(1), 109–111.
Pyler, E. J. (1988). Baking science & technology, vol II (3rd ed., p. 590–592). Missouri, USA: Sosland.
Ribotta, P. D., Leon, A. E., & Anon, M. C. (2001). Effect of freezing and frozen storage of doughs on bread quality. Journal of Agricultural and Food Chemistry, 49(2), 913–918.
Ribotta, P. D., Perez, G. T., Leon, A. E., & Anon, M. C. (2004). Effect of emulsifier and guar gum on micro structural, rheological and baking performance of frozen bread dough. Food Hydrocolloids, 18, 305–313.
Roos, Y. (1995). Characterization of food polymers using state diagrams. Journal of Food Engineering, 24, 339–360.
Rosenthal, A. J. (1999). Relation between instrumental and sensory measures of food texture. In: Rosenthal (Ed.), Food texture—measurement and perception (pp. 1–13). Maryland, USA: Aspen.
Roser, B. (1991). Trehalose, a new approach to premium dried foods. Trends in Food Science and Technology, 2, 166–169.
Rouille, J., LeBail, A., & Courcoux, P. (2000). Influence of formulation and mixing conditions on breadmaking qualities of french frozen dough. Journal of food engineering, 43, 197–203.
Schiraldi, C., Di Lernia, I., & De Rosa, M. (2002). Trehalose production: Exploiting novel approaches. Trends in Biotechnology, 20(10), 420–425.
Sharma, S. (1996). Principal component analysis. In Sharma (Ed.), Applied multivariate techniques (p. 58–81). New York, USA: Wiley.
Shimizu, Y., Maeda, T., Hidaki, Y., Tani, H., & Morita, N. (2003). Identification and effect of ethyl galactoside on the properties and baking quality of dough. Food Research International, 36, 373–379.
Singer, M. A., & Lindquist, S. (1998). Thermotolerance in Saccharomyces cerevisiae: The Yin and Yang of trehalose. Trends in Biotechnology, 16, 460–468.
StatSoft Inc. (2006). Electronic statistics textbook. Oklahoma, USA: Tulsa.
Tanghe, A., Teunissen, A., Van Dijck, P., & Thevelein, J. M. (2000). Identification of genes responsible for improved cryoresistance in fermenting yeast cells. International Journal of Food Microbiology, 55, 259–262.
Van Dijck, P., Colavizza, D., Smet, P., & Thevelein, J. M. (1995). Differential importance of trehalose in stress resistance in fermenting and nonfermenting saccharomyces cerevisiae cells. Applied and Environmental Microbiology, 61(1), 109–115.
Verstrepen, K. J., Iserentant, D., Malcorps, P., Derdelinckx, G., Van Dijck, P., Winderickx, J., et al. (2004). Glucose and sucrose: Hazardous fast-food for industrial yeast? Trends in Biotechnology, 22(10), 531–537.
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The present work has been supported by grants from Greek GSRT (General Secretariat of Research and Technology).
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Giannou, V., Tzia, C. Cryoprotective Role of Exogenous Trehalose in Frozen Dough Products. Food Bioprocess Technol 1, 276–284 (2008). https://doi.org/10.1007/s11947-007-0008-z
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DOI: https://doi.org/10.1007/s11947-007-0008-z