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The central role of conjunctival mast cells in the pathogenesis of ocular allergy

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Abstract

Ocular allergic diseases are characterized by specific activation of conjunctival mast cells with subsequent release of preformed and newly formed mediators. Mast-cell numbers on the ocular surface are increased in all forms of allergic conjunctivitis. Mast-cell activation plays a central role in the development of the ocular allergic reaction, which can be divided into an early and a late inflammatory phase. Mastcell mediators have been measured in tears of patients suffering from various forms of allergic conjunctivitis, and in sensitized patients after specific ocular allergen challenge. Histamine and tryptase are the most studied mediators in tears of allergic patients. Several cytokines, such as IL-4 and TNFα are also produced and released by conjunctival mast cells, and probably play a role in the immunoregulation on the ocular surface. In vitro studies of the characteristics and biologic functions of conjunctival mast cells highlight their central role in the pathogenesis of ocular allergy, and have led to new opportunities to evaluate anti-allergic compounds. This review discusses the role of conjunctival mast cells in the development of ocular allergic diseases.

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References and Recommended Reading

  1. Craviotto C, Grossi G: On the critical analysis of the moment of the mastocytes in special clinical and experimental ophthalmopathic conditions. II. 11 a. Study of the relation between the degranulation of the mastocyte and the eosinophilic granulocyte in conjunctival biopsies [Italian]. Riv Anat Patol Oncol 1969, 35:167–183.

    PubMed  CAS  Google Scholar 

  2. Takakusaki I: Fine structure of the human palperbral conjunctiva with special reference to the pathological changes in vernal conjunctivitis. Arch Histol Jpn 1969, 30:247–282.

    PubMed  CAS  Google Scholar 

  3. Radnot M: Mast cell ultrastructure in the ocular globe adnexa [French]. Arch Ophtalmol Rev Gen Ophtalmol 1972, 32:303–309.

    PubMed  CAS  Google Scholar 

  4. Allansmith MR, Greiner JV, Baird RS: Number of inflammatory cells in the normal conjunctiva. Am J Ophthalmol 1978, 86:250–259.

    PubMed  CAS  Google Scholar 

  5. Allansmith MR, Korb DR, Greiner JV: Giant papillary conjunctivitis induced by hard or soft contact lens wear: quantitative histology. Ophthalmology 1978, 85:766–778.

    PubMed  CAS  Google Scholar 

  6. Henriquez AS, Kenyon KR, Allansmith MR: Mast cell ultrastructure. Comparison in contact lens-associated giant papillary conjunctivitis and vernal conjunctivitis. Arch Ophthalmol 1981, 99:1266–1272.

    PubMed  CAS  Google Scholar 

  7. Allansmith MR, Baird RS: Percentage of degranulated mast cells in vernal conjunctivitis and giant papillary conjunctivitis associated with contact-lens wear. Am J Ophthalmol 1981, 91:71–75.

    PubMed  CAS  Google Scholar 

  8. Irani AM, Butrus SI, Tabbara KF, Schwartz LB: Human conjunctival mast cells: distribution of MCT and MCTC in vernal conjunctivitis and giant papillary conjunctivitis. J Allergy Clin Immunol 1990, 86:34–40.

    Article  PubMed  CAS  Google Scholar 

  9. Morgan SJ, Williams JH, Walls AF, et al.: Mast cell numbers and staining characteristics in the normal and allergic human conjunctiva. J Allergy Clin Immunol 1991, 87:111–116.

    Article  PubMed  CAS  Google Scholar 

  10. Miller S, Cook E, Graziano F, et al.: Human conjunctival mast cell responses in vitro to various secretagogues. Ocul Immunol Inflamm 1996, 4:39–49.

    Article  Google Scholar 

  11. Morgan SJ, Williams JH, Walls AF, Holgate ST: Mast cell hyperplasia in atopic keratoconjunctivitis. An immunohistochemical study. Eye 1991, 5:729–735.

    PubMed  Google Scholar 

  12. Anderson DF, MacLeod JD, Baddeley SM, et al.: Seasonal allergic conjunctivitis is accompanied by increased mast cell numbers in the absence of leucocyte infiltration. Clin Exp Allergy 1997, 27:1060–1066.

    Article  PubMed  CAS  Google Scholar 

  13. Schwartz LB, Huff TF: Biology of mast cells. In Allergy: Principles & Practice, edn 5. Edited by Middleton E, Reed CE, Ellis EF, et al. St Louis: Mosby/Elsevier Science; 1998:261–276.

    Google Scholar 

  14. Abelson MB, Baird RS, Allansmith MR: Tear histamine levels in vernal conjunctivitis and other ocular inflammations. Ophthalmology 1980, 87:812.

    PubMed  CAS  Google Scholar 

  15. Abelson MB, Leonardi A, Fregona et al.: Histaminase activity in patients with vernal keratoconjunctivitis. Ophthalmology 1995, 102:1958.

    PubMed  CAS  Google Scholar 

  16. Abelson MB, Chambers W, Smith LM: Conjunctival allergen challenge. Arch Ophthalmol 1990, 108:84.

    PubMed  CAS  Google Scholar 

  17. Kari O, Salo OP, Hamepuro L, Suvilehto K: Tear histamine during allergic conjunctivitis challenge. Graefes Arch Clin Exp Ophthalmol 1985, 223:60–62.

    Article  PubMed  CAS  Google Scholar 

  18. Leonardi A, Smith LM, Fregona IA, Secchi AG: Tear histamine and histaminase during the early (EPR) and late (LPR) phases of the allergic reaction and the effect of lodoxamide. Eur J Ophthalmol 1996, 6:106.

    PubMed  CAS  Google Scholar 

  19. Leonardi A: Role of histamine in allergic conjunctivitis. Acta Ophthalmol Scand Suppl 2000, 78:18–21.

    Article  Google Scholar 

  20. Abelson MB, Allansmith MR: Histamine and the eye. In Immunology and Immunopathology of the Eye. Edited by Silverstein A, O’Connor G. New York: Masson Publishing USA Inc.; 1979.

    Google Scholar 

  21. Bonini S, Bonini S, Schiavone M, et al.: Conjunctival hyperresponsiveness to ocular histamine challenge in patients with vernal conjunctivitis. J Allergy Clin Immunol 1992, 89:103–107.

    Article  PubMed  CAS  Google Scholar 

  22. Ciprandi G, Buscaglia S, Pesce GP, et al.: Ocular challenge and hyperresponsiveness to histamine in patients with allergic conjunctivitis. J Allergy Clin Immunol 1993, 91:1227–1230.

    Article  PubMed  CAS  Google Scholar 

  23. Leonardi A, Bloch KJ, Briggs R, Allansmith MR: Histology of ocular late-phase reaction in guinea pigs passively sensitized with IgG1 antibodies. Ophthalmic Res 1990, 22:209–219.

    Article  PubMed  CAS  Google Scholar 

  24. Helleboid L, Khatami M, Wei ZG, Rockey JH: Histamine and prostacyclin. Primary and secondary release in allergic conjunctivitis. Invest Ophthalmol Vis Sci 1991, 32:2281–2289.

    PubMed  CAS  Google Scholar 

  25. Leonardi A, Secchi AG, Briggs R, Allansmith MR: Conjunctival mast cells and the allergic late phase reaction. Ophthalmic Res 1992, 24:234–242.

    PubMed  CAS  Google Scholar 

  26. Butrus SI, Ochsner KI, Abelson MB, Schwartz LB: The level of tryptase in human tears. An indicator of activation of conjunctival mast cells. Ophthalmology 1990, 97:1678–1683.

    PubMed  CAS  Google Scholar 

  27. Bacon AS, Ahluwalia P, Irani AM, et al.: Tear and conjunctival changes during the allergen-induced early- and late-phase responses. J Allergy Clin Immunol 2000, 106:948–954. Results of this study confirm that conjunctival early- and late-phase reactions are mast-cell dependent and reproducible, and associated with characteristic tear and tissue changes.

    Article  PubMed  CAS  Google Scholar 

  28. Fukagawa K, Saito H, Azuma N, et al.: Histamine and tryptase levels in allergic conjunctivitis and vernal keratoconjunctivitis. Cornea 1994, 13:345–348.

    Article  PubMed  CAS  Google Scholar 

  29. Tabbara KF: Tear tryptase in vernal keratoconjunctivitis. Arch Ophthalmol 2001, 119:338–342.

    PubMed  CAS  Google Scholar 

  30. Leonardi A, Busato F, Fregona I, et al.: Anti-inflammatory and antiallergic effects of ketorolac tromethamine in the conjunctival provocation model. Br J Ophthalmol 2000, 84:1228–1232.

    Article  PubMed  CAS  Google Scholar 

  31. Butrus S, Wun PJ: Mast cell tryptase: its role in ocular allergic inflammation. In Allergic Diseases of the Eye. Edited by Abelson MB. Philadelphia: W.B. Saunders; 2000:103–106.

    Google Scholar 

  32. Abelson MB, Grant KK, Chapin MJ: Leukotrienes. In Allergic Diseases of the Eye. Edited by Abelson MB. Philadelphia: W.B. Saunders; 2000:93–97.

    Google Scholar 

  33. Abelson MB, Grant KK, Chapin MJ: Prostaglandins. In Allergic Diseases of the Eye. Edited by Abelson MB. Philadelphia: W.B. Saunders; 2000:99–101.

    Google Scholar 

  34. Cook EB, Stahl JL, Miller ST, et al.: Isolation of human conjunctival mast cells and epithelial cells: tumor necrosis factor-alpha from mast cells affects intercellular adhesion molecule 1 expression on epithelial cells. Invest Ophthalmol Vis Sci 1998, 39:336–343. First paper showing interactions between cultured conjunctival mast cells and epithelial conjunctival cells. A new in vitro model to study ocular allergy.

    PubMed  CAS  Google Scholar 

  35. Lambiase A, Bonini S, Micera A, et al.: Increased plasma levels of substance P in vernal keratoconjunctivitis. Invest Ophthalmol Vis Sci 1997, 38:2161–2164.

    PubMed  CAS  Google Scholar 

  36. Lambiase A, Bonini S, Bonini S, et al.: Increased plasma levels of nerve growth factor in vernal keratoconjunctivitis and relationship to conjunctival mast cells. Invest Ophthalmol Vis Sci 1995, 36:2127–2132.

    PubMed  CAS  Google Scholar 

  37. Baddeley SM, Bacon AS, McGill JI, et al.: Mast cell distribution and neutral protease expression in acute and chronic allergic conjunctivitis. Clin Exp Allergy 1995, 25:41–50.

    Article  PubMed  CAS  Google Scholar 

  38. Macleod JD, Anderson DF, Baddeley SM, et al.: Immunolocalization of cytokines to mast cells in normal and allergic conjunctiva. Clin Exp Allergy 1997, 27:1328–1334.

    Article  PubMed  CAS  Google Scholar 

  39. Zhang S, Anderson DF, Bradding P, et al.: Human mast cells express stem cell factor. J Pathol 1998, 186:59–66.

    Article  PubMed  CAS  Google Scholar 

  40. Anderson DF, Zhang S, Bradding P, et al.: The relative contribution of mast cell subsets to conjunctival T(H)2-like cytokines. Invest Ophthalmol Vis Sci 2001, 42:995–1001. The different pattern of cytokine expression in conjunctival mast cells demonstrated in this article suggest further and different imuunoregulative properties of conjunctival mast cell subtypes.

    PubMed  CAS  Google Scholar 

  41. Gauchat JF, Henchoz S, Mazzei G, et al.: Induction of human IgE synthesis in B cells by mast cells and basophils. Nature 1993, 365:340–343.

    Article  PubMed  CAS  Google Scholar 

  42. Uchio E, Ono SY, Ikezawa Z, Ohno S: Tear levels of interferong, interleukin (IL) -2, IL-4, and IL-5 in patients with vernal keratoconjunctivitis, atopic keratoconjunctivitis and allergic conjunctivitis. Clin Exp Allergy 2000, 30:103–109.

    Article  PubMed  CAS  Google Scholar 

  43. Weimer LK, Gamache DA, Yanni JM: Histamine-stimulated cytokine secretion from human conjunctival epithelial cells: inhibition by the histamine H1 antagonist emedastine. Int Arch Allergy Immunol 1998, 115:288–293.

    Article  PubMed  CAS  Google Scholar 

  44. Leonardi A, Brun P, Tavolato M, et al.: Growth factors and collagen distribution in vernal keratoconjunctivitis. Invest Ophthalmol Vis Sci 2000, 41:4175–4181. This paper, showing that conjunctival mast cells express growth factors, suggests the potential effects of mast cells on tissue remodeling in vernal keratoconjunctivitis.

    PubMed  CAS  Google Scholar 

  45. Leonardi A, Radice M, Fregona IA, et al.: Histamine effects on conjunctival fibroblasts from patients with vernal conjunctivitis. Exp Eye Res 1999, 68:739–746.

    Article  PubMed  CAS  Google Scholar 

  46. Stahl JL, Cook EB, Graziano FM, Barney NP: Human conjunctival mast cells: expression of Fc epsilonRI, c-kit, ICAM-1, and IgE. Arch Ophthalmol 1999, 117:493–497. Characterization of the expression and regulation of mast-cell surface receptors may be important to a further development of new potential treatments.

    PubMed  CAS  Google Scholar 

  47. Yanni JM, Miller ST, Gamache DA, et al.: Comparative effects of topical ocular anti-allergy drugs on human conjunctival mast cells. Ann Allergy Asthma Immunol 1997, 79:541–545.

    Article  PubMed  CAS  Google Scholar 

  48. Abelson MB, Spitalny L: Combined analysis of two studies using the conjunctival allergen challenge model to evaluate olopatadine hydrochloride, a new ophthalmic antiallergic agent with dual activity. Am J Ophthalmol 1998, 125:797–804.

    Article  PubMed  CAS  Google Scholar 

  49. Whitcup SM, Chan CC, Luyo DA, et al.: Topical cyclosporine inhibits mast cell-mediated conjunctivitis. Invest Ophthalmol Vis Sci 1996, 37:2686–2693.

    PubMed  CAS  Google Scholar 

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  1. Department of Ophthalmology and Department of Laboratory Medicine, University of Padova, via Foscari 8, 35127, Padova, Italy

    Andrea Leonardi MD

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Leonardi, A. The central role of conjunctival mast cells in the pathogenesis of ocular allergy. Curr Allergy Asthma Rep 2, 325–331 (2002). https://doi.org/10.1007/s11882-002-0061-7

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  • DOI: https://doi.org/10.1007/s11882-002-0061-7

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